“…Four strains, B. afzelii strain PGau from cerebrospinal fluid from a Lyme disease patient in Germany, B. garinii strain HPI isolated from I. persulcatus in Hokkaido, Japan, B. valaisiana strain KRI from a wild rodent, Rattus losea captured in Kinmen island and B. valaisiana strain TMI isolated from a wild rodent, Mus formosanus captured in Taichung (11), were used as antigens for enzyme-linked immunosorbent assay (ELISA). Preparation of antigen and ELISA were performed according to the method described by Magnarelli et al (9). Affinity-purified horseradish peroxidase (HRP)-Iabeled goat anti-mouse IgG-IgM-IgA serum (Kirkegaard & Perry Laboratory, Md., U.S.A) diluted to 1:I,000 was used as a second antibody.…”