2008
DOI: 10.1128/jcm.01416-07
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Comparison between Quantitative Nucleic Acid Sequence-Based Amplification, Real-Time Reverse Transcriptase PCR, and Real-Time PCR for Quantification ofLeishmaniaParasites

Abstract: DNA or RNA amplification methods for detection of Leishmania parasites have advantages regarding sensitivity and potential quantitative characteristics in comparison with conventional diagnostic methods but are often still not routinely applied. However, the use and application of molecular assays are increasing, but comparative studies on the performance of these different assays are lacking. The aim of this study was to compare three molecular assays for detection and quantification of Leishmania parasites i… Show more

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Cited by 87 publications
(83 citation statements)
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“…The diagnosis can also be confirmed with molecular methods (i.e. PCR), using various clinical specimens (peripheral blood, bone marrow, spleen), with high sensitivity and specificity rates [128,130].…”
Section: Visceral Leishmaniasismentioning
confidence: 99%
“…The diagnosis can also be confirmed with molecular methods (i.e. PCR), using various clinical specimens (peripheral blood, bone marrow, spleen), with high sensitivity and specificity rates [128,130].…”
Section: Visceral Leishmaniasismentioning
confidence: 99%
“…In four cases, Leishmania species identification was performed using polymerase chain reaction (PCR) according to a previously described protocol. 16,17 In accordance with the Brazilian Ministry of Health recommendations for the management of ATL, 18 initial treatment consisted of the administration of pentavalent antimonials (intravenous glucantime 15 mg/kg per day for 20 days for CL and 20 mg/kg per day for 30 days for MCL) or pentamidine (three doses of 4 mg/kg per day administered every other day).…”
Section: Methodsmentioning
confidence: 99%
“…All samples were analyzed with quantitative reverse-transcriptase PCR (qRT-PCR) 14 ( Table 1 ). This method is able to amplify all Leishmania species and quantifies parasitemia in patient samples.…”
Section: Quantitative Reverse-transcriptase Pcr (Qrt-pcr)mentioning
confidence: 99%