Blastocyst Morphology Based on Uniform Time-Point Assessments is Correlated With Mosaic Levels in Embryos

Chen, Chien‐Hong; Lee, Chun‐I; Huang, Chun‐Chia; Chen, Hsiu‐Hui; Ho, Shu-Ting; Cheng, En‐Hui; Lin, Pin‐Yao; Chen, Chung-I; Lee, Tsung-Hsien; Lee, Maw‐Sheng

doi:10.3389/fgene.2021.783826

Cited by 8 publications

(12 citation statements)

References 48 publications

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“…Due to the observational nature of retrospective studies, it should not be assumed that there is a causal relationship between the euploidy of the embryo with the status of the polar body; controlled experiments need to be carefully designed before providing clinical recommendations. In this study, we retrospectively evaluated the status of polar body and euploidy of embryo that could be considered for transfer by next-generation sequencing (NGS) analysis among patients undergoing preimplantation genetic testing (PGT) treatment ( Chen et al, 2021 ) between January 2020 and December 2021 at the Reproductive Medicine Center of Tongji Hospital. Only embryo biopsies in fresh cycles were considered to eliminate the effects of vitrification.…”

Section: Methodsmentioning

confidence: 99%

Correlation of the position and status of the polar body from the fertilized oocyte to the euploid status of blastocysts

Yang

Tan²,

Chen³

et al. 2022

Front. Genet.

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Polar bodies are tiny cells that are extruded during oocyte meiosis and are generally considered not essential for embryonic development. Therefore, polar bodies have been widely used as important materials for the preimplantation genetic diagnosis of human embryos. Recent studies have shown that polar bodies mediate embryonic development and that their morphology is related to embryo quality and developmental potential. However, the relationship between the emission of the polar body and embryonic euploidy remains unclear. In this study, a total of 1,360 blastocyst trophectoderm (TE) biopsies were performed, and blastocyst ploidy results were correlated with the state of polar bodies. The results showed that polar body angle size and polar body status are not directly related to whether the blastocysts are euploid, aneuploid, or mosaic (p > 0.05). Therefore, in the process of clinical embryo selection, embryologists should not predict the euploidy of blastocysts based on the state of polar bodies, thus affecting embryo selection.

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Section: Methodsmentioning

confidence: 99%

Correlation of the position and status of the polar body from the fertilized oocyte to the euploid status of blastocysts

Yang

Tan²,

Chen³

et al. 2022

Front. Genet.

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“…We discovered differences in the distribution of embryo morphological classification among the euploid and aneuploid groups. Individual morphological components adopted in blastocyst evaluation (i.e., expansion degrees, inner cell mass grades, and trophectoderm grades) have been independently correlated with euploid/aneuploid status ( 17 , 18 ). Considering the grade of expansion, grade 3 represented the most common value across all groups; however, we found significant differences in this distribution.…”

Section: Discussionmentioning

confidence: 99%

“…Considering the grade of expansion, grade 3 represented the most common value across all groups; however, we found significant differences in this distribution. Chen et al indicated a greater likelihood of euploidy among blastocysts with good-quality inner mass cell or trophectoderm morphology ( 17 ). Similarly, most euploid embryos had grade A inner mass cell morphology, while grade B represented the most observed grade for aneuploidies.…”

Section: Discussionmentioning

confidence: 99%

The embryo mosaicism profile of next-generation sequencing PGT-A in different clinical conditions and their associations

Heiser¹,

Cagnin²,

Souza³

et al. 2023

Front. Reprod. Health

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IntroductionUniform chromosome abnormalities are commonly seen in early pregnancy loss, with analyses of the product of conception suggesting the presence of mosaic autosomal trisomy in ∼10% of cases. Although chromosomal mosaicism occurs in a minority of embryos, their relative commonality and uncertainty regarding associated transfer outcomes have created discussion at both the clinical and research levels, highlighting the need to understand the clinical conditions associated with the incidence of embryo mosaicism.MethodsWe took advantage of a preimplantation genetic testing for aneuploidy (PGT-A) database created from 2019 to 2022 in more than 160 in vitro fertilization (IVF) clinics in Brazil, the second-largest world market for IVF. We carried out descriptive statistical and associative analyses to assess the proportions of mosaicism associated with clinical conditions and reported incidence by chromosome, clinic origin, and biopsy operator.ResultsChromosomal analysis revealed that most mosaic aneuploidies occurred in the last three chromosomes, with 78.06% of cases having only one chromosome affected. Low mosaicism in trisomy represented the most ordinary form, followed by low mosaicism in monosomy. We identified associations between low (negatively-associated) and high mosaicism (positively-associated) and maternal age, indication (male factor and uterus/ovarian factor negatively associated with low and high mosaic, respectively), day of blastocyst development (day five has an overall better outcome), morphology grade (lower quality increased the chances of low and high mosaicism), origin (vitrified oocyte and embryo increased the rates of low and high mosaicism, respectively), and embryo sex (male embryos negatively associated with low mosaic).DiscussionWith these results, we hope to foster an improved understanding of the chromosomal mosaicism linked with distinct clinical conditions and their associations in Brazil.

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“…[ 40 ]. Another retrospective cohort study by Chen et al used multivariate generalized estimating equation analysis to evaluate the correlation of time-lapse-based variables and numeric blastocyst morphological scores with different mosaic levels (≤ 20%, ≤ 50% and ≤ 80%) in 918 biopsied blastocysts and suggested that blastocyst morphokinetic are significantly correlated with the threshold levels of mosaicism[ 28 ]. The correlation between blastocyst morphology and the mosaicism level is unclear.…”

Section: Discussionmentioning

confidence: 99%

The correlation between morphological parameters and the incidence of de novo chromosomal abnormalities in 3238 biopsied blastocysts

Gao

Wei

Zhu

et al. 2023

J Assist Reprod Genet

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Purpose The aim of this study was to determine the relationship between morphological parameters and the incidence of de novo chromosomal abnormalities. Methods This was a retrospective cohort study of 652 patients who underwent 921 cycles with 3238 blastocysts biopsied. The embryo grades were evaluated according to Gardner and Schoolcraft’s system. The incidence of euploidy, whole chromosomal aneuploidy (W-aneuploidy), segmental chromosomal aneuploidy (S-aneuploidy), and mosaicism in trophectoderm (TE) cell biopsies was analyzed. Results The euploidy decreased significantly with maternal age and was positively correlated biopsy day and morphological parameters. The W-aneuploidy increased significantly with maternal age and was negatively correlated biopsy day and morphological parameters. Parental age, TE biopsy day, and morphological parameters were not associated with S-aneuploidy and mosaicism, except that TE grade C blastocysts had significantly higher mosaicism than TE grade A blastocysts. Subanalysis in different female age groups showed that euploidy and W-aneuploidy had a significant correlation with TE biopsy day among women aged ≤ 30 y and 31–35 y, with expansion degree among women aged ≥ 36 y, with ICM grade among women aged ≥ 31 y, and with TE grade among all female age ranges. Conclusion Female age, embryo developmental speed and blastocyst morphological parameters are associated with euploidy and whole chromosomal aneuploidy. The predictive value of these factors varies across female age groups. Parental age, embryo developmental speed, expansion degree, and ICM grade are not associated with the incidence of segmental aneuploidy or mosaicism, but TE grade seemingly has a weak correlation with segmental aneuploidy and mosaicism in embryos.

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Blastocyst Morphology Based on Uniform Time-Point Assessments is Correlated With Mosaic Levels in Embryos

Cited by 8 publications

References 48 publications

Correlation of the position and status of the polar body from the fertilized oocyte to the euploid status of blastocysts

Correlation of the position and status of the polar body from the fertilized oocyte to the euploid status of blastocysts

The embryo mosaicism profile of next-generation sequencing PGT-A in different clinical conditions and their associations

The correlation between morphological parameters and the incidence of de novo chromosomal abnormalities in 3238 biopsied blastocysts

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