Comparison of Anthracnose Resistance with the Presence of Two SCAR Markers Associated with the Rca2 Gene in Strawberry

Miller-Butler, Melinda A.; Smith, Barbara J.; Kreiser, Brian R.; Blythe, Eugene K.

doi:10.21273/hortsci13805-18

Cited by 10 publications

(5 citation statements)

References 24 publications

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“…To date, there are no 2 cultivars that exhibiting complete resistance to petiole and/or crown rot caused by Colletotrichum. (Anciro et al, 2018;Miller-Butler et al, 2019).…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

Colletotrichum siamense infection caused transcripts involved plant hormone signal transduction and phenylpropanoid biosynthesis varied in strawberry

Zheng

Shu

Luo

2022

Not Bot Horti Agrobo

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Colletotrichum siamense infected the petiole and crown of strawberry (Fragaria × ananassa), and this anthracnose threatens strawberry production heavily in Hubei province. Characteristic the variation of transcript caused by C. siamense is important for anthracnose control of strawberry. In this study, RNA-seq analysis was used to monitor the strawberry transcripts in response of C. siamense at 0- and 6-days post-inoculation based on lesion measurement and hypha observation. The number of clean reads achieved for each sample was ranged from 5.68 to 8.12 Gb. The 0DPM vs 0DPI group had seven significantly differentially expressed transcripts, whereas the 6DPM vs 6DPI group exhibited 12,097 significantly differentially expressed transcripts. The differentially expressed transcripts of 6DPM vs 6DPI group were enriched in ‘metabolic process’ and ‘biological process’ in the biological process category and ‘oxidoreductase activity’ in the molecular function category by GO analysis. While ‘Starch and sucrose metabolism’, ‘Plant hormone signal transduction’ and ‘Phenylpropanoid biosynthesis’ pathways were significantly enriched by KEGG analysis. The expression of MYC2 in JA, the ETR in Eth and, the SnRK2 and ABF in ABA signal transduction pathways were promoted, whereas expression of the PR1 in SA, JZA1 in JA, and ERF in Eth signal transduction pathways were inhibited. In addition, the transcripts that encode enzymes involved in the ‘Phenylpropanoid biosynthesis’ pathway, such as PAL, CYP73A, CSE, 4CL, HTC, GSE and COMT were all inhibited showed by RNA-seq and qRT-PCR. This study provided basic information of strawberry responding to C. siamense infection.

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“…To date, there are no 2 cultivars that exhibiting complete resistance to petiole and/or crown rot caused by Colletotrichum. (Anciro et al, 2018;Miller-Butler et al, 2019).…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

Colletotrichum siamense infection caused transcripts involved plant hormone signal transduction and phenylpropanoid biosynthesis varied in strawberry

Zheng

Shu

Luo

2022

Not Bot Horti Agrobo

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“…Проведённый ранее (Lyzhin, Luk'yanchuk, 2022a; b) анализ исходных родительских форм земляники на наличие диагностического маркера STS-Rca2_240 показал, что сорт 'Roxana' характеризуется гетерозиготным сочетанием аллелей гена Rca2, у сортов 'Aprica' и 'Monterey' аллель резистентности Rca2 находится в гомозиготном или гетерозиготном состоянии, сорта 'Faith', ' Asia', 'Quicky', 'Vicoda' имеют гомозиготный генотип по аллелю rca2. Присутствие диагностического маркера STS-Rca2_240 у сорта 'Roxana' подтверждается также работами других авторов (Miller-Butler et al, 2019;Khrabrov et al, 2021). Для сортов 'Алиса', 'Florence', 'San Andreas' и 'Olympia' данные об аллельном состоянии гена Rca2 отсутствуют.…”

Section: Discussionunclassified

Identification of Rca2 and Rpf1 resistance alleles in strawberry breeding forms of intervarietal hybrid origin

Lyzhin,

Luk’yanchuk

2024

Biotehnologiâ i selekciâ rastenij

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Background. The creation of varieties with genetic resistance to fungal pathogens is one of the most important directions in strawberry breeding. A promising direction of accelerated selection of valuable specimens is molecular screening for the presence in their genotype of diagnostic DNA markers, linked to target genes of agrobiological traits. Objectives of the work were determination of the allelic state of the Rca2 (resistance to anthracnose) and Rpf1 (resistance to red stele root rot) genes in selected strawberry forms to identify genotypes resistant to Colletotrichum acutatum Simmonds and Phytophthora fragariae var. fragariae Hickman.Material and methods. The object of research was breeding forms of strawberries of intervarietal hybrid origin. As a result of 22 crossing combinations, 61 selection forms were obtained, in which the allelic composition of resistance genes was studied. The Rca2 anthracnose resistance allele was identified by means of the STS-Rca2_240 marker. The Rpf1 red stele root rot resistance allele was identified with the help of SCAR-R1A marker. Results. At least one of the two genes was identified in 20 selected strawberry forms (32.8%). The STS-Rca2_240 marker was identified in 15 strawberry seedlings (22.9%) from six hybrid combinations ‘Vicoda’ × ‘Roxana’, ‘Florence’ × ‘Faith’, ‘Asia’ × ‘Aprica’, ‘San Andreas’ × ‘Monterey’, ‘Alisa’ × ‘Quicky’, ‘Quicky’ × ‘Olympia’). The SCAR-R1A marker was identified in five strawberry seedlings (8.2%) from four hybrid combinations (‘Privlekatelnaya’ × ‘Bylinnaya’, ‘Feyerverk’ × ‘Bylinnaya’, ‘Bylinnaya’ × ‘Feyerverk’, ‘Bylinnaya’ × ‘Olimpiyskaya nadezhda’). It has been established that all strawberry selected forms with identified marker fragments for resistance genes are characterized by a heterozygous combination of alleles.Conclusions. Promising sources of resistance alleles are strawberry selected forms: 2/1-24, 2/1-34 (‘Quicky’ × ‘Olympia’), 3/9-5 (‘Florence’ × ‘Faith’), 3/12-2, 3/12- 9 (‘Alisa’ × ‘Quicky’), 4/7-10, 4/7-19, 4/7-20 (‘Asia’ × ‘Aprica’), 5/2-26, 5/2-32 (‘San Andreas’ × ‘Monterey’), 9/ 1-11, 9/1-12, 9/1-13, 9/1-32, 9/1-37 ‘Vicoda’ × ‘Roxana’) in case of Rca2 gene; 72-24, 72-71 (Privlekatelnaya × Bylinnaya), 69-29 (‘Feyerverk’ × ‘Bylinnaya’), 62-41 (‘Bylinnaya’ × ‘Feyerverk’), 61-15 (‘Bylinnaya’ × ‘Olimpiyskaya nadezhda’) in case of Rpf1 gene.

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“…Она контролируется доминантным геном Rca2 (Guérin et al, 2003;Lerceteau-Köhler et al, 2005), для детекции которого были разработаны маркеры STS-Rca2_240 и STS-Rca_417. Эффективность этих маркеров некоторыми авторами оспаривается (Miller-Butler et al, 2019), однако они достаточно активно используются как зарубежными, так и отечественными исследователями для скрининга коллекций (Lerceteau-Köhler et al, 2005;Njuguna, 2010;Sturzeanu et al, 2017;Lukyanchuk et al, 2018;Lyzhin et al, 2019;Khrabrov et al, 2019).…”

Section: Molecular Screening Of the Vir Strawberry Variety Collection...unclassified

“…Для ряда зарубежных сортов ('Albritton', 'Blakemore', 'Cambridge Favourite', 'Catskill', 'Earlibelle', 'Fairfax', 'Gorella', 'Grenadier', 'Holiday', 'Honeoye', 'Hood', 'Red Gauntlet', 'Red Chief', 'Senga Sengana', 'Sequoia', 'Stelemaster', 'Surecrop') и для отечественного сорта 'Фестивальная' молекулярный скрининг на наличие маркера Rca2_240 был ранее проведен и другими исследователями (Sturzeanu at al., 2016;Njuguna, 2010;Miller-Butler et al, 2019;Lyzhin et al, 2019;Lukyanchuk et al, 2018;Lerceteau-Köhler et al, 2005). Для подавляющего большинства сортов наши результаты совпали с данными других авторов (Lerceteau-Köhler et al, 2005;Njuguna, 2010;Miller-Butler et al, 2019).…”

Section: Fig 2 Pcr Products Obtained During Amplification Of Strawber...unclassified

Molecular screening of the VIR strawberry varieties collection for the presence of a marker for the anthracnose black rot resistance gene Rca2

Храбров

Antonova

Shapovalov

et al. 2022

Biotehnologiâ i selekciâ rastenij

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Background. Anthracnose black rot caused by the phytopathogenic fungus Colletotrichum acutatum Simmonds became an extremely harmful disease of strawberries in Southern Russia. The disease is widespread throughout the world, and relatively recently it appeared in Russia. Yield losses due to the disease reach up to 80%; besides, the pathogen causes significant plant losses in mother plantations. The most reliable protection against the pathogen is the cultivation of resistant varieties. In strawberries, resistance is controlled by different genes, including Rca2. To identify this gene, molecular markers STS_Rca2_240 and STS-Rca2_417 have been developed. The purpose of this study was to use the markers for screening the VIR collection of strawberry varieties at the VIR Maikop Experiment Station (Maikop ES VIR). Material and methods. The present work studied 135 varieties of Fragaria × ananassa (Duchesne ex Weston) Duchesne ex Rozier, 83 domestic and 52 foreign ones. The domestic varieties included 17 created at the Maikop ES. Plants were evaluated for anthracnose field resistance in the Republic of Adygea from 2018 to 2021. Molecular screening was performed using STS_Rca2_240 and STS-Rca2_417, the molecular markers closely linked to the Rca2 gene. Microsatellite primers EMFv020 were used to control the PCR. The cultivar ‘Sudarushka’, in which the presence of STS_Rca2_240 marker was described in the literature, served as a positive control. Results and discussion. The marker Rca2_240 was detected in 22 cultivars from 135 studied. Among domestic varieties, the frequency of the marker was 18.1%, while among the foreign varieties it was slightly lower and amounted to 13.0%. Among the 17 varieties created at the Maykop SE, the marker was found in three: ‘Majkopskaya rannyaya’, ‘Peryt’, and ‘Shapsugskaya’. The association of the diagnostic fragment with resistance was 73.0%. The marker efficiency was not very high due to the significant number of resistant varieties which do not generate the diagnostic fragments. The resistance in such varieties can be provided by other genes, for example, FaRCa1. The STS-Rca2_417 marker was not effective during screening. Conclusion. Twenty-two varieties with the STS_Rca2_240 marker were identified in the VIR collection, maintained at the Maykop ES VIR, 16 of which were resistant to C. acutatum. These varieties represent a valuable breeding material. The STS-Rca2_240 marker can be used as an important diagnostic trait for the certification of varieties.

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Comparison of Anthracnose Resistance with the Presence of Two SCAR Markers Associated with the Rca2 Gene in Strawberry

Cited by 10 publications

References 24 publications

Colletotrichum siamense infection caused transcripts involved plant hormone signal transduction and phenylpropanoid biosynthesis varied in strawberry

Colletotrichum siamense infection caused transcripts involved plant hormone signal transduction and phenylpropanoid biosynthesis varied in strawberry

Identification of <i>Rca2</i> and <i>Rpf1</i> resistance alleles in strawberry breeding forms of intervarietal hybrid origin

Molecular screening of the VIR strawberry varieties collection for the presence of a marker for the anthracnose black rot resistance gene <i>Rca2</i>

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