Comparing the endothelialisation of extracellular matrix bioscaffolds with coated synthetic vascular graft materials

Coakley, Daniel; Shaikh, Faisal; O’Sullivan, Kathleen; Kavanagh, Eamon G.; Grace, Pierce A.; Walsh, Stewart R.; McGloughlin, Timothy M.

doi:10.1016/j.ijsu.2015.11.008

Cited by 12 publications

(11 citation statements)

References 46 publications

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“…This endothelial cell motility is a critical aspect of angiogenesis [64]. Finally, we found out a comparable average cell proliferation to previous studies for EC culture over biodegradable scaffolds [63,65]. Despite our progress, a deeper understanding of cell attachment under hemodynamic conditions could provide further clues of changes in cell orientation and morphology.…”

Section: Discussionsupporting

confidence: 69%

Design and Characterization of a Fluidic Device for the Evaluation of SIS-Based Vascular Grafts

et al. 2020

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Currently available small diameter vascular conduits present several long-term limitations, which has prevented their full clinical implementation. Commercially available vascular grafts show no regenerative capabilities and eventually require surgical replacement; therefore, it is of great interest to develop alternative regenerative vascular grafts (RVG). Decellularized Small Intestinal Submucosa (SIS) is an attractive material for RVG, however, the evaluation of the performance of these grafts is challenging due to the absence of devices that mimic the conditions found in vivo. Thereby, the objective of this study is to design, manufacture and validate in silico and in vitro, a novel fluidic system for the evaluation of human umbilical vein endothelial cells (HUVECs) proliferation on SIS-based RVG under dynamical conditions. Our perfusion and rotational fluidic system was designed in Autodesk Inventor 2018. In silico Computational Fluid Dynamics (CFD) validation of the system was carried out using Ansys Fluent software from ANSYS, Inc for dynamical conditions of a pulsatile pressure function measured experimentally over a rigid wall model. Mechanical and biological parameters such as flow regime, pressure gradient, wall shear stress (WSS), sterility and indirect cell viability (MTT assay) were also evaluated. Cell adhesion was confirmed by SEM imaging. The fluid flow regime within the system remains laminar. The system maintained sterility and showed low cytotoxicity levels. HUVECs were successfully cultured on SIS-based RVG under both perfusion and rotation conditions. In silico analysis agreed well with our experimental and theoretical results, and with recent in vitro and in vivo reports for WSS. The system presented is a tool for evaluating RVG and represents an alternative to develop new methods and protocols for a more comprehensive study of regenerative cardiovascular devices.

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Section: Discussionsupporting

confidence: 69%

Design and Characterization of a Fluidic Device for the Evaluation of SIS-Based Vascular Grafts

et al. 2020

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“…Moreover, in vitro experiments with grafts made of synthetic materials currently in clinical use showed that ECs produced isolated clumps and appeared as small spheroids with single point contact 54 . Synthetic vascular grafts coated with either antibodies or integrin-binding peptides have been developed for increasing cell adhesion to the inner lumen directly from the circulating blood stream 12, 55–57 .…”

Section: Discussionmentioning

confidence: 99%

“…Polymer 8g7 provides a superior substrate for attachment and proliferation of ECs where cells appeared homogenously distributed and exhibit the typical cobblestone monolayer morphology associated with high levels of attachment and enhanced cellular proliferation 54 . Moreover, decellularized tissue-engineered arteries can be stored and thus made readily available for patients similar to synthetic graft materials.…”

Section: Discussionmentioning

confidence: 99%

Poly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement

López‐Ruiz

Venkateswaran

Perán

et al. 2017

Sci Rep

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Decellularized vascular scaffolds are promising materials for vessel replacements. However, despite the natural origin of decellularized vessels, issues such as biomechanical incompatibility, immunogenicity risks and the hazards of thrombus formation, still need to be addressed. In this study, we coated decellularized vessels obtained from porcine carotid arteries with poly (ethylmethacrylate-co-diethylaminoethylacrylate) (8g7) with the purpose of improving endothelial coverage and minimizing platelet attachment while enhancing the mechanical properties of the decellularized vascular scaffolds. The polymer facilitated binding of endothelial cells (ECs) with high affinity and also induced endothelial cell capillary tube formation. In addition, platelets showed reduced adhesion on the polymer under flow conditions. Moreover, the coating of the decellularized arteries improved biomechanical properties by increasing its tensile strength and load. In addition, after 5 days in culture, ECs seeded on the luminal surface of 8g7-coated decellularized arteries showed good regeneration of the endothelium. Overall, this study shows that polymer coating of decellularized vessels provides a new strategy to improve re-endothelialization of vascular grafts, maintaining or enhancing mechanical properties while reducing the risk of thrombogenesis. These results could have potential applications in improving tissue-engineered vascular grafts for cardiovascular therapies with small caliber vessels.

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“…[91][92][93] The HUVECs cultured on decellularized SIS demonstrated higher proliferation and appeared more evenly spread with a cobblestone morphology, when compared with the ePTFE and Dacron alternatives. 44 Although these characteristics are indicative of better endothelialization, an important mechanism in preventing platelet adhesion, 17 both native and decellularized SIS grafts are prone to thrombosis. 91,94 Endothelialization of the lumen and a variety of surface treatments, including the immobilization of heparin and growth factors, have been employed to prevent thrombosis and improve the patency of SIS grafts.…”

Section: Two-dimensional Tissuementioning

confidence: 99%

“…38,39 When utilized in tissue engineering scaffolds, ECM encourages constructive remodeling and favorable clinical outcomes. 40,41 Specifically in vascular applications, collagen, 42,43 fibrin, 44 fibronectin, 45 laminin, 46,47 chondroitin sulfate, 48 and vascular endothelial growth factor (VEGF) 49 have been shown to encourage endothelialization. However, ECM components can also lead to platelet activation and thrombosis, [50][51][52] so it is important to consider the individual components, as well as the overall composition of the ECM.…”

Section: Introductionmentioning

confidence: 99%

Extracellular Matrix for Small-Diameter Vascular Grafts

Kimicata

Swamykumar

Fisher

2020

Tissue Engineering Part A

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To treat coronary heart disease, coronary artery bypass grafts are used to divert blood flow around blockages in the coronary arteries. Autologous grafts are the gold standard of care, but they are characterized by their lack of availability, low quality, and high failure rates. Alternatively, tissue-engineered small-diameter vascular grafts made from synthetic or natural polymers have not demonstrated adequate results to replace autologous grafts; synthetic grafts result in a loss of patency due to thrombosis and intimal hyperplasia, whereas scaffolds from natural polymers are generally unable to support the physiological conditions. Extracellular matrix (ECM) from a variety of sources, including cell-derived, 2D, and cannular tissues, has become an increasingly useful tool for this application. The current review examines the ECM-based methods that have recently been investigated in the field and comments on their viability for clinical applications.

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Comparing the endothelialisation of extracellular matrix bioscaffolds with coated synthetic vascular graft materials

Cited by 12 publications

References 46 publications

Design and Characterization of a Fluidic Device for the Evaluation of SIS-Based Vascular Grafts

Design and Characterization of a Fluidic Device for the Evaluation of SIS-Based Vascular Grafts

Poly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement

Extracellular Matrix for Small-Diameter Vascular Grafts

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