Comparison between the triamcinolone and bevacizumab subconjunctivals and changes in Interleukin-1 mRNA expression in pterygium

Abstract

Objectives Pterygium is a fibrovascular external ocular mass that grows from the conjunctiva into the cornea. The effect of subconjunctival injection of triamcinolone and bevacizumab has been inadequately investigated worldwide. This study aims to analyse the expression of IL-1 after the injection of triamcinolone and bevacizumab subconjunctiva. Methods All patients are randomized into three groups: the triamcinolone, bevacizumab group, and placebo groups, with 5 patients in each in group. All subjects are injected subconjunctivally one week before surgery, and then surgery is performed with the autograft technique. The main outcome measures include changes in the IL-1 mRNA expression between the triamcinolone, bevacizumab, and placebo groups. Results All samples are completed after one month of follow-up. The changes in blood levels of mRNA IL-1 expression are as follows: 4.81 ± 0.52 in the bevacizumab group, 3.40 ± 2.63 in the triamcinolone group, and 1.08 ± 1.48 in the placebo group ( p = 0.04). In the comparison between groups, there is a significant effect between the bevacizumab and placebo groups, 3.73 ± 1.12 ( p = 0.00), with no significant effect in the triamcinolone group, 1.40 ± 1.12 ( p = 0.06). Conclusion The subconjunctival injection of bevacizumab and triamcinolone before surgery is effective in suppressing inflammation in pterygium .

Current trends in the management of corneal neovascularization

“…Though reported in the animal literature, there is limited literature on the effectiveness of combined subconjunctival bevacizumab and triamcinolone injections for corneal NV in human eyes. This combination approach has been used to prevent neovascularization in patients with pterygium and blepharoconjunctivitis [46,47]. Several clinicians have employed this strategy with varying success rates (Figure 4, Supplemental Digital Content, http://links.lww.com/COOP/A65).…”

OXTR Gene mRNA Expression is Correlated to Prosocial Behavior of Children in the Golden Generation Program of Nusa Tenggara Barat

“…Furthermore, the nucleic acid would be combined with the silica or diatom particles contained in the reagent. (12) OXTR mRNA Quantification OXTR mRNA quantification was performed using real time polymerase chain reaction (RT-PCR) methods with CFX Connect System (Bio-Rad, Hercules, CA USA) based on literature. (13) The PCR conditions were following the previous research protocol (14,15), with forward primer sequence: GCAGGGCATCCCAACTCG; and reverse sequence: AAAATGAGCGGGAATCCTCTACC.…”