Serological Survey for Lyme Disease in Sika Deer (<i>Cervus nippon yesoensis</i>) by Enzyme‐Linked Immunosorbent Assay (ELISA)

Isogai, Emiko; Isogai, Hiroshi; Masuzawa, Toshiyuki; Yanagihara, Yasutake; Sato, Nanao; Hayashi, Shunji; Maki, Toshihide; Mori, Mamoru

doi:10.1111/j.1348-0421.1991.tb01603.x

Cited by 16 publications

(16 citation statements)

References 16 publications

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“…The percentage was higher than 25% in another endemic area of Hokkaido (12). In the study site, sika deer escaped extermination by a hair's breadth.…”

Section: Discussionmentioning

confidence: 95%

“…Wild animals are important as reservoirs or susceptible hosts for B. burgdorferi (1,7,27). From serological surveys, it is clear that various mammalian hosts are infected with B. burgdorferi sensu lato in Japan (2,12,13). B. burgdorferi was isolated from the spleen of Apodemus speciosus (wild rodent) (30) and from a wild fox with systemic infection (11) in the Hokkaido region of Japan.…”

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confidence: 99%

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Borrelia burgdorferi Sensu Lato in an Endemic Environment: Wild Sika Deer (Cervus nippon yesoensis) with Infected Ticks and Antibodies

Isogai

Masuzawa

et al. 1996

Microbiology and Immunology

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Ticks and blood samples were collected from wild sika deer (Cervus nippon yesoensis) during a hunting season (August to October) of 1991 at a selected location in Hokkaido, Japan. Ixodes persulcatus (adult and nymph) and I. ovatus (adult) were the common ticks on sika deer. Spirochetes were detected in the midgut of the ticks by the indirect peroxidase-conjugated antibody staining method and by dark-field microscopy after cultivation. By the reactive pattern of monoclonal antibodies, isolates were considered to belong to Borrelia garinii or B. japonica. In an antibody test, the percentage of seropositive deer was 69.0%. Most of the adult sika deer were positive for antibodies to the spirochetes. There are significant age-dependency in antibody level and seropositive rate. The surveillance of deer should be valuable in monitoring the transmission risk of B. burgdorferi sensu lato in nature.

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“…The percentage was higher than 25% in another endemic area of Hokkaido (12). In the study site, sika deer escaped extermination by a hair's breadth.…”

Section: Discussionmentioning

confidence: 95%

mentioning

confidence: 99%

Borrelia burgdorferi Sensu Lato in an Endemic Environment: Wild Sika Deer (Cervus nippon yesoensis) with Infected Ticks and Antibodies

Isogai

Masuzawa

et al. 1996

Microbiology and Immunology

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“…In Japan, seroepidemiological studies of Lyme borreliosis have been carried out among humans, pets, and domestic animals. However, there is little information available concerning Japanese wild animals, with the exception of a study of wild sika deer (Cervus nippon yesoensis) reported by Isogai et al [6]; because it is difficult to collect samples from wild animals and there are few serological methods adaptable to the wide range of animal species. It was recently reported that protein G reacts to a wide range of animal species' immunoglobulins, and therefore it has been gradually introduced for use in serological assays [1,4,7].…”

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confidence: 99%

“…Our results indicate that Lyme borreliosis is prevalent in wild Japanese serows, and the positive rate (23%) is relatively higher than that in companion and domestic animals. In Hokkaido, Japan, Isogai et al [6] reported that the positive rate of sika deer with Lyme disease was 25.0%; thus, sika deer are assumed to be one important host. Furthermore, there have been many reports involving the serological surveillance of wild animals around the world [2,[9][10][11].…”

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Serological Surveillance of Lyme Borreliosis in Wild Japanese Serows (Capricornis crispus).

Sugiyama

Minamoto

Kinjo

1998

The Journal of Veterinary Medical Science

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ABSTRACT. Through serological surveillance of wild animals by enzyme linked immunosorbent assay with protein G (PG-ELISA), we obtained epidemiological data on Lyme borreliosis in Japanese wild animals. In this study, we carried out serological surveillance for Lyme borreliosis in wild Japanese serows (Capricornis crispus). Forty-six of 200 (23%) serum samples were positive, indicating that Lyme borreliosis is prevalent in wild Japanese serows. This positive rate was relatively higher than that of other animals and was similar/ identical to that in other important hosts worldwide. Our results suggest that Japanese serows may be one of the important hosts of Lyme borreliosis in the central mountainous and forested areas of Japan. -KEY WORDS: Japanese serow, Lyme borreliosis, protein G ELISA.

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“…The microorganism is transmitted by infected ixodid ticks to a broad range of vertebrates, including humans, mice, dogs, and deer (1,3,(11)(12)(13)23). In Japan, it has been reported only in humans (1,11), dogs (13), and Sika deer (12). Human infection in Japan with B. burgdorferi is often asymptomatic (11).…”

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T Cell Response to Borrelia garinii, Borrelia afzelii, and Borrelia japonica in Various Congenic Mouse Strains

Ishii

Isogai

et al. 1995

Microbiology and Immunology

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The infectivity and T cell response to Borrelia garinii SIKA2, Borrelia afzelii BFOX, and Borrelia japonica 0612, the organisms that cause Lyme disease in Japan, were examined in various inbred and congenic strains of mice. Infectivity differed among the species: B. garinii SIKA2 and B. afzelii BFOX were each able to infect 90% to 100% of C3H/He mice; B. japonica 0612 was able to infect only 20% of C3H/He mice. The pattern of infectivity to various inbred and congenic strains of mice may influence the pathogenicity of the organism and the clinical signs of Lyme disease. Cross-reactivity between Borrelia antigens was observed, but there was no cross-reactivity between Borrelia antigens and Leptospira antigens. We evaluated the genetic control of the delayed-type hypersensitivity (DTH) reaction in the form of footpad swelling produced by Borrelia antigens using viable or sonicated bacteria as sensitization. Differences in strains of mice infected by viable antigen were observed. However, all strains of mice showed a strong DTH reaction using sonicated antigens without genetic background. A DTH reaction in the form of footpad swelling did not appear to be associated with genetic background. The footpad reaction was mediated by CD4+8-and Ia-T cells, as revealed by in vitro monoclonal antibody treatment. However, CD8+ T cells did not suppress footpad swelling. These results indicate that many antigenic epitopes of the Borrelia spirochete can stimulate the DTH reaction.

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Serological Survey for Lyme Disease in Sika Deer (Cervus nippon yesoensis) by Enzyme‐Linked Immunosorbent Assay (ELISA)

Abstract: Seventy-six wild sika deer (Cervus nippon _yesoensis) from areas endemic for

Cited by 16 publications

References 16 publications

Borrelia burgdorferi Sensu Lato in an Endemic Environment: Wild Sika Deer (Cervus nippon yesoensis) with Infected Ticks and Antibodies

Borrelia burgdorferi Sensu Lato in an Endemic Environment: Wild Sika Deer (Cervus nippon yesoensis) with Infected Ticks and Antibodies

Serological Surveillance of Lyme Borreliosis in Wild Japanese Serows (Capricornis crispus).

T Cell Response to Borrelia garinii, Borrelia afzelii, and Borrelia japonica in Various Congenic Mouse Strains

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