Comparison of antibacterial activity of Ag nanoparticles synthesized from leaf extract ofParthenium hystrophorusL in aqueous media and Gentamicin sulphate:in-vitro

Abstract

Monodisperse silver (Ag) nanoparticles were synthesized by using Parthenium hystrophorus L leaf extract in aqueous media. The synthesized nanoparticles were characterized by using UV-vis spectrophotometer, X-ray diffracto-meter (XRD), transmission electron microscope (TEM), and dynamics light scattering (DLS). Size-dependent antibacterial activities of Ag nanoparticles were tested against Gram negative Pseudomonas aeruginosa and Gram positive Staphylococcus aureus. Ag nanoparticles having 20 ± 2 nm size in diameter show maximum zone of inhibition (23 ± 2.2 mm) in comparison to 40 nm and 70 nm diameter nanoparticles for Pseudomonas aeruginosa. The zone of inhibition against Staphylococcus aureus were 19 ± 1.8 mm, 15 ± 1.5 mm and 11 ± 1 mm for 20 nm, 40 nm, and 70 nm, respectively. In addition, affect of concentration of 20 nm size Ag nanoparticles on Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus species were also reported and results were compared with 10 µg/ml dose of Gentamicin sulphate. The Parthenium hystrophorus L leaf extract capped 20 ± 2 nm Ag nanoparticles (7.5 µg/ml) shows statistically significant antibacterial activity than Gentamicin sulphate (10 µg/ml) against Staphylococcus aureus.

Cocoa pod husk extract-mediated biosynthesis of silver nanoparticles: its antimicrobial, antioxidant and larvicidal activities

“…For instance, metabolites of some arthropods have been used for green synthesis of nanoparticles [2][3][4][5]. In several studies, metabolites and enzymes of microbial origin (fungi and bacteria), even whole microbes have been used in the synthesis of nanoparticles [6][7][8][9][10][11], while many others have reported the synthesis of nanoparticles by employing extracts from various parts of the plants such as seeds, fruits, flowers, leaves, stem and roots [12][13][14][15][16][17][18][19]. Kaviya et al [20], Njagi et al [21] and Roopan et al [22] synthesized AgNPs using Sorghum spp bran extract, Citrus cinensis peel extract and Cocos nucifera coir, respectively, suggesting the usefulness of agro-wastes in green nanotechnology.…”

Vermicomposting transforms allelopathic parthenium into a benign organic fertilizer

“…Equally futile have been the attempts to find ways for gainfully utilizing parthenium. Even though a large number of options have been explored d for making parthenium a source of biopesticides (Datta and Saxena, 2001;Sreekanth, 2013;, green manure (Kishor et al, 2010;Kumar et al, 2012), energy (Patel, 2011;Singh and Garg, 2014;Tavva et al, 2016), essential oils (Miranda et al, 2016); drugs (Kumar et al, 2014a,b;Anwar et al, 2015;Singh and Beck, 2006), and nanoparticles (Abbasi et al, 2015a,b) d none of the methods has had economic viability. As a result, billions of tonnes of parthenium biomass that is generated annually across the world, remains unutilized.…”

Transformation of the pernicious and toxic weed parthenium into an organic fertilizer by vermicomposting

“…Attempts to find ways of using parthenium as a source of biopecticide [14,15], green manure [16,17], biogas production [9,18], drugs [19,20], and nanoparticles [21] have all failed to check its proliferation. As a result, millions of tonnes of parthenium biomass remain unused, contributing to global warming as the debris and the dead plants of parthenium degrade aerobically/anaerobically in the open, releasing CO 2 and CH 4 [22][23][24].…”