Comparison of Ah Receptor-Mediated Luciferase and Ethoxyresorufin-O-deethylase Induction in H4IIE Cells: Implications for Their Use as Bioanalytical Tools for the Detection of Polyhalogenated Aromatic Hydrocarbons

Sanderson, J. Thomas; Aarts, J.M.M.J.G.; Brouwer, Abraham; Froese, Kenneth L.; Denison, Michael S.; Giesy, John P.

doi:10.1006/taap.1996.0086

Cited by 234 publications

(186 citation statements)

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“…The analysis used H4IIE-luc-specific TEFs to calculate TEQ (e.g. Sanderson et al, 1996;Khim et al, 2001;Koh et al, 2004) based on concentrations of PCDD, PCDF and AhR-active PCB congeners to all the dioxin-like activity in the sediment samples.…”

Section: Chemical Analysismentioning

confidence: 99%

“…The H4IIE-luciferase induction assay is an in vitro technique that offers a more cost effective alternative for the identification of AhR-active compounds (Sanderson et al, 1996). The assay is also referred to as the chemical activated luciferase gene expression (CALUX) system based on rat hepatoma cells stably transfected with an AhR-controlled luciferase reporter gene construct (Murk et al, 1996;Sanderson et al, 1996;Giesy et al, 2002). Luciferase induction potential is assessed by comparing the response to the sample with the response to TCDD (Villeneuve et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…This bioanalytical assay has been used to detect and determine the toxicity of complex mixtures of dioxin-like compounds in extracts of environmental samples such as soil, water, food and biota (Villeneuve et al, 2000;Behnisch et al, 2001), but is particularly useful for the rapid screening of total concentrations of TCDD-EQ in sediments (Khim et al, 2001;Hilscherova et al, 2001). The H4IIE-luc cell line was developed at Michigan State University (Sanderson et al, 1996). The transfected cells contain an AhR element which controls the luciferase reporter gene.…”

Section: Introductionmentioning

confidence: 99%

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AhR-active compounds in sediments of the Haihe and Dagu Rivers, China

Song

Jiang

et al. 2006

Chemosphere

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Total concentrations of compounds that can cause activation of the aryl hydrocarbon receptor (AhR) in extracts of river sediments from various locations in the Haihe and Dagu Rivers, Tianjin, China were determined by use of the in vitro H4IIE-luc cell line. AhR-active compounds were isolated from sediments by Soxhlet extraction, and the crude extracts were fractionated using a Florisil column into three Fractions. The response of samples was compared to the TCDD standard and expressed as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) equivalents (TCDD-EQs). Significant dioxin-like activity was observed in each sample. The TCDD-EQs in crude extracts of sediments (SCEs), as determined with the bioassay were 2-4 times greater than the sum of the TCDD-EQs of the eluent from fractions separated with a Florisil column. The results also showed that Fractions 2 and 3 contained most of the AhR-mediated activity. The results obtained by using the bioassay were compared with those of previous measurements of the concentrations of polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) and the structurally-similar AhR-active polychlorinated biphenyls (PCBs). It was determined that sediments from the Dagu River contained greater concentrations of TCDD-EQ than did sediments from the Haihe River except at Jingangqiao (location R3), which is associated with industrial activities in the adjacent densely populated area. The concentrations of TCDD-EQs, based on the EC 20 and the relative potency ranges, of SCEs ranged from 330 to 930 and 1200 to 13 900 pg TCDD-EQ g À1 dry wt in Haihe and Dagu Rivers, respectively.

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Section: Chemical Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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AhR-active compounds in sediments of the Haihe and Dagu Rivers, China

Song

Jiang

et al. 2006

Chemosphere

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“…Another possible explanation of our observations is that the test was performed for water only and most of the hydrophobic chemical should be concentrated in the sludge or suspended matter, which was removed from the sedimentation processes. Also, dioxin-like chemicals are found in the environment as complex mixtures of both AhR-active and AhR-inactive congeners, and interactions between active and inactive congeners may complicate the effects on in vitro bioassay responses (Sanderson et al 1996). It was illustrated that the EROD assay may underestimate the potential toxicity of chemicals, such as PAHs and other AhR ligands that are readily metabolized (Bols et al1997;Whyte et al 2004).…”

Section: Dioxin-like Chemicalsmentioning

confidence: 99%

Assessing the Detoxication Efficiencies of Wastewater Treatment Processes Using a Battery of Bioassays/Biomarkers

Wang

2005

Arch Environ Contam Toxicol

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Abstract. A battery of in vitro bioassays, including a Neutral Red (NR) assay using MCF-7 cells for predicting cytotoxic chemicals, an ethoxy resorufin-O-deethylase (EROD) activity assay using H4IIE cells to check for dioxin-like chemicals, and a recombinant gene yeast assay for screening estrogenic chemicals, was conducted to assess the removal efficiencies of trace toxic chemicals by different treatment processes in the waste water treatment plant (WWTP). The effluents were extracted by solid phase extraction (SPE) and were fractionated into three fractions based on polarities. The battery of bioassays was performed for each fraction. In the battery, the toxicities of the effluents were described according to their modes of actions (MOA) or biomarkers and the properties of the toxic chemicals were categorized by their polarities and MOAs. The proposed procedure could be used as a tool to diagnose the toxic characteristics of the complicate mixture. The results showed that cytotoxic, dioxin-like and estrogenic chemicals could be detected in all samples. In the influent, cytotoxic and dioxin-like chemicals were mainly in polar fraction and estrogenic chemicals were in non-polar and moderate-polar fractions. The secondary treatment (active sludge) could remove a small amount of these toxicants. Among different types of advanced treatments, flocculation was good enough to remove most of the cytotoxic chemicals and a combination of flocculation, ozone oxidation, and post-biological treatment could eliminate most of the dioxin-like and estrogenic chemicals.

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“…The cells employed in the present study, H4IIE-luc, were rat hepatoma cells that were stably transfected with a luciferase reporter gene (Sanderson et al, 1996). The procedures for the in vitro bioassay were similar to those described in Khim et al (2000Khim et al ( , 2001.…”

Section: H4iie-luc Cell Bioassaymentioning

confidence: 99%