Comparison of Albicans ID2® agar plate with the germ tube for presumptive identification of Candida albicans

Cárdenes, C.D.; Carrillo, Alfonso J.; Arias, Á.; Rodríguez-Álvarez, Cristobalina; Torres-Lana, A.; Sierra, A.; Arévalo, M.P.

doi:10.1016/s0732-8893(01)00346-7

Cited by 19 publications

(18 citation statements)

References 26 publications

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“…Esto indica que dicho esquema diagnóstico secuencial es comparable en capacidad de discriminación al uso de cualquiera de los medios cromógenos habituales, pero con un aumento de la especificidad (5,6,8,29). Era de esperar la ausencia de falsos positivos ya que el aislamiento tendría que presentar el mismo error en las dos pruebas.…”

Section: Discussionunclassified

Identificación de Candida glabrata y otras especies comunes del género Candida mediante el uso secuencial del medio de cultivo cromógeno y la prueba del tubo germinal

Hernández-Botero

Pérez-Cárdenas

2015

iatreia

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RESUMENIntrodución: se han usado agar cromógeno (AC) y la prueba del tubo germinal (PTG) para identificar C. albicans. Sin embargo, ninguna de estas dos pruebas por separado permite la identificación de C. glabrata.Objetivo: analizar la eficacia diagnóstica del uso secuencial del AC y la PTG para identificar las especies más comunes de Candida.Métodos: se identificaron 436 aislamientos usando el AC y luego la PTG; se utilizaron las pruebas bioquímicas como estándar de oro, y se determinaron la sensibilidad y especificidad del esquema secuencial con sus intervalos de confianza.Resultados: el uso en serie del AC y la PTG tuvo sensibilidad del 97,9 % (IC95 %: 96,0-99,9) para identificar C. albicans/dubliniensis y del 90,9 % (IC95 %: 84,0-97,8) para identificar C. tropicalis, con especificidad del 100 % para ambas especies. El mismo esquema permitió identificar C. glabrata con sensibilidad del 92,5 % (IC95 %: 86,2-98,8) y especificidad del 96,6 % (IC95 %: 95,0-98,6), y el complejo C. parapsilosis con especificidad del 96,3 % (IC95 %: 94,5-98,1).Conclusiones: el esquema propuesto permite la identificación de C. albicans/dubliniensis, C. tropicalis y C. glabrata con sensibilidad y especificidad adecuadas, y podría ser útil en entornos clínicos de bajos recursos.

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Section: Discussionunclassified

Identificación de Candida glabrata y otras especies comunes del género Candida mediante el uso secuencial del medio de cultivo cromógeno y la prueba del tubo germinal

Hernández-Botero

Pérez-Cárdenas

2015

iatreia

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“…On the other hand, Cárdenes et al. [18] highlighted the usefulness of methods based on hexosaminidase substrates for the detection and rapid identification of C. albicans . Proper presumptive identification of the studied strains using the CHROMagar Candida ® medium was confirmed by the API 20C AUX test.…”

Section: Discussionmentioning

confidence: 99%

“…CHROMagar ® Candida medium (Becton Dickenson, Sparks, MD, USA), containing a mixture of chromogenic substrates, was used for presumptive identification and assessment of beta‐ N ‐acetylhexosaminidase (HexNAcase) production 12,18 . A single yeast colony grown on Sabouraud agar plate (incubated at 30 °C for 48 h) was streaked (replicated) onto the CHROMagar ® Candida medium and incubated for 48 h at 37 °C under aerobic conditions.…”

Section: Methodsmentioning

confidence: 99%

Discrimination between the enzymatic activities of Candida albicans pleomorphic forms determined using the api® ZYM test

Staniszewska

Rabczenko

Kurzątkowski

2011

Mycoses

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Enzymatic activity profiles for two morphotypes of 37 Candida albicans clinical isolates were compared. Yeast and hyphal forms were grown using yeast extract-peptone-glucose broth or undiluted human serum, respectively. Both morphotypes were documented under scanning electron microscopy. The api(®) ZYM (BioMérieux, France) test was used to evaluate the enzymatic activity profiles for particular pleomorphic forms. None of the examined enzymatic activities showed good agreement (kappa, κ > 0.80) for the two morphotypes of the tested strains. Only leucine arylamidase activity in blastoconidia and hyphae of 35 out of 37 strains appeared to be in significant agreement (κ = 0.770). This phenomenon should be explored further for clinical benefits. For morphotypes of all tested strains, activity profiles of 11 hydrolytic enzymes demonstrated weak agreement (κ = 0.044-0.197). Moreover, satisfactory (κ = 0.218-0.348) and moderate agreement (κ = 0.413-0.479) were noted for enzymatic activity values of five and two enzymes, respectively. The distinct differences in activity profiles of hydrolytic enzymes between hyphae and blastoconidia is suggested to be related to the specific roles of these two morphotypes in particular steps of pathogenesis. Moreover, both morphotypes should be examined by strain biotyping methods. Beta-N-hexosaminidase (HexNAcase) activity assessed by the api(®) ZYM test and on CHROMagar Candida(®) medium (Becton Dickinson, USA) is also discussed.

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“…Albicans ID2 ® chromogenic medium (bioMérieux, Marcy l'Etoile, France) has been developed and marketed for the identification of C. albicans (blue colonies). This assay is based on a chromogenic indolyl glucosaminide substrate, which is hydrolyzed by C. albicans to give a turquoise or blue color (4,12). The purpose of this study was to evaluate the accuracy of Albicans ID2 ® plates to identify C. albicans strains among yeasts different species with clinical relevance.…”

mentioning

confidence: 99%

“…strains (78.6%) and 2 out 5 C. rugosa (40%) strains. Cárdenes et al (4) reported misidentification of yeast by using Candida ID2 ® , after testing C. tropicalis (2 out 69 or 2.8%), C. parapsilosis (3 out 76 or 3.9%) and C. glabrata (2 out 47 or 4%) strains. In our study, the identification of C. albicans using Candida ID2…”

mentioning

confidence: 99%

Performance of the Albicans ID2<FONT FACE=Symbol>Ò</FONT> chromogenic medium for rapid identification of Candida albicans

Godoy-Martínez

Azevedo

Reis

et al. 2006

Braz. J. Microbiol.

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The aim of our study was to evaluate the accuracy of the chromogenic media Albicans ID2 ® (bioMérieux, France) for the identification of Candida albicans among 330 yeast strains. All C. albicans (100) and C. dubliniensis (20) strains exhibited blue color when cultured on Albicans ID2 ® . However, the blue color was also exhibited by cultures of C. rugosa (30/30) and C. tropicalis (3/50) isolates.

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Comparison of Albicans ID2® agar plate with the germ tube for presumptive identification of Candida albicans

Cited by 19 publications

References 26 publications

Identificación de Candida glabrata y otras especies comunes del género Candida mediante el uso secuencial del medio de cultivo cromógeno y la prueba del tubo germinal

Identificación de Candida glabrata y otras especies comunes del género Candida mediante el uso secuencial del medio de cultivo cromógeno y la prueba del tubo germinal

Discrimination between the enzymatic activities of Candida albicans pleomorphic forms determined using the api® ZYM test

Performance of the Albicans ID2<FONT FACE=Symbol>Ò</FONT> chromogenic medium for rapid identification of Candida albicans

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