Comparison of aneuploidy rates between conventional in vitro fertilization and intracytoplasmic sperm injection in in vitro fertilization–intracytoplasmic sperm injection split insemination cycles

Kuyoro, Olutunmike; Zhao, Qianying; Behr, Barry; Lathi, Ruth B.

doi:10.1016/j.xfre.2020.07.006

Cited by 10 publications

(18 citation statements)

References 26 publications

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“…Moreover, as per blastocysts obtained, sperm factors did not exert significant effects on the euploidy rate, although using poor-quality sperm for insemination appears to reduce fertilization and blastocyst formation rates ( Mazzilli et al, 2017 ). Furthermore, univariate GEE analysis demonstrated that other patient or cycle variables evaluated in this study, such as BMI, AMH, ovarian stimulation protocols, and artificial insemination methods, were not significantly correlated with embryo ploidy; these findings were similar to previous reports ( Deng et al, 2020 ; Irani et al, 2020 ; Cascales et al, 2021 ; Pipari et al, 2021 ; Stovezky et al, 2021 ).…”

Section: Discussionsupporting

confidence: 90%

Blastocyst Morphology Based on Uniform Time-Point Assessments is Correlated With Mosaic Levels in Embryos

Chen¹,

Lee

Huang³

et al. 2021

Front. Genet.

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Avoiding aneuploid embryo transfers has been shown to improve pregnancy outcomes in patients with implantation failure and pregnancy loss. This retrospective cohort study aims to analyze the correlation of time-lapse (TL)-based variables and numeric blastocyst morphological scores (TLBMSs) with different mosaic levels. In total, 918 biopsied blastocysts with time-lapse assessments at a uniform time-point were subjected to next-generation sequencing–based preimplantation genetic testing for aneuploidy. In consideration of patient- and cycle-related confounding factors, all redefined blastocyst morphology components of low-grade blastocysts, that is, expansion levels (odds ratio [OR] = 0.388, 95% confidence interval [CI] = 0.217–0.695; OR = 0.328, 95% CI = 0.181–0.596; OR = 0.343, 95% CI = 0.179–0.657), inner cell mass grades (OR = 0.563, 95% CI = 0.333–0.962; OR = 0.35, 95% CI = 0.211–0.58; OR = 0.497, 95% CI = 0.274–0.9), and trophectoderm grades (OR = 0.29, 95% CI = 0.178–0.473; OR = 0.242, 95% CI = 0.143–0.411; OR = 0.3, 95% CI = 0.162–0.554), were less correlated with mosaic levels ≤20%, <50%, and ≤80% as compared with those of top-grade blastocysts (p < 0.05). After converting blastocyst morphology grades into scores, high TLBMSs were associated with greater probabilities of mosaic levels ≤20% (OR = 1.326, 95% CI = 1.187–1.481), <50% (OR = 1.425, 95% CI = 1.262–1.608), and ≤80% (OR = 1.351, 95% CI = 1.186–1.539) (p < 0.001). The prediction abilities of TLBMSs were similar for mosaic levels ≤20% (AUC = 0.604, 95% CI = 0.565–0.642), <50% (AUC = 0.634, 95% CI = 0.598–0.671), and ≤80% (AUC = 0.617, 95% CI = 0.576–0.658). In conclusion, detailed evaluation with TL monitoring at the specific time window reveals that redefined blastocyst morphology components and converted numeric TLBMSs are significantly correlated with all of the threshold levels of mosaicism. However, the performance of TLBMSs to differentiate blastocysts with aberrant ploidy risk remains perfectible.

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Section: Discussionsupporting

confidence: 90%

Blastocyst Morphology Based on Uniform Time-Point Assessments is Correlated With Mosaic Levels in Embryos

Chen¹,

Lee

Huang³

et al. 2021

Front. Genet.

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“…There was no significant difference between IVF and ICSI in terms of the fertilization rate, embryo development or the number of euploid embryos. A retrospective study by Deng et al [ 27 ] found that the numbers of IVF–PGT-A and ICSI–PGT-A had similar embryo aneuploidy and mosaic ratios. Conventional IVF fertilization is unlikely to cause serious contamination during PGT-A.…”

Section: Discussionmentioning

confidence: 99%

Non-invasive preimplantation genetic testing for conventional IVF blastocysts

Xie

Zhang

et al. 2022

J Transl Med

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Background Previous studies suggested that non-invasive preimplantation genetic testing (niPGT) for intracytoplasmic sperm injection (ICSI) blastocysts can be used to identify chromosomal ploidy and chromosomal abnormalities. Here, we report the feasibility and performance of niPGT for conventional in vitro fertilization (IVF) blastocysts. Methods This was a prospective observational study. In the preclinical stage, whole genome amplification and NGS were performed using the sperm spent culture medium (SCM). Then, trophectoderm (TE) biopsies and corresponding SCM derived from 27 conventional IVF monopronuclear embryos were collected. In the clinical stage, samples from 25 conventional IVF cycles and 37 ICSI cycles from April 2020–August 2021 were collected for performance evaluation. Results Preclinically, we confirmed failed sperm DNA amplification under the current amplification system. Subsequent niPGT from the 27 monopronuclear blastocysts showed 69.2% concordance with PGT results of corresponding TE biopsies. In the clinical stage, no paternal contamination was observed in any of the 161 SCM samples from conventional IVF. While maternal contamination was observed in 29.8% (48/161) SCM samples, only 2.5% (4/161) samples had a contamination ratio ≥ 50%. Compared with that of TE biopsy, the performances of NiPGT from 161 conventional IVF embryos and 122 ICSI embryos were not significantly different (P > 0.05), with ploidy concordance rates of 75% and 74.6% for IVF and ICSI methods, respectively. Finally, evaluation of the euploid probability of embryos with different types of niPGT results showed prediction probabilities of 82.8%, 77.8%, 62.5%, 50.0%, 40.9% and 18.4% for euploidy, sex-chromosome mosaics only, low-level mosaics, multiple abnormal chromosomes, high-level mosaics and aneuploidy, respectively. Conclusions Our research results preliminarily confirm that the niPGT approach using SCM from conventional IVF has comparable performance with ICSI and might broadening the application scope of niPGT.

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“…In line with these findings, several observational studies have shown similar results in terms of the number of embryos available for biopsy, aneuploidy, and mosaicism rates, as well as similar percentages of complete and incomplete diagnoses with either IVF-only or ICSI-only and split insemination approaches. This was true either for PGT for aneuploidies or for PGT for monogenic diseases (46)(47)(48)(49). Therefore, the available bulk of evidence does not suggest an advantage for ICSI, and we can only conclude that the generalized use of ICSI in PGT cycles was adopted on the basis of a ''fear'' of contamination rather than on the basis of solid scientific evidence.…”

Section: Intracytoplasmic Sperm Injection For Few (Continued)mentioning

confidence: 96%