Background: Extended Spectrum Beta Lactamase (ESBL) producing bacterial strains are the major causes of nosocomial and community-acquired infections worldwide. The aim of the study was to evaluate the effectiveness of Brilliance ESBL Agar (BEA) (a chromogenic culture medium) for the detection of ESBL in comparison with Double Disc Synergy Test (DDST) and confirm results from both methods by Single-plex Polymerase Chain Reaction (PCR) as gold standard. Materials and Methods: A total of 75 clinical isolates of Escherichia coli were screened for ESBL production using BEA & DDST from various clinical specimens. The antibiotic susceptibility testing Original Research Article

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This study was aimed at determining the level of Listeria contaminants and subsequently detects some resistance genes among the isolated Listeria species from lettuce sold in some vegetable markets in Benin City, Nigeria. Twenty-four lettuce samples were purchased from three vegetable markets in Benin City, Nigeria and examined using standard microbiological methods. Microbial characterization revealed Listeria monocytogenes and L. grayi as the predominant species isolated. Plate count analysis on Listeria selective agar revealed that lettuce sold in Oba market and Forestry market had 2 2 the highest and lowest mean count of Listeria species 224.00 x 10 CFU/g and 83.00 x 10 CFU/g respectively. Most (63.75%) of the Listeria species isolates were found to be susceptible to Ofloxacin (5 µg), Ciprofloxacin (10 µg), Streptomycin (10 µg), Gentamycin (10 µg), Pefloxacin (5 µg) whereas species harbouring tetracycline (65%) and erythromycin (60%) resistant genes. The study provides an evidence of the colonization of Listeria species in lettuce sold in Benin City which may pose serious public health threat to the populace.

Background: Antimicrobial resistance among Candida species is an intense public health concern. The aim of the study was to determine the antifungal susceptibility pattern and test for cure of Candida species among women of child bearing age who visited the General Hospital Onitsha, Nigeria with symptoms suggestive of Vulvovaginal Candidiasis (VVC).