Comparison between selected hormone and protein levels in serum and prostate tissue homogenates in men with benign prostatic hyperplasia and metabolic disorders

Abstract

PurposeThe purpose of the study was to assess the relationship between changes in the levels of selected hormones in serum and prostate tissue homogenate in regard to metabolic disorders in patients with diagnosed, surgically treated benign prostatic hyperplasia (BPH).Patients and methodsThe study involved a group of 154 men with a diagnosis of BPH with metabolic syndrome (MetS) and without MetS. The serum levels of the hormones – total testosterone, free testosterone, insulin, dehydroepiandrosterone sulfate, estradiol, luteinizing hormone, sex hormone binding globulin (SHBG), and insulin-like growth factor-1 (IGF-1) – were determined using the ELISA method. Prostate tissue sections obtained from the patients during transurethral resection of the prostate were frozen in liquid nitrogen. We determined the levels of the same hormones.ResultsThere was a statistically significant difference between the groups in terms of serum SHBG levels, but not in the prostate tissue SHBG levels. A similar relationship was observed in regard to IGF-1, the serum levels of which were significantly higher in patients with MetS. MetS had an effect on the ratio of hormone levels in serum to their levels in the prostate tissue. Correlations between the levels of biochemical parameters and the levels of hormones in serum and the prostate tissue of BPH patients with and without MetS demonstrate that serum SHBG levels correlated weakly with waist size and triglyceride levels.ConclusionThe occurrence of MetS in BPH patients was associated with changes in the levels of hormones and proteins. These changes, however, were not always equivalent to changes in the levels of these parameters in prostate tissue. It should also be mentioned that MetS in BPH patients had an influence on a quantitative balance between the levels of SHBG in serum and prostate tissue.

The association between metabolic syndrome and lower urinary tract symptoms suggestive of benign prostatic hyperplasia in aging males: evidence based on propensity score matching

“…One prospective study by Yue et al revealed that MetS may accelerate the clinical progression of BPH in middle-aged and older men (13). The conclusions reached by some other studies were also in line with Yue's study (14)(15)(16)(17)(18). However, a study that examined Korean men aged 50 years old proposed a conflicting conclusion (19), and relevant studies performed in Chinese (20), Japanese (21), and American (22) populations echoed this finding.…”

Serum coiled‑coil domain containing 25 protein as a potential screening/diagnostic biomarker for cholangiocarcinoma

“…In the present study, CCDC25 expression in cancerous tissues, as determined by immunohistochemistry, was correlated with serum CCDC25 level, suggesting that CCA cells are the major source of CCDC25 in the sera. Certain studies have reported that protein levels in serum samples may differ from protein expression levels in tissue due to a modification process during protein translocation (28,29). Therefore, to further validate that serum CCDC25 is mainly derived from CCA tissue, the association of serum and tissue CCDC25 expression levels should be verified using a larger number of paired samples.…”

Influence of metabolic syndrome on the relationship between fatty acids and the selected parameters in men with benign prostatic hyperplasia

“…Many authors have examined the effects of testosterone (T), DHT, and estrogen (E) upon BPH and LUTS. Their results, however, are not consistent [ 44 – 46 ].…”