Comparison Between Capillary Electrophoresis and High Performance Liquid Chromatography for Detection and Quantification of Hb Constant Spring [Hb CS; α142, Term→Gln (TAA>CAA IN α2)]

Abstract

Hb Constant Spring [Hb CS; α142, Term→Gln (TAA>CAA in α2)] is often missed by routine laboratory testing since its mRNA as well as gene product are unstable and presented at a low level in peripheral blood. This study aimed to analyze the efficacy of capillary electrophoresis (CE) and high performance liquid chromatography (HPLC) for detecting and quantifying Hb CS in 19 heterozygotes and 14 homozygotes with Hb CS as well as 10 Hb H-CS disease subjects who were detected by molecular analysis. In the CE electrophoregram, Hb CS was seen at zone 2 and was observed in all samples, while the chromatogram of Hb CS peaks was found in 26.32% heterozygotes, 42.86% homozygotes and 90% Hb H-CS disease subjects, respectively. In addition, the Hb CS levels in each group of subjects quantified by CE were significantly higher than those quantified by HPLC. Based on the CE method, the lowest Hb CS level was found in the heterozygotes, whereas the highest level was found in the Hb H-CS disease patients. Therefore, the CE method was superior to the HPLC method for detecting Hb CS. Furthermore, the level of Hb CS quantified by CE proved useful in screening heterozygotes and homozygotes with Hb CS as well as Hb H-CS disease.

Hemoglobin Constant Spring (Hb CS) Missed by HPLC in an Hb E Trait Pregnancy Resulting in Hb H-CS Disease in a Thai Girl: Utility of Capillary Electrophoresis

“…In contrary with the current study, Hb CS heterozygotes carrying Hb E, another unstable hemoglobin variant of the b-globin chain which is frequently found in the Thai population, can be detected by CE but not by HPLC. Consistent with the previous study, the CE had higher sensitivity than HPLC for detection of Hb CS [16]. Thus, CE analysis is necessary in a couple which is discordant for thalassemia that is one partner has a-thalassemia-1 and the other has b-thalassemia or Hb E trait.…”

First recorded case of haemoglobin SC in sudan

“…CE was performed in completely automatic equipment (Cebia-Model 2007). Control samples were prepared using a mixture of Hbs A, F, S and C and included in each experiment alongside the test samples [18].…”

“…Samples on Guthrie cards were stored at -20 o C with dessicant material to keep specimens dry. CE-HPLC was used as a confirmatory test after Capillary electrophoresis [18]. Guthrie card samples were analyzed, using CE-HPLC [19].…”

Detection of Co-inheritance of Hb Hope and Hb Constant Spring in Three Thai Samples by Capillary Electrophoresis

“…Whether the CE, which has proven superior to the HPLC in the detection of Hb constant spring [Hb CS; a142, Term ? Gln (TAA [ CAA IN a2)] trait [7], could diagnose the co-inheritance of Hb Hope and Hb CS is unclear. In this study, we reported the CE electrophoregram of the samples with co-inheritance of Hb Hope and Hb CS.…”