Comparison between a modified counterimmunoelectrophoresis test and the indirect fluorescent-antibody test for detection of antibodies to rabies virus in human sera

Diaz, Ana María; Myers, Donald M.

doi:10.1128/jcm.14.4.446-448.1981

Cited by 10 publications

(1 citation statement)

References 6 publications

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“…The IFA is reported to be more sensitive than the neutralization test (NT) in detecting antibody early in the immune response, making it valuable for diagnosis. [ 39 40 ] The IFA can also be used in seroepidemiological surveys. It is a rapid test and is economical and easy to perform.…”

Section: Laboratory Investigationsmentioning

confidence: 99%

Antemortem diagnosis and prevention of human rabies

Madhusudana

Sukumaran

2008

Ann Indian Acad Neurol

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Human rabies still continues to be a significant health problem in India and other developing countries where dogs are the major vectors of transmission. Rabies in humans can present in two clinical forms, i.e., furious and paralytic. While diagnosis of furious rabies can be made based on the typical symptoms and signs, paralytic rabies poses a diagnostic dilemma to the neurologists who may encounter these cases in their practice. Although there are certain clinical features that distinguish this disease from other forms of Guillain-Barre syndromes, confirmation of diagnosis may require laboratory assistance. Conventional techniques such as antigen detection, antibody assays and virus isolation have limited success. The recently introduced molecular techniques show more promise in confirming the cases of paralytic rabies. There has not been much success in the treatment of confirmed rabies cases and recovery from rabies is extremely rare. Therefore, preventive measures of this dreaded disease after an exposure become extremely important. The present article reviews the current status of human rabies with regard to antemortem diagnosis, disease management and post-exposure prophylaxis.

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Section: Laboratory Investigationsmentioning

confidence: 99%

Antemortem diagnosis and prevention of human rabies

Madhusudana

Sukumaran

2008

Ann Indian Acad Neurol

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Comparative Analysis of Routine Laboratory Diagnostic Tests for Rabies

Kashte

Sherikar

Pingale³

2011

Indian J. Virol.

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Present study was undertaken to compare various routine laboratory diagnostic tests for rabies detection. Seller's staining, mouse inoculation test (MIT), Dot-ELISA, Agar gel precipitation test (AGPT) and counter immunoelectrophoresis test (CIET) were the main basic tests performed in the laboratory for the rabies diagnosis. Out of 200 brain specimens, Negri bodies were observed in 52 brain samples by Seller's staining. Rabies virus was isolated in 56 samples by intra-cerebral inoculation in newborn Swiss-albino mice. Dot-ELISA and AGPT could detect rabies antigen in 55 and 57 samples respectively. Comparative analysis revealed that the CIET is the most sensitive and rapid test among performed diagnostic tests.

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