Comparison and combination of rotational imaging optical coherence tomography and selective plane illumination microscopy for embryonic study

Abstract

Several optical imaging techniques have been applied for high-resolution embryonic imaging using different contrast mechanisms, each with their own benefits and limitations. In this study, we imaged the same E9.5 mouse embryo with rotational imaging optical coherence tomography (RI-OCT) and selective plane illumination microscopy (SPIM). RI-OCT overcomes optical penetration limits of traditional OCT imaging that prohibit full-body imaging of mouse embryos at later stages by imaging the samples from multiple angles. SPIM enables high-resolution, 3D imaging with less phototoxicity and photobleaching than laser scanning confocal microscopy (LSCM) by illuminating the sample with a focused sheet of light. Side by side comparisons are supplemented with co-registered images. The results demonstrate that SPIM and RI-OCT are highly complementary and could provide more comprehensive tissue characterization for mouse embryonic research. Feneley, and R. P. Harvey, "Cardiac septal and valvular dysmorphogenesis in mice heterozygous for mutations in the homeobox gene Nkx2-5," Circ. Res.

Multimodal Optical Medical Imaging Concepts Based on Optical Coherence Tomography

“…The study included corneal SHG imaging of diseases and healthy state as well as collagen microstructural response to changes in intraocular pressure correlating the results also with OCT. Multimodal microscopy demonstrated improved molecular and multi-scale structural contrast also for ex-vivo and in-vivo skin imaging [174,175]. A recent study demonstrated the combination of tomographic OCT with selective plane illumination microscopy (SPIM) for the study of mouse embryon development employing fluorescence microscopy with the potential to be also combined with MPM [176]. MPM has been shown to provide important metabolic information in addition to cellular structural details in various skin disorders and diseases such as basal cell carcinoma and other skin cancers, hemangioma, psoriasis, pigmented lesions, or connective tissue diseases [177,178].…”

Embryonic Mouse Cardiodynamic OCT Imaging

“…With the further improvement in the imaging speed of Brillouin microscopy [ 105 ] as well as the integration of the two systems [ 106 ], OCT-guided direct biomechanical assessment in the beating mouse embryonic heart is potentially possible. In another multimodality configuration, the lack of molecular contrast from OCT was complemented by light-sheet microscopy as well as the establishment of rotation-based OCT imaging [ 107 , 108 ]. In addition to imaging, a number of manipulation methods have been established for mouse embryos, such as controlled microinjection to the blood circulation [ 109 , 110 ] and cardiac optogenetic pacing [ 111 ], which set a platform for mechanistic studies investigating the interplay between molecular and biomechanical factors in mammalian cardiogenesis.…”

Biophotonics feature: introduction

“…In this feature issue of Biomedical Optics Express, we have collected several papers [3][4][5] that represent some of the technologies discussed at the NTM symposium that moved the field, both in terms of the technology and also the biological applications.…”