Comparison between cerebrospinal fluid and serum levels of myelin-associated glycoprotein, total antioxidant capacity, and 8-hydroxy-2′-deoxyguanosine in patients with multiple sclerosis

Khajenobar, Negin Bodaghi; Mahboob, Soltanali; Nourazarian, Alireza; Shademan, Behrouz; Laghousi, Delara; Moayed, Zohre Bagheri; Hassanpour, Mehdi; Nikanfar, Masoud

doi:10.1016/j.clineuro.2020.106377

Cited by 10 publications

(14 citation statements)

References 25 publications

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“…Given the clinical and neurobiological complexity of the etiology and pathogenesis of MS, identifying biomarkers for the diagnosis of MS is challenging. The precise mechanisms of tissue and neuronal injury in this disease, including the relationships among inflammation, activated macrophages, axonal injury, and demyelination, have not been fully elucidated 13 . It has been shown that MS patients on drug therapy have reduced serum and CSF levels of leptin.…”

Section: Discussionmentioning

confidence: 99%

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Investigation of serum levels of orexin‐A, transforming growth factor β, and leptin in patients with multiple sclerosis

Moharami

Nourazarian

Nikanfar

et al. 2021

Clinical Laboratory Analysis

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Background Multiple sclerosis (MS) is a chronic inflammatory and autoimmune disease affecting various inflammatory and nutritional parameters. Therefore, this study aimed to investigate the relationship between the Body Mass Index (BMI) of MS patients and the serum levels of leptin, orexin‐A, and Transforming Growth Factor β (TGF‐β). Methods This cross‐sectional study included 25 patients suffering from MS and 40 healthy individuals as the case and control groups, respectively. The serum levels of leptin, orexin‐A, and TGF‐β were assessed in the participants using the Enzyme‐Linked Immunosorbent Assay methods. Moreover, data were analyzed using the descriptive statistical indices, t‐test, chi‐square test, and linear regression test. Results According to our results, the participants’ mean age was 38.04 ± 7.53 and 40.23 ± 5.88 in the case and control groups, respectively. Also, the groups were not significantly different in gender, age, alcohol consumption, and smoking (p > 0.05). It was found that the mean serum levels of orexin‐A and TGF‐β were significantly lower in the MS patients compared to the control group, while the mean serum leptin levels were significantly higher (42.8 vs. 18.9 ng/ml, p < 0.001). Moreover, there was no significant relationship between the BMI of the MS patients and their serum levels of orexin‐A, TGF‐β, and leptin (p > 0.05). Conclusions In conclusion, we found significantly lower levels of orexin‐A and TGF‐β and a significantly higher level of leptin in the MS patients compared to the control group. In addition, there was no significant relationship between the BMI and the serum levels of orexin‐A, TGF‐β, and leptin in MS patients.

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Section: Discussionmentioning

confidence: 99%

“…It is also an effective tool for assessing health status and can be used in studying the prevalence of obesity. This index indicates how well a particular person's height and weight match 13 . As mentioned previously, leptin, TGF‐β, and orexins may play a role in the pathophysiology of MS.…”

Section: Introductionmentioning

confidence: 99%

Investigation of serum levels of orexin‐A, transforming growth factor β, and leptin in patients with multiple sclerosis

Moharami

Nourazarian

Nikanfar

et al. 2021

Clinical Laboratory Analysis

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“…Multiple sclerosis (MS) is a neurodegenerative disorder that causes in ammation in the central nerve system (CNS), which leads to neuropathy [19,20]. According to epidemiological studies, its prevalence is closely associated with nutrition, and it commonly occurs in people with high levels of fatty acid and Vitamin D de ciency [21].…”

Section: Discussionmentioning

confidence: 99%

The Relationship Between Serum Orexin A, TGF-β, and Leptin Levels with Body Mass Index in Multiple Sclerosis Patients

Moharami

Nourazarian

Nikanfar

et al. 2021

Preprint

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Backgrounds: Multiple Sclerosis (MS) is a chronic inflammatory and autoimmune disease linked to several inflammatory and dietary parameters. This study was carried out to determine the relationship between serum leptin, orexin-A, and TGF-β levels with BMI in MS patients.Methods and results: In this cross-sectional study, 25 relapsing-remitting multiple sclerosis (RRMS) patients and 40 healthy controls were enrolled. The serum level of Leptin, Orexin-A, and TGF- were measured by the Enzyme-linked immunosorbent assay (ELISA). The data was analyzed using descriptive statistics, t-test, Chi-square test, and Linear regression test. A total of 65 volunteers, including 25 MS patients and 40 healthy, were enrolled in the study. The mean age of individuals in the case and control groups was 38.04 ± 7.53 and 40.23 ± 5.88. There were no statistically significant differences between the case and control groups regarding gender, age, alcohol, and cigarette use (P>0.05). The mean serum levels of Orexin-A and TGF-ß were lower among multiple sclerosis patients than in healthy controls, but leptin was higher (42.8 vs. 18.9 ng/ml, P<0.001). The relationship between BMI and serum levels of Orexin-A, TGF-ß, and Leptin among Multiple Sclerosis patients was not statistically significant (P > 0.05).Conclusion: Our results showed that the serum levels of Orexin-A and TGF-β were significantly lower. The serum level of leptin was higher among multiple sclerosis patients than among healthy controls. Also, there was no statistically significant relationship between BMI and serum levels of Orexin-A, TGF-ß, and Leptin among multiple sclerosis patients.

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“…MS patients were diagnosed by an experienced neurologist using standard serum and CSF biomarkers. The McDonald criteria were used to interpret the MRI ndings [27]. The study focused on individuals with relapsing-remitting multiple sclerosis (RRMS).…”

Section: Sample Selectionmentioning

confidence: 99%

“…According to established CSF collection protocols, immediately after lumbar puncture, to extract cell-free CSF, CSF was centrifuged at 400 g for 15 min. CSF and RNA were extracted from cell-free cultures and stored at 80°C until all RNA was extracted [27].…”

Section: Samplingmentioning

confidence: 99%

To Study the Relationship between the Expression of MiR-21, MiR-155, Mir-182, and Mir-437 and Inflammatory Factors in the Cerebrospinal Fluid of Patients with Multiple Sclerosis

Karamad¹,

Söğütlü²,

Abbasi³

et al. 2021

Preprint

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Background: MicroRNAs (miRNAs) affect the differentiation and function of inflammatory cells. In addition, miRNAs can regulate the expression of pro-inflammatory genes in neuron cells. Therefore, the relationship between the expression of miRNAs and inflammatory markers in the cerebrospinal fluid of individuals with multiple sclerosis was investigated.Methods: RT-PCR determined the expression levels of MiR-21, MiR-155, Mir-182, and Mir-437 in CSF samples from patients with multiple sclerosis (MS) and the control group. The levels of the inflammatory cytokines IL -1β, IL -6, and TNF-α in CSF were measured by ELISA. The level of high-sensitivity C-reactive protein (hs-CRP) was measured by the quantitative turbidometric technique.Results: The expression of miRNAs and inflammatory factors were higher in the CSF of patients with MS than in the control group, and this difference was significant (P <0.05). The results of Receiver Operating Characteristics (ROC) analysis show that the area under the curve was obtained for miRNA-21 (AUC = 0.97, P <0.0001), miRNA-182 (AUC = 0.97, P <0.0001), and miRNA-155 (AUC = 0.96, P <0.0001). The highest rate of correct diagnoses of MS was associated with miRNA-155 in CSF. The relationship between inflammatory cytokines and miRNA-21, miRNA-155, and miRNA-182 was statistically significant, but there was an indirect and moderate correlation between miRNA-437 and hs-CRP, which were also statistically significant.Conclusion: Our results showed that the CSF levels of IL -1β, IL -6, TNF-α, and hs-CRP, and selected miRNAs, serve as biomarkers of central nervous system (CNS) inflammation and neurodegenerative processes in patients with MS.

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Comparison between cerebrospinal fluid and serum levels of myelin-associated glycoprotein, total antioxidant capacity, and 8-hydroxy-2′-deoxyguanosine in patients with multiple sclerosis

Cited by 10 publications

References 25 publications

Investigation of serum levels of orexin‐A, transforming growth factor β, and leptin in patients with multiple sclerosis

Investigation of serum levels of orexin‐A, transforming growth factor β, and leptin in patients with multiple sclerosis

The Relationship Between Serum Orexin A, TGF-β, and Leptin Levels with Body Mass Index in Multiple Sclerosis Patients

To Study the Relationship between the Expression of MiR-21, MiR-155, Mir-182, and Mir-437 and Inflammatory Factors in the Cerebrospinal Fluid of Patients with Multiple Sclerosis

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