Comparison of 2 chromogenic media for the detection of extended-spectrum β-lactamase producing Enterobacteriaceae stool carriage in nursing home residents

Blane, Beth; Brodrick, Hayley J.; Gouliouris, Theodore; Ambridge, Kirsty E; Kidney, Angela D; Ludden, Catherine; Limmathurotsakul, Direk; Török, M. Estée; Peacock, Sharon J.

doi:10.1016/j.diagmicrobio.2015.11.008

Cited by 18 publications

(15 citation statements)

References 10 publications

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“…The sensitivity of Brilliance ESBL agar was higher than that observed in this study but vice versa. Our study showed increased sensitivity (97.8%) at 48 h on Brilliance ESBL agar, this correlates with Biane et al [28] study in Cambridge shire. Biane et al [28] reported an increase in sensitivity of Brilliance ESBL agar from 59% to 68% after 48 h incubation time.…”

Section: Fig 3 Culture Plate Of Clinical Isolate Of Escherichia Coli From Urine Showing Distinctive Blue Colony Colouration On Brilliancesupporting

confidence: 93%

Evaluation of a Chromogenic Medium for the Detection of ESBL with Comparison to Double Disk Synergy Test

Ezeanya

Agbakoba

Ejike

et al. 2017

BJMMR

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Background: Extended Spectrum Beta Lactamase (ESBL) producing bacterial strains are the major causes of nosocomial and community-acquired infections worldwide. The aim of the study was to evaluate the effectiveness of Brilliance ESBL Agar (BEA) (a chromogenic culture medium) for the detection of ESBL in comparison with Double Disc Synergy Test (DDST) and confirm results from both methods by Single-plex Polymerase Chain Reaction (PCR) as gold standard. Materials and Methods: A total of 75 clinical isolates of Escherichia coli were screened for ESBL production using BEA & DDST from various clinical specimens. The antibiotic susceptibility testing Original Research Article

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Section: Fig 3 Culture Plate Of Clinical Isolate Of Escherichia Coli From Urine Showing Distinctive Blue Colony Colouration On Brilliancesupporting

confidence: 93%

Evaluation of a Chromogenic Medium for the Detection of ESBL with Comparison to Double Disk Synergy Test

Ezeanya

Agbakoba

Ejike

et al. 2017

BJMMR

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“…(), we used a semi‐selective enrichment step prior to plating on selective ESBL agar, which may have affected the recovery rate. The difference in the performance of semi‐selective (using broth selecting for Gram negative rods) and selective enrichment (i.e., broth additionally containing a third‐generation cephalosporin) remains to be elucidated and comparative data are limited (Blane et al., ; Jazmati, Hein, & Hamprecht, ; Kluytmans‐Van Den Bergh et al., ).…”

Section: Antimicrobial Resistant Escherichia Coli Obtained By Nonselementioning

confidence: 99%

Antimicrobial resistant and extended‐spectrum β‐lactamase producing Escherichia coli in common wild bird species in Switzerland

et al. 2019

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A total of 294 fecal swabs from 294 wild birds in Switzerland were cultivated for antimicrobial resistant (AMR) Escherichia coli. Samples were also subcultivated to detect E. coli with extended‐spectrum β‐lactamases (ESBL), carbapenemases, and plasmid‐mediated aminoglycoside or colistin resistance, respectively. Samples from 17 (5.8%) of the birds contained 19 AMR E. coli, whereof 26.3% were multidrug resistant. Five (1.7%) ESBL‐producing E. coli were detected. The isolates harbored bla CTX‐M‐1 (two isolated from carrion crows and from one great spotted woodpecker, respectively), bla CTX‐M‐15 (originating from a grey heron), bla CTX‐M‐55 (from a carrion crow), and bla CTX‐M‐65 (from a common blackbird). Phylogenetic analysis assigned three isolates to commensal phylogroups A and B1, one to extraintestinal pathogenic group B2, and one to phylogroup F. Multilocus sequence typing identified sequence types (STs) that have been found previously in ESBL E. coli in wild birds (ST58, ST205, ST540). One isolate harboring bla CTX‐M‐55 was assigned to the recently emerged fluoroquinolone‐resistant, extraintestinal pathogenic E. coli clone ST1193. Wild birds have the potential to disperse AMR, including clinically important resistance genes, from anthropogenic‐influenced habitats to diverse areas, including vulnerable natural environments such as surface waters or mountain regions.

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“…Likewise, Jazmati et al (2017) showed the added value of an enrichment step for the screening of broad-spectrum cephalosporins-resistant Enterobacterales. In another study, an enrichment step was shown to increase the sensitivity of detection of ESBL-E from 75% to 97% in stool samples (Blane et al 2016).…”

Section: Resultsmentioning

confidence: 99%

“…Aliquots of 100 μL of the stool suspension spiked with ESBL-E were inoculated a) directly onto chromID ESBL agar (bioMérieux), b) into 5 mL of nonselective TS broth, or c) into 5 mL of TS broth with cefotaxime (Acros Organics, Chemie Brunschwig AG, Switzerland) at 0.1 or 0.5 μg/mL (Blane et al, 2016) . Aliquot of 100 μL of the fecal suspension without the addition of ESBL-E directly spread onto chromID ESBL agar (bioMérieux) was used as a negative control.…”

Section: Spiking Experimentsmentioning

confidence: 99%

Optimal detection of extended-spectrum β-lactamase producers, carbapenemase producers, polymyxin-resistant Enterobacterales, and vancomycin-resistant enterococci from stools

Sadek

Poirel

Nordmann

2020

Diagnostic Microbiology and Infectious Disease

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This study compared the impact of different approaches, namely, nonenrichment, nonselective enrichment, and selective (antibiotic-containing) enrichment steps, for detecting extended-spectrum β-lactamase producing Enterobacterales (ESBL-E), carbapenemase-producing Enterobacterales (CPE), polymyxin-resistant Enterobacterales (PMR-E), and vancomycin-resistant enterococci (VRE) from spiked stools. The use of a nonselective 18-h enrichment broth culture significantly improved the recovery rate of all types of resistant bacteria after their plating onto selective media. In addition, the detection of ESBL-E, CPE, PMR-E, and VRE was further improved when using an enrichment step using antibiotic-supplemented broths respectively supplemented with cefotaxime (0.1 μg/mL), ertapenem (0.1 μg/mL), colistin (0.5 μg/mL), and vancomycin (1 μg/mL). Therefore, we showed here that a screening strategy based on a selective broth enrichment step significantly contributes to an increased rate of detection of multidrug-resistant bacteria, which may be crucial in term of improvement of infection control.

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Comparison of 2 chromogenic media for the detection of extended-spectrum β-lactamase producing Enterobacteriaceae stool carriage in nursing home residents

Cited by 18 publications

References 10 publications

Evaluation of a Chromogenic Medium for the Detection of ESBL with Comparison to Double Disk Synergy Test

Evaluation of a Chromogenic Medium for the Detection of ESBL with Comparison to Double Disk Synergy Test

Antimicrobial resistant and extended‐spectrum β‐lactamase producing Escherichia coli in common wild bird species in Switzerland

Optimal detection of extended-spectrum β-lactamase producers, carbapenemase producers, polymyxin-resistant Enterobacterales, and vancomycin-resistant enterococci from stools

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