Comparison between Hypericum triquetrifolium Leaves and Derived Calli in Essential Oil Content

Abstract

The analysis of essential oil using the in-tube extraction technique (ITEX) from the plant leaves and derived calli (fresh calli, dry calli and cell suspension culture) of Hypericum triquetrifolium Turra., initiated from leaves. The plant grows wild in Kurdistan region of Iraq. Studied parameters were determined using in-tube extraction coupled with gas chromatography -mass spectrometry system (ITEX/ GC-MS). A total of 33 compounds were identified as essential oils in leaves, the dominant constituents were measured such as Hexenal, (E) (12.63%), Octane, 2,3,3-trimethyl (11.36%), Pentadecane, 7-methyl-(9.7%), Undecane (6.15%) and alpha. -Pinene (5.75%), while the analysis of fresh calli derived from leaves showed 22 types of essential oil; Dodecane (23.78%), Nonane, 3-methyl-(10.45%), Limonene (9.68%), Furan, 2-pentyl-(9.11%), Toluene (8.18%) and Undecane (7.45%). On the other hand, 21 oil components were found in dry calli; the major compounds were identified as Limonene (17.18%), Undecane (12.21%), Beta.-Myrcene (5.51%) and Toluene (4.93%). However, only 23 oil components were determined in cell suspension culture, the main essential oils were; Undecan (42.92%), Octane, 2,4,6-trimethyl (13.71%), Oxirane, 2-(1,1-dimethylethyl)-3-methyl (9.84%), Limonene (6.69%) and Toluene (2.98%).

Lemongrass Essential Oil Shows an Antiproliferative Effect on Lung Cancer Cells and Its Role in Expression of P53 Gene

“…The flow rate of 1.35 ml/min and the carrier gas was helium, carrying the contents of the sample from the injector straight the column to the detector. The mass spectrometry (MS) mode was electron impact (EI) (9).…”

GC-Mass Spectormetry Profiling of Essential Oils from Aerial Parts of Hypericum triquetrifolium Turra using ITEX and HD Methods

“…It usually grows in the lower forest zone; in the moist steppe zone, and it is found on rocky mountain slopes, stony hillsides, and stepping plains, sometimes near streams, in vineyards, gardens, and fields, on sandstone, clay, loam, gravel [3] . The traditional medical uses of H. triquetrifolium are consequences of their contents with various bioactive molecules, including; naphthodianthrones, hypericin and pseudohypericin [4] , hyperforin and adhyperforin [5] , essential oils [6] , xanthones [7] , phenolic compounds (tannic acid, p-OHbenzoic acid, chlorogenic acid, and caffeic acid), procyanidins, and other water-soluble compounds [8] , which have various biological values; namely antioxidant, anti-inflammatory, antidepressant, antimicrobial, antifungal, antiviral, and anticonvulsant activities.…”