Comparison of a one-step and a two-step polymerase chain reaction with degenerate general primers in a population-based study of human papillomavirus infection in young Swedish women

To characterize the risk of malignant progression of cervical epithelial lesions associated with human papillomavirus (HPV) types of yet unknown oncogenic potential the prevalences of these HPVs in different cervical epithelial lesions of 809 patients were determined. HPV types 53, 73, and CP8304 were detected in genital specimens of 16, 22, and 12 of the patients, respectively. The ratio of prevalence in high grade dysplastic lesions or cancers and low grade dysplastic lesions or normal specimens was calculated and compared to corresponding values of well known high-risk (HR) and low-risk (LR) HPVs. For HPV 6, 11, 16, 18, 35, and 73 a ratio of 0.1, 0.2, 5.9, 6.5, 2.5, and 2.4, respectively, was calculated. The ratios of HPV53 and CP8304 were less than 1. Moreover, in contrast to HPV73, these viruses have never been detected in cancer specimens. Thus, HPV53 and CP8304 infections are probably not associated with a high risk of carcinogenesis, while HPV73 could be another HR-HPV type.

Section: Discussionmentioning

confidence: 99%

Distribution of HPV 53, HPV 73 and CP8304 in genital epithelial lesions with different grades of dysplasia

Meyer¹,

Arndt²,

Beckmann³

et al. 2001

“…To efficiently identify HPV 16 variants in low-copy number HPV DNA, nested PCR was performed. The conditions used were as previously described (14,15) .…”

Section: Detecting Hpv 16 Infection By Polymerase Chain Reactionmentioning

confidence: 99%

Polymorphism in theE6gene of human papillomavirus type 16 in the cervical tissues of Korean women

Kang¹,

Jeon²,

Kim³

et al. 2005

The aim of this study was to identify sequence variants in the HPV 16 E6 gene in Korean women and to examine the possible association between these sequence variants and cervical cancer development. We examined the HPV 16 DNA of 215 patients with no cervical disease (NCD) (n = 105) or with cervical neoplasia (n = 110) [cervical intraepithelial neoplasia (CIN), n = 61; invasive cervical carcinoma (ICC), n = 49] using the nested polymerase chain reaction (PCR) and PCR-directed sequencing methods. Fifty-four (NCD, n = 10; CIN, n = 17; ICC, n = 27) of the 215 samples contained HPV 16 E6 DNA, but only two (7.4%) of 27 ICC samples had prototype sequences. The most frequently found variation was D25E (in NCD, n = 8, 80%; in CIN, n = 9, 52.9%; in ICC, n = 23, 85.2%). This is a rare variation in western countries. No significance difference was found between the frequencies of D25E variation in cancerous and non-cancerous lesions. Among the 11 kinds of variants identified, four variants were novel and have been registered with GenBank. This study demonstrates that the D25 variant is the most prevalent E6 genomic variant type in Korean population. However, it was not found to be associated with an increased risk of ICC.

“…They conclude that the use of the MY/GP nested PCR increased significantly the positivity rate of HPV DNA detection and should be used for samples with a low copy number of HPV DNA. Evander et al (31) compared the results obtained with MY, GP, and nested MY/GP primers. There were 56.5% specimens negative with MY and positive only with GP primers.…”

Section: Pcr Versus Morphological Criteriamentioning

confidence: 99%

Correlation between histological criteria and human papillomavirus presence based on PCR assay in cervical biopsies

Sálvia¹,

Bonesso-Sabadini²,

Tagliarini³

et al. 2004