Cattle rearing is the most important activities practiced by Maasai pastoralists in Kajiado Central Sub-County, Kenya. However, its role has been hampered by occurrence of Brucellosis and other pathogenic conditions of important public health concern. The present study assessed the prevalence and risk factors of brucellosis, and presence of pathogenic bacteria in fermented cow milk obtained from respondents in Kajiado Central Sub-County. The study design was a crosssectional where 114 fermented milk samples were sampled. From each household, one fermented milk sample was obtained and assessed for brucellosis using milk ring test. The milk was transported to the laboratory where presence of bacteria was determined using standard