Comparison Between Total IgG, C1q, and C3d Single Antigen Bead Assays in Detecting Class I Complement-Binding Anti-HLA Antibodies

Gonzales, Manuel A. Moreno; Mitema, Donald; Smith, Byron H.; Schinstock, Carrie A.; Stegall, Mark D.; Wakefield, Laurie L.; Henderson, Nicole; DeGoey, Steven R.; Kreuter, Justin D.; Gandhi, Manish J.

doi:10.1016/j.transproceed.2017.09.040

Cited by 12 publications

(6 citation statements)

References 16 publications

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“…DSA characterization by SAB assays has enhanced donor selection, and our observation is like earlier studies. [ 34 ] LXM allows us to perform a real crossmatch using donor lysate, which is easier to store than living cells for post-transplantation studies. On the other hand, SAB assays allow us to take a VXM by predicting reactions against HLA specificities.…”

Section: Discussionmentioning

confidence: 99%

Donor-specific antibody detection by single-antigen bead assay for renal transplantation: A 2-year experience from South India

Neeraja,

Kesireddy,

Kumar

et al. 2023

Indian J Nephrol

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Introduction: Recipient sensitization against donor human leukocyte antigens (HLA) plays a key role in transplant rejection, and this risk is best minimized by efficient pre transplant antibody detection. Determination of antibody specificity with the highest sensitivity and degree of resolution to the allelic antigen level is achieved by using single-antigen bead (SAB) assay. Methods: This study evaluated the correlation of Luminex cross match (LXM) with SAB assay for detection of donor-specific antibodies (DSA). A total of 2075 renal transplant patients were screened for the presence of DSA by LXM, complement-dependent cytotoxicity (CDC) cross match, and 125 patients for SAB from January 2018 to December 2019. Results: There was a male preponderance among recipients ( P < 0.0001), and the most affected age group was 21–40 years. HLA typing was done in 550/2075 by DNA PCR-reverse sequence-specific oligonucleotide probes (SSOP) method. HLA DSA by LXM was detected in 16.3% of recipients (338/2075). Majority 180/338 (53.2%) of the patients were class II DSA positive, ( P < 0.0001). Among the class II DSA positive patients, 20/180 (11.1%) samples gave false-positive results by LXM. SAB for class I and class II HLA IgG antibodies was done in 125/338 renal transplant recipients, which included 20 recipients with false-positive class II Luminex DSA, to check whether the DSA detected were really donor specific or not. The results showed that although 20/125 patients had some antibodies detected in their serum, they were not against the donor HLA antigens, as per the HLA typing reports of the donors. When compared to SAB assay, LXM showed more discrepant results, particularly to class II DSA. Conclusion: In conclusion, LXM, if used in combination with SAB assay and HLA typing of donors if necessary for virtual cross match, will help in avoiding unnecessary exclusion of donors for renal transplant recipients and also for post transplant monitoring of recipients, especially in cadaveric donor transplants.

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Section: Discussionmentioning

confidence: 99%

Donor-specific antibody detection by single-antigen bead assay for renal transplantation: A 2-year experience from South India

Neeraja,

Kesireddy,

Kumar

et al. 2023

Indian J Nephrol

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“…For class II specificities, 60 cases were positive with both assays, 9 cases were positive with only one assay (5 only with IC and 4 only with OL) and 28 cases were negative for anti-HLA class II antibodies in both assays. In the 69 cases with at least one positive specificity, 16 (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25) vs. 16 (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23) specificities were detected as positive per case for OL and IC, respectively (p = 0.057).…”

Section: Comparison Of the Two Non-complement Sab Methodsmentioning

confidence: 98%

Complement and Non-Complement Binding Anti-HLA Antibodies Are Differentially Detected with Different Antigen Bead Assays in Renal Transplant Recipients

Ouranos,

Panteli,

Petasis

et al. 2023

JCM

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Two semi-quantitative, Luminex-based, single-antigen bead (SAB) assays are available to detect anti-HLA antibodies and evaluate their reactivity with complement binding. Sera from 97 patients with positive panel reactive antibody tests (>5%) were analyzed with two SAB tests, Immucor (IC) and One-Lambda (OL), for anti-HLA antibody detection and the evaluation of their complement-binding capacity. IC detected 1608/8148 (mean fluorescent intensity (MFI) 4195 (1995–11,272)) and 1136/7275 (MFI 6706 (2647–13,184)) positive anti-HLA class I and II specificities, respectively. Accordingly, OL detected 1942/8148 (MFI 6185 (2855–12,099)) and 1247/7275 (MFI 9498 (3630–17,702)) positive anti-HLA class I and II specificities, respectively. For the IC assay, 428/1608 (MFI 13,900 (9540–17,999)) and 409/1136 (MFI 11,832 (7128–16,531)) positive class I and II specificities bound C3d, respectively. Similarly, OL detected 485/1942 (MFI 15,452 (9369–23,095)) and 298/1247 (MFI18,852 (14,415–24,707)) C1q-binding class I and II specificities. OL was more sensitive in detecting class I and II anti-HLA antibodies than IC was, although there was no significant difference in the number of class II specificities per case. MFI was higher for complement vs. non-complement-binding anti-HLA antibodies in both assays. Both methods were equal in detecting complement-binding anti-HLA class I antibodies, whereas the C3d assay was more sensitive in detecting complement-binding anti-HLA class II antibodies.

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“…Complement fixing ability can be tested for using a modified SAB method (Gonzales et al., 2017). Using such approaches, Loupy et al.…”

Section: Detection and Definition Of Hla Antibodiesmentioning

confidence: 99%

Forming a new perspective: Post‐structural approaches to determination of donor compatibility and post‐transplant assessment of allograft health

Nadat,

Clark

2024

Int J Immunogenetics

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The purpose of this review is to encourage a new perspective on the question of donor–recipient compatibility and post‐transplant assessment of graft health based on functional measures. The premise is that we should be better sighted on what (and how) the immune system responds toward rather than what is merely there. Continuance of the pursuit of further and better definition of antigens and antibodies is not however discouraged but seen as necessary to improved understanding of the structural correlates of functional immunity. There currently exists, in the opinion of the authors, an opportunity for histocompatibility and immunogenetics laboratories to develop and widen their scope of involvement into these new areas of laboratory activity in support and to the benefit of the transplant programmes they serve.

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Comparison Between Total IgG, C1q, and C3d Single Antigen Bead Assays in Detecting Class I Complement-Binding Anti-HLA Antibodies

Cited by 12 publications

References 16 publications

Donor-specific antibody detection by single-antigen bead assay for renal transplantation: A 2-year experience from South India

Donor-specific antibody detection by single-antigen bead assay for renal transplantation: A 2-year experience from South India

Complement and Non-Complement Binding Anti-HLA Antibodies Are Differentially Detected with Different Antigen Bead Assays in Renal Transplant Recipients

Forming a new perspective: Post‐structural approaches to determination of donor compatibility and post‐transplant assessment of allograft health

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