Comparison Between Phenotypic Confirmatory Test &amp; Double Disc Synergy Test in Detection of Extended Spectrum b-Lactamases Producers Among Gram-Negative Bacilli

Chowdhury, Abu Hena Md Saiful Karim; Nandi, Sukumar; Rahman, Mahbubur; Karim, Asm Ashanul; Mamtaz, Syeda Shanoor Hasina; Ara, Nura Nasrin Rowshan; Sultana, Sabrina

doi:10.3329/cmoshmcj.v15i2.31795

Cited by 4 publications

(4 citation statements)

References 6 publications

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“…Sinerjistik etkiyi saptayan testler çift disk sinerji testi adı ile genişlemiş spektrumlu beta laktamaz (GSBL) saptanmasında, antagonist etkiyi saptayan testler D zon testi adı ile eritromisin ve klindamisin arasındaki antagonistik etkiyi saptamada yaygın şekilde kullanılmıştır (5,28) . GSBL oluşturan bakterileri saptamak için kullanıldığında çift disk sinerji testinin çeşitli çalışmalarda sensitivitesi %83-95, spesifitesi %76-100, pozitif prediktif değeri %84-100 ve negatif prediktif değeri %78-92 olarak bulunmuştur (4,7) . Bu çalışmada maddi ve zamansal kaygılar güdüldüğü için çift disk sinerji yöntemi tercih edilmiştir.…”

Section: Discussionunclassified

Investigation of In Vitro Synergistic Effect of Niclosamide with Mupirocin in Staphylococcus aureus Strains by Screening Test

CİHAN

Kaygusuz

2022

ANKEM Dergisi

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Staphylococcus aureus taşıyıcılarında kullanılan antibiyotiklere direncin artması alternatif tedavi arayışını gündeme getirmiştir. Bu araştırma ile S. aureus izolatlarında niklozamidin mupirosin ile in vitro sinerjistik etkisinin olup olmadığının incelenmesi hedeflenmiştir. 2021 yılında laboratuvarımıza gönderilen örneklerden 25 metisiline duyarlı S. aureus (MSSA) ve 25 metisiline dirençli S. aureus (MRSA) izolatı toplanmıştır. İzolatlarda çift disk sinerji yöntemi uygulanarak niklozamid ile mupirosin arasında in vitro sinerjistik etki olup olmadığı incelenmiştir. Çalışmada test edilen izolatlarda iki antimikrobiyal ilaç arasında herhangi bir sinerji saptanmamıştır. İzolatların sadece MRSA grubundaki iki tanesinde mupirosine direnç gözlenmiş olup, diğer izolatların tümünün mupirosine duyarlı olduğu bulunmuştur. S. aureus izolatlarında niklozamidin mupirosin ile in vitro sinerjistik etkisinin olmadığı gözlenmiştir.

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Section: Discussionunclassified

Investigation of In Vitro Synergistic Effect of Niclosamide with Mupirocin in Staphylococcus aureus Strains by Screening Test

CİHAN

Kaygusuz

2022

ANKEM Dergisi

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“…Enhanced zones of inhibition of ceftriaxone and/or ceftazidime discs towards amoxicillinclavulanic acid disc confirms ESBLs production (Figure 3) [44]. The sensitivity of DDS method is reported between 83.1% and 92.9% [45,46] whereas its specificity is reported at 100% [45,46]. The limitation of DDST is failure to detect ESBL phenotypes among co-producers of ESBLs and Amp-C β-lactamases [44].…”

Section: Double Disc Synergy Test (Ddst)mentioning

confidence: 99%

Beta-lactam antibiotics and extended spectrum beta-lactamases

Silago¹

2021

GSC Adv. Res. Rev.

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Extended spectrum beta-lactamases (ESBLs) are enzymes produced by bacteria, mostly members of the family Enterobacteriaceae commonly Escherichia coli and Klebsiella pneumoniae. ESBLs hydrolyze the beta-lactam ring of beta-lactam antibiotics making these antibiotics ineffective therefore rendering the bacteria resistance against beta-lactam antibiotics. The global upsurge of ESBLs producing bacteria causing both hospital and community acquired infections mostly urinary tract infections, pneumonia and bloodstream infections, threatens the effectiveness of infectious diseases treatment. ESBL families; TEM, SHV and CTX-M are globally disseminated and frequently detected in clinical isolates as well as colonization and contamination isolates. Various laboratory detection methods of ESBLs producing Gram negative bacteria are available. These methods; phenotypic methods, automated methods and molecular-based methods are varying in sensitivity and specificity, need of technical expertise, and rapidness. Therefore, they should be clearly understood before being employed for routine or research use for detection of ESBLs production among Enterobacteriaceae. In addition, understanding the mode of action and mechanisms of resistance to beta-lactam antibiotics, and the epidemiology of ESBLs producing bacteria is of paramount.

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“…5 ESBLs producing bacteria are crucial threat for patients in the hospital, long-term care facilities and the community for their highly disseminating characteristics. 6 ESBL producing E. coli strains also show resistance to other classes of antibiotic making the pathogen hard to treat. [7][8] Extended-Spectrum b -Lactamase (ESBL), comes under Ambler class A and D b-lactamase enzymes, 9 They mediate resistance to extended spectrum (third and fourth generation) cephalosporins (e.g.…”

Section: Introductionmentioning

confidence: 99%

A Guide Towards the Phenotypic Detection of Extended-spectrum β-lactamases Production in Enterobacteriaceae: Alone or in Presence of Other Interfering Enzymes

Das,

Mahapatra,

Mazumder

2023

J. Pure Appl. Microbiol.

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Antimicrobial Resistance (AMR) has been regarded as a major public health concern as a reason of millions of deaths. Extended-spectrum β-lactamase (ESBL) is considered as a leading factor contributing to this and limiting its treatment. Thus, ESBL producing Enterobacteriaceae should be discriminated from those having other mechanism conferring resistance. Several phenotypic methods have been evaluated for this purpose. Some of these are based on conventional method (DDST, CDT, ESBL E-test, Cica-β test) while others depend on automated systems (VITEK 1, VITEK 2, Phoenix, MicroScan). All the conventional methods have been found to be more specific, sensitive and cost effective than any of the automated system though they are easy to perform and interpret. Automated system also fails to detect ESBL in presence of other interfering enzymes such as AmpC, MBL or K1 enzyme. ESBL can be detected by using third-generation cephalosporin (cefotaxime or ceftazidime) or monobactam (aztreonam) in combination with clavulanate. AmpC can be distinguished by using cloxacillin-containing agar, fourth-generation cephalosporin (cefepime) or phenylboronic acid. MBL producers remain unaffected in presence of clavulanate but gets inhibited by carbapenems (imipenem, meropenem) in combination with EDTA. Cefpodoxime-clavulanate and ceftazidime- clavulanate combinations are reliable for K1 enzyme detection but are not suitable for distinguishing blaCTX–M1.

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Comparison Between Phenotypic Confirmatory Test & Double Disc Synergy Test in Detection of Extended Spectrum b-Lactamases Producers Among Gram-Negative Bacilli

Cited by 4 publications

References 6 publications

Investigation of In Vitro Synergistic Effect of Niclosamide with Mupirocin in Staphylococcus aureus Strains by Screening Test

Investigation of In Vitro Synergistic Effect of Niclosamide with Mupirocin in Staphylococcus aureus Strains by Screening Test

Beta-lactam antibiotics and extended spectrum beta-lactamases

A Guide Towards the Phenotypic Detection of Extended-spectrum β-lactamases Production in Enterobacteriaceae: Alone or in Presence of Other Interfering Enzymes

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