Comparison of Antibiotic Sensitivity Pattern of Escherichia coli which Produce Extended Spectrum Beta-Lactamase Strains Isolated From Various Clinical Specimens In Intensive Care Unit

Abstract

Objective: A retrospective analysis of the widely used antibiotics all susceptibility testing results from Escherichia coli (E. coli) cultured from clinical specimens' private hospital (from January 2017 to November 2018) was performed. Methods: The VITEK 2 Compact automated microbiology system (bioMérieux) is designed for automated rapid antimicrobial susceptibility testing and identification of clinically relevant bacteria. Minimum inhibitory concentration (MIC) results previously obtained in recent clinical isolates with well-defined in isolates with well-characterized resistance mechanisms with the microdilution method were re-interpreted for the susceptible, intermediate and resistant categories using the 2018 EUCAST breakpoints. Clinical samples are most commonly isolated from blood, sputum and urine samples. Results: The results of resistance pattern of E. coli isolates in our locality to antimicrobial agents showed that the 64 E. coli strains tested against fifteen antimicrobial agents. E. coli isolates were highly resistant to piperacillin, ceftazidime and aztreonam 98%, 61% and 61% respectively. The most sensitive antibiotics were colistin, tigecycline, imipenem and meropenem. In the present study, 73% (47) of the isolates were resistant to at least three to fourteen antibiotics. All the isolates showed resistance to at least one antibiotic. Thirtynine per cent of E. coli isolates were extended spectrum beta-lactamase (ESBL) producers. Conclusion: Considering the antibiogram, imipenem and meropenem should be preferred drugs for E. coli infection isolated from clinical samples.