Comparing the efficacy of dietary α-tocopherol with that of dl-α-tocopheryl acetate, both either alone or in combination with ascorbic acid, on growth and stress resistance of angelfish, Pterophylum scalare, juveniles

Abstract

This study aimed to compare the efficacy of dietary a-tocopherol with that of DL-a-tocopheryl acetate, both either alone or in combination with vitamin C (ascorbic acid), on the growth performance, survival, and stress resistance of angelfish, Pterophylum scalare, juveniles. Juveniles were fed ad libitum for four weeks with Artemia enriched with no vitamins (control), vitamin C (T c ), a-tocopherol (T a ), DL-a-tocopheryl acetate (T DL ), a-tocopherol and vitamin C (T a+C ), and DL-a-tocopheryl acetate and vitamin C (T DL+C ). After four weeks, an osmotic stress test was performed using seawater (25 g/L) to evaluate juvenile's resistance to stress. Whole-body glucose and cortisol were used as stress indicators. At the end of the feeding trial, growth performance and survival of the juveniles fed vitamin-enriched Artemia were significantly (P \ 0.05) higher than for the control fish. Best performance was recorded for the T a+C group. Survival, however, was not significantly (P [ 0.05) different between the vitamin-fed groups. Osmotic stress significantly elevated the stress indicators, whole-body cortisol and glucose levels (P \ 0.05), highest and lowest values being observed in control and T a+C groups, respectively. Survival after osmotic stress of juveniles fed the T a+c diet was significantly higher (by 46.2%, P \ 0.001) than for controls. Results suggested that a-tocopherol has greater efficacy than DL-a-tocopheryl acetate and enriching Artemia with a-tocopherol and vitamin C together improves growth performance, survival, and stress resistance of angelfish juveniles.

The effects of vitamins C and E on the growth, tissue accumulation and prophylactic response to thermal and acidic stress of hybrid catfish

“…Similar synergistic effects of dietary vitamin C and E were demonstrated in other fish (Sealey & Gatlin ; Norouzitallab et al . ). Moreover, the present study showed that dietary vitamin C at 1000 mg kg −1 or dietary vitamin E at 62.5–125 mg kg −1 was sufficient to obtain maximal growth.…”

Dietary lycopene supplementation on Nile Tilapia (Oreochromis niloticus) juveniles submitted to confinement: effects on cortisol level and antioxidant response

“…Recent studies have reported that fish fed on antioxidant‐supplemented diets seemed to be better protected against stressors as seen in juveniles of Pterophylum scalare supplemented with α‐tocopherol, vitamin C and Artemia , a natural antioxidant (Norouzitallab et al 2008); other authors reported a similar effect in gilthead sea bream ( Spaurus aurata ) fed a vitamin‐C‐ supplemented diet (Henrique et al 1998). Other studies with rainbow trout ( Oncorhynchus mykiss ) have reported that the supplementation with antioxidant vitamins (vitamin E and C) reduced oxidative stress on fish red blood cells as well as promoted animal welfare (Trenzado et al 2009).…”

Biochemical and stress responses of rohu Labeo rohita and mrigal Cirrhinus mrigala in relation to acclimation temperatures

“…Vitamin C is well known for its anti‐oxidative property and has also been used as an immuno‐modulator in aquaculture (Cruz de Menezes et al , 2006; Norouzitallab et al , 2008). In the present study, the ascorbic acid contents in the brain and kidney tissues of both the species was reduced with increasing acclimation temperatures.…”