Comparison of adhesin genes expression among Klebsiella oxytoca ESBL-non-producers in planktonic and biofilm mode of growth, and imipenem sublethal exposure

Alkhudhairy, Miaad K.; Al-Shadeedi, Shahrazad M.; Mahmood, Suhad Saad; Albustan, Sana A.; Ghasemian, Abdolmajid

doi:10.1016/j.micpath.2019.103558

Cited by 24 publications

(11 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The strains were classified upon Donelli et al 2012 as non-biofilm producers, weak biofilm producers, moderate biofilm former and strong biofilm producers. In addition, the standard strain K. pneumoniae ATCC 13,833 and LB broth were considered as a positive and negative control, respectively (Alkhudhairy et al 2019 ).…”

Section: Methodsmentioning

confidence: 99%

Antibiotic resistance, virulence-associated genes analysis and molecular typing of Klebsiella pneumoniae strains recovered from clinical samples

Mirzaie

Ranjbar

2021

AMB Expr

View full text Add to dashboard Cite

Klebsiella pneumoniae is a multidrug-resistant (MDR) opportunistic pathogen that causes nosocomial infections. Virulence analysis and molecular typing as powerful approaches can provide relevant information on K. pneumoniae infection. In the current study, antibiotic resistance, virulence-associated genes analysis, as well as molecular typing of K. pneumoniae strains were investigated. Out of 505 clinical samples collected from hospitalized patients, 100 K. pneumoniae strains were isolated by standard microbiological methods and subjected to the phenotypic and genotyping analysis. The highest prevalence of resistance was observed against ciprofloxacin (75%), trimethoprim–sulfamethoxazole (73%) and nitrofurantoin (68%). Virulence associated genes including entB, traT, ybts, magA, iucC, htrA and rmpA were found in 80%, 62%, 75%, 5%, 30%, 72% and 48%, of the isolates, respectively. The prevalence of biofilm-associated genes including mrkA, fimH, and mrkD were equally 88% for all tested isolates. Moreover, the efflux pump genes including AcrAB, TolC and mdtK were observed in 41 (41%), 33 (33%) and 26 (26%) of the strains respectively. A significant statistical association was observed between MDR strains and high expression of efflux pump and biofilm genes. The K. pneumoniae strains were differentiated into 11 different genetic patterns using the repetitive element sequence-based PCR (rep-PCR) technique. High prevalence of resistance, presence of various virulence factors, high level of efflux pump, and biofilm gene expression in diverse clones of K. pneumoniae strains pose an important health issue in clinical settings.

show abstract

Section: Methodsmentioning

confidence: 99%

Antibiotic resistance, virulence-associated genes analysis and molecular typing of Klebsiella pneumoniae strains recovered from clinical samples

Mirzaie

Ranjbar

2021

AMB Expr

View full text Add to dashboard Cite

show abstract

“…Those that have been performed show that it is important clinically, as a nosocomial pathogen able to cause blood borne infection in immunocompromised patients, and severe, possibly fatal gut damage in preterm infants [ 8 – 11 ]. Apart from their incapacity to penetrate bacterial biofilms [ 20 ], antibiotics may increase colonisation and biofilm formation by Klebsiella spp [ 18 ]. Recent reports of the purification of enzymes capable of interfering with quorum sensing (involved in cell-cell communication in bacterial biofilms) offer new prospects for disruption/prevention of biofilm formation [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Novel Drexlerviridae bacteriophage KMI8 with specific lytic activity against Klebsiella michiganensis and its biofilms

Kabwe

Chan

et al. 2021

PLoS ONE

View full text Add to dashboard Cite

The bacterial genus Klebsiella includes the closely related species K. michiganensis, K. oxytoca and K. pneumoniae, which are capable of causing severe disease in humans. In this report we describe the isolation, genomic and functional characterisation of the lytic bacteriophage KMI8 specific for K. michiganensis. KMI8 belongs to the family Drexlerviridae, and has a novel genome which shares very little homology (71.89% identity over a query cover of only 8%) with that of its closest related bacteriophages (Klebsiella bacteriophage LF20 (MW417503.1); Klebsiella bacteriophage 066039 (MW042802.1). KMI8, which possess a putative endosialidase (depolymerase) enzyme, was shown to be capable of degrading mono-biofilms of a strain of K. michiganensis that carried the polysaccharide capsule KL70 locus. This is the first report of a lytic bacteriophage for K. michiganensis, which is capable of breaking down a biofilm of this species.

show abstract

“…Other common fimbrial adhesins are the P-type, which bind to glycolipid receptors and present as their main component the PapA, adapter subunits, and terminal PapG. In addition to these, type 3 are also highlighted, which bind to the target substrate using the MrkD subunit associated with the fimbrial stem that constitutes the MrkA protein (Zamani and Salehzadeh 2018;Alkhudhairy et al 2019). In several species of the Enterobacteriaceae family, genes encoding fimbriae are identified, as well as in bacteria from other families such as A. baumannii (Mohajeri et al 2016;Tavakol et al 2018) and P. aeruginosa, which synthesizes type IV pili to aid in surface adhesion (Laverty et al 2014).…”

Section: Fimbrial Adhesinsmentioning

confidence: 99%

Virulence factors of human pathogens: an always-needed approach / Fatores de virulência de patógenos humanos: uma abordagem sempre necessária

Silva¹,

Silva²,

Sá³

et al. 2022

Braz. J. Hea. Rev.

View full text Add to dashboard Cite

Hospital infections caused by resistant bacteria are a worldwide public health problem that mainly affects immunocompromised patients. These infections are mainly caused by inadequate antibiotic prescriptions, self-medication and long hospital stays. The World Health Organization mentions a list of resistant bacteria, especially Gram-negative ones considered as priorities for research, discovery and development of new drugs. These pathogens to survive and propagate use a varied set of strategies known as virulence factors. The present study corresponds to a review of the state-of-the-art related to main factors associated with pathogenicity in clinical important Gram-positive and Gram-negative bacteria. The virulence factors, alone or together, guarantee important defenses to the host organism's immune system, as well as adverse external conditions. Consequently, there is an increase in morbidity and mortality rates in hospital environments. Thus, effective microbial control measures are needed, especially in health institutions; also, the encouragement of new therapeutic approaches that target the main virulence factors.

show abstract

Comparison of adhesin genes expression among Klebsiella oxytoca ESBL-non-producers in planktonic and biofilm mode of growth, and imipenem sublethal exposure

Cited by 24 publications

References 12 publications

Antibiotic resistance, virulence-associated genes analysis and molecular typing of Klebsiella pneumoniae strains recovered from clinical samples

Antibiotic resistance, virulence-associated genes analysis and molecular typing of Klebsiella pneumoniae strains recovered from clinical samples

Novel Drexlerviridae bacteriophage KMI8 with specific lytic activity against Klebsiella michiganensis and its biofilms

Virulence factors of human pathogens: an always-needed approach / Fatores de virulência de patógenos humanos: uma abordagem sempre necessária

Contact Info

Product

Resources

About