Comparing transcriptome expression profiles to reveal the mechanisms of salt tolerance and exogenous glycine betaine mitigation in maize seedlings

Chen, Fenqi; Peng, Fang; Zeng, Wenjing; Ding, Yulong; Zhuang, Zhixiong; Peng, Yunling

doi:10.1371/journal.pone.0233616

Cited by 30 publications

(20 citation statements)

References 73 publications

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“…The reaction volume included 10 μL SuperReal PreMix Plus, 6 μL ddH 2 O, 0.8 μL forward primer (10 μmol/L), 0.8 μL reverse primer, 0.4 μL Rox reference dye and 2 μL template cDNA. The amplification procedure was described with reference to Chen et al (2020) . Actin gene was used as the internal reference gene ( Zeng et al, 2019 ), and the gene expression was analyzed by 2 –ΔΔCT calculation method.…”

Section: Methodsmentioning

confidence: 99%

“…Maize ( Zea Mays L.) is the most widely grown and important food, feed, and industrial feedstock crop in the world ( Niu et al, 2019a ). A disadvantage of maize is that it is sensitive to a variety of abiotic stresses (such as salt stress, drought stress, and low temperature stress) during its seedling life ( Chen et al, 2020 ). In China, maize planting areas are mostly distributed in arid and semi-arid areas, accounting for 2/3 of cultivated land ( Du et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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Network Analysis of Different Exogenous Hormones on the Regulation of Deep Sowing Tolerance in Maize Seedlings

Chen

Bai

et al. 2021

Front. Plant Sci.

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The planting method of deep sowing can make the seeds make full use of water in deep soil, which is considered to be an effective way to respond to drought stress. However, deep sowing will affect the growth and development of maize (Zea mays L.) at seedling stage. To better understand the response of maize to deep sowing stress and the mechanism of exogenous hormones [Gibberellin (GA3), Brassinolide (BR), Strigolactone (SL)] alleviates the damaging effects of deep-sowing stress, the physiological and transcriptome expression profiles of seedlings of deep sowing sensitive inbred line Zi330 and the deep-tolerant inbred line Qi319 were compared under deep sowing stress and the conditions of exogenous hormones alleviates stress. The results showed that mesocotyl elongated significantly after both deep sowing stress and application of exogenous hormones, and its elongation was mainly through elongation and expansion of cell volume. Hormone assays revealed no significant changes in zeatin (ZT) content of the mesocotyl after deep sowing and exogenous hormone application. The endogenous GA3 and auxin (IAA) contents in the mesocotyl of the two inbred lines increased significantly after the addition of exogenous GA3, BR, and SL under deep sowing stress compared to deep sowing stress, while BR and SL decreased significantly. Transcriptome analysis showed that the deep seeding stress was alleviated by GA3, BR, and SLs, the differentially expressed genes (DEGs) mainly included cellulose synthase, expansin and glucanase, oxidase, lignin biosynthesis genes and so on. We also found that protein phosphatase 2C and GA receptor GID1 enhanced the ability of resist deep seeding stress in maize by participating in the abscisic acid (ABA) and the GA signaling pathway, respectively. In addition, we identified two gene modules that were significantly related to mesocotyl elongation, and identified some hub genes that were significantly related to mesocotyl elongation by WGCNA analysis. These genes were mainly involved in transcription regulation, hydrolase activity, protein binding and plasma membrane. Our results from this study may provide theoretical basis for determining the maize deep seeding tolerance and the mechanism by which exogenous hormones regulates deep seeding tolerance.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Network Analysis of Different Exogenous Hormones on the Regulation of Deep Sowing Tolerance in Maize Seedlings

Chen

Bai

et al. 2021

Front. Plant Sci.

Self Cite

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“…Many experiments also proved the benefit of the external application of betaine on the plant under salt stress. Experiments by Chen et al showed that exogenous glycine betaine mitigated salt stress in maize seedlings [57]. In addition, betaine is involved in many metabolic processes.…”

Section: Organic Substancesmentioning

confidence: 99%

A Review on Plant Responses to Salt Stress and Their Mechanisms of Salt Resistance

et al. 2021

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Nowadays, crop insufficiency resulting from soil salinization is threatening the world. On the basis that soil salinization has become a worldwide problem, studying the mechanisms of plant salt tolerance is of great theoretical and practical significance to improve crop yield, to cultivate new salt-tolerant varieties, and to make full use of saline land. Based on previous studies, this paper reviews the damage of salt stress to plants, including suppression of photosynthesis, disturbance of ion homeostasis, and membrane peroxidation. We have also summarized the physiological mechanisms of salt tolerance, including reactive oxygen species (ROS) scavenging and osmotic adjustment. Four main stress-related signaling pathways, salt overly sensitive (SOS) pathway, calcium-dependent protein kinase (CDPK) pathway, mitogen-activated protein kinase (MAPKs) pathway, and abscisic acid (ABA) pathway, are included. We have also enumerated some salt stress-responsive genes that correspond to physiological mechanisms. In the end, we have outlined the present approaches and techniques to improve salt tolerance of plants. All in all, we reviewed those aspects above, in the hope of providing valuable background knowledge for the future cultivation of agricultural and forestry plants.

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“…Comparing the difference in transcriptional levels between tolerant and sensitive genotypes under stress conditions, the genes related to stress tolerance can be isolated. A transcriptomics study also revealed different upand downregulated transcriptional factors such as MYB, MYBrelated, AP2-EREBP, NAC, and WRKY (Chen F. et al, 2020). This transcriptional profiling study gives a better insight into the understanding of the key components in the plant salt tolerance network which is important for developing more salt-tolerant plants.…”

Section: Transcriptomicsmentioning

confidence: 77%

Crucial Cell Signaling Compounds Crosstalk and Integrative Multi-Omics Techniques for Salinity Stress Tolerance in Plants

et al. 2021

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In the era of rapid climate change, abiotic stresses are the primary cause for yield gap in major agricultural crops. Among them, salinity is considered a calamitous stress due to its global distribution and consequences. Salinity affects plant processes and growth by imposing osmotic stress and destroys ionic and redox signaling. It also affects phytohormone homeostasis, which leads to oxidative stress and eventually imbalances metabolic activity. In this situation, signaling compound crosstalk such as gasotransmitters [nitric oxide (NO), hydrogen sulfide (H2S), hydrogen peroxide (H2O2), calcium (Ca), reactive oxygen species (ROS)] and plant growth regulators (auxin, ethylene, abscisic acid, and salicylic acid) have a decisive role in regulating plant stress signaling and administer unfavorable circumstances including salinity stress. Moreover, recent significant progress in omics techniques (transcriptomics, genomics, proteomics, and metabolomics) have helped to reinforce the deep understanding of molecular insight in multiple stress tolerance. Currently, there is very little information on gasotransmitters and plant growth regulator crosstalk and inadequacy of information regarding the integration of multi-omics technology during salinity stress. Therefore, there is an urgent need to understand the crucial cell signaling crosstalk mechanisms and integrative multi-omics techniques to provide a more direct approach for salinity stress tolerance. To address the above-mentioned words, this review covers the common mechanisms of signaling compounds and role of different signaling crosstalk under salinity stress tolerance. Thereafter, we mention the integration of different omics technology and compile recent information with respect to salinity stress tolerance.

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Comparing transcriptome expression profiles to reveal the mechanisms of salt tolerance and exogenous glycine betaine mitigation in maize seedlings

Cited by 30 publications

References 73 publications

Network Analysis of Different Exogenous Hormones on the Regulation of Deep Sowing Tolerance in Maize Seedlings

Network Analysis of Different Exogenous Hormones on the Regulation of Deep Sowing Tolerance in Maize Seedlings

A Review on Plant Responses to Salt Stress and Their Mechanisms of Salt Resistance

Crucial Cell Signaling Compounds Crosstalk and Integrative Multi-Omics Techniques for Salinity Stress Tolerance in Plants

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