Comparing the diagnostic values of circulating microRNAs and cardiac troponin T in patients with acute myocardial infarction

Abstract

OBJECTIVE:Recent studies have shown that circulating microRNAs might be useful, novel biomarkers for the diagnosis of acute myocardial infarction. The aims of this study were to evaluate the expression of cardiac-specific miRNAs (miR-1, -133a, -208b, and -499) in patients with acute myocardial infarction and to compare the diagnostic values of these miRNAs with that of cardiac troponin T.METHODS:Sixty-seven plasma samples obtained from patients with acute myocardial infarction and 32 plasma specimens collected from healthy volunteers were analyzed in this study. The levels of cardiac-specific miRNAs (miR-1, -133a, -208b, and -499) were measured by quantitative reverse transcription-polymerase chain reaction, and the concentrations of plasma cardiac troponin T were measured using electrochemiluminescence-based methods and an Elecsys 2010 Immunoassay Analyzer.RESULTS:The levels of plasma miR-1, -133a, -208b, and -499 were significantly higher in acute myocardial infarction patients (all p<0.001) than in healthy volunteers. The expression of the cardiac-specific miRNAs in acute myocardial infarction patients decreased to close to the baseline levels at the time of hospital discharge (all p>0.05). There were no correlations between the levels of the four circulating miRNAs and the clinical characteristics of the study population (all p>0.05). Furthermore, receiver operating characteristic curve analyses showed that the four plasma miRNAs were not superior to cardiac troponin T for the diagnosis of acute myocardial infarction (all p>0.05).CONCLUSION:Our results demonstrate that circulating miR-1, -133a, -208b, and -499 may be useful biomarkers in acute myocardial infarction patients but that these miRNAs are not superior to cardiac troponin T for the diagnosis of acute myocardial infarction.

Circulating miR-499 as a potential biomarker for acute myocardial infarction

“…In the elderly patients with acute NSTEMI, a study showed that circulating miRNA-499-5p had better accuracy in the diagnosis compared with cTnT (23). In line with the above findings, another recent study demonstrated that plasma level of miRNA-499-5p was increased distinctly in AMI patients, suggesting that circulating miR-499-5p could be used for the evaluation of mortality risk (24).…”

The role of microRNAs in coronary artery disease: From pathophysiology to diagnosis and treatment

“…Li et al concluded that the combination of the aforementioned four miRNAs may be useful but is not superior to cTnT for AMI diagnosis [95]. Surprisingly, miR-208a and miR-499a do not belong to the 20 most enriched miRNAs in the blood of MI-patients, as measured by Meder et al in whole blood-microarrays.…”

Relationship of Microalbuminurea in Non-Diabetic and Non-Hypertensive Patients with Acute Myocardial Infarction

“…Posterior wall MI,LWMI, NSTEMI, a VR MI with percentage of 1.5%, 2.0%, 35.0% and 1.5% respectively. In the study of Singh et al [15] reported that troponin T test was positive in the 100% cases with acute MI. According to AlaHussain Abbase Haider [16], level of CK-MB divided into two groups, group with elevated CK-MB patients 35.7% and group with normal CK-MB patients 64.3%.…”