Comparing formulations for a mixed-microbial biopesticide with Bacillus thuringiensis var. kurstaki and Beauveria bassiana blastospores

Sayed, A. M. M.; Behle, Robert W.

doi:10.1080/03235408.2017.1372942

Cited by 10 publications

(8 citation statements)

References 34 publications

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“…219. 53 Organisms were exposed to pre-established sublethal concentrations of Btk [8,20,50 and 126 μg a.i./L, plus control treatment (ASTM hard water only)] and Bb (0.07, 0.14, 0.28, 0.56 μg a.i./L, plus control treatment) for a period of 28 days. First-instar larvae (2 days old) were introduced into 200 mL glass vials containing a 1.5 cm layer of inorganic sediment (< 1 mm), previously burnt at 500 ∘ C for 5 h, and 150 mL of aerated experimental solutions.…”

Section: Life History Responsesmentioning

confidence: 99%

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Toxicity of microbial insecticides toward the non‐target freshwater insect Chironomus xanthus

Dornelas

Sarmento

Silva

et al. 2019

Pest Management Science

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BACKGROUND Commercial formulations based on Bacillus thuringiensis subs. kurstaki (Btk) and Beauveria bassiana (Bb) are commonly used microbial insecticides in Brazil and other tropical regions. However, and despite being considered environmentally friendly, their use generates concerns regarding possible adverse ecological effects in freshwater ecosystems. Here, we evaluate the effects of these bioinsecticides on the tropical aquatic dipteran Chironomus xanthus under laboratory conditions. RESULTS After laboratory exposures to these compounds 48‐h median lethal concentration (LC50) values of 1534 μg a.i./L for Btk and of 6.35 μg a.i./L for Bb were estimated. Chronic assays revealed different sublethal effects: Btk‐based bioinsecticide exposure reduced C. xanthus growth [lowest observed effect concentration (LOEC) was 126 μg a.i./L for head width], decreased emergence rate (LOEC = 8 μg a.i./L) and increased immunological response (LOEC = 50 μg a.i./L) measured as total hemocyte count in larvae hemolymph. Exposure to low concentrations of Bb‐based insecticide also reduced C. xanthus growth (LOEC = 0.07 μg a.i./L for larvae body length measurements), and emergence rate (LOEC = 0.28 μg a.i./L), despite no clear effects on the total hemocyte counts. CONCLUSION Our results suggest that low concentrations of Btk and Bb bioinsecticides are toxic to C. xanthus. Given their widespread use and occurrence in tropical freshwater systems, research is needed to evaluate the potential effects of these compounds concerning natural freshwater insect communities and ecosystem functioning. © 2019 Society of Chemical Industry

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Section: Life History Responsesmentioning

confidence: 99%

“…Commercial formulations based on the entomopathogens Bacillus thuringiensis Berliner and Beauveria bassiana (Balsamo) Vuillemin are two of the most widely used microbial insecticides . B. thuringiensis is a Gram‐positive aerobic bacterium that occurs naturally in soil, water, air and plants in their vegetative form .…”

Section: Introductionmentioning

confidence: 99%

Toxicity of microbial insecticides toward the non‐target freshwater insect Chironomus xanthus

Dornelas

Sarmento

Silva

et al. 2019

Pest Management Science

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“…Another important issue that should be addressed is the determination of alkaline conditions of spray-dried biochar combined with three different entomopathogens: a baculovirus (AgipMNPV), bacterium (Bt) and fungus (Bb). In our previous research (Sayed & Behle, 2017b), differences in mixing activity were observed that may be related to the pH of biochar. The result was somewhat unexpected and needed to be studied before biochar is used for production and formulation of biopesticides.…”

Section: Introductionmentioning

confidence: 88%

Insecticidal activity of bio-oils and biochar as pyrolysis products and their combination with microbial agents against agrotis ipsilon (Lepidoptera: Noctuidae)

et al. 2018

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Pyrolysis technology for producing biochar and bio-oils can be used as a potential alternative to make biopesticides, which are urgently needed in integrated pest management (IPM). Insecticidal activity of three components of bio-oils: aqueous, organic and their mixture, was evaluated individually and with three different entomopathogens: the baculovirus Agrotis ipsilon nucleopolyhedrovirus (AgipMNPV), bacterium Bacillus thuringensis var. kurstaki (Bt) and fungus Beauveria bassiana (Bb) against black cutworm, Agrotis ipsilon (Hufnagel). The effect of alkaline conditions of spray-dried biochar was studied simultaneously with the microbial pathogens. Our bioassay results indicated that the organic bio-oil phase was the most active, causing 100% mortality after 24 h, when the median lethal toxicity values LC50s (mg/ml) were found. However, the bio-oil fractions applied alone tended to cause higher mortality of the exposed larvae than did either mix with the microbial agents. Also, the results revealed that maximum mortalities were found in spray-dried formulations made with biochar at pH 7.1. It was concluded that pyrolysis oils are effective insecticides, and biochar could be a useful additive in production and formulation of biopesticides. This interesting finding further promotes the use of pyrolysis bio-oils and biochar compounds as eco-friendly alternatives to replace conventional pesticides.

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“…Compared with liquid agents, solid ones have many advantages, such as easy storage and transport, a longer action time, the protection of degrading bacteria from environmental influences, and the provision of a suitable microenvironment for bacterial survival ( Ruan et al, 2018 ; Zhu et al, 2021 ). There have been some reports on the commercialization of high-efficiency degrading strains for the remediation of soil and water ( Sayed and Behle, 2017a ; Mohd Yusof et al, 2019 ). The solid agents prepared by the cyclic aromatic hydrocarbon-degrading bacteria greatly improved the degradation of the organic pollutant pyrene in soil ( Wang et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Development of solid agents of the diphenyl ether herbicide degrading bacterium Bacillus sp. Za based on a mixed organic fertilizer carrier

Zhao

Tian

et al. 2022

Front. Microbiol.

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The long-term and widespread use of diphenyl ether herbicides has caused serious soil residue problems and threatens the agricultural ecological environment. The development of biodegrading agents using high-efficiency degrading strains as pesticide residue remediation materials has been widely recognized. In this study, the strain Bacillus sp. Za was used to prepare solid agents for the remediation of diphenyl ether herbicides-contaminated soil. The ratio of organic fertilizer was 1:3 (pig manure: cow dung), the inoculum amount of Za was 10%, the application amount of solid agents was 7%, and the application mode was mixed application, all of which were the most suitable conditions for solid agents. After the solid agents were stored for 120 days, the amount of Za remained above 108 CFU/g. The degradation rates of the solid agents for lactofen, bifenox, fluoroglycofen, and fomesafen in soil reached 87.40, 82.40, 78.20, and 65.20%, respectively, on the 7th day. The application of solid agents alleviated the toxic effect of lactofen residues on maize seedlings. A confocal laser scanning microscope (CLSM) was used to observe the colonization of Za-gfp on the surface of maize roots treated in the solid agents, and Za-gfp mainly colonized the elongation zone and the mature area of maize root tips, and the colonization time exceeded 21 days. High-throughput sequencing analysis of soil community structural changes in CK, J (solid agents), Y (lactofen), and JY (solid agents + lactofen) groups showed that the addition of solid agents could restore the bacterial community structure in the rhizosphere soil of maize seedlings. The development of solid agents can facilitate the remediation of soil contaminated with diphenyl ether herbicide residues and improve the technical level of the microbial degradation of pesticide residues.

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Comparing formulations for a mixed-microbial biopesticide with Bacillus thuringiensis var. kurstaki and Beauveria bassiana blastospores

Cited by 10 publications

References 34 publications

Toxicity of microbial insecticides toward the non‐target freshwater insect Chironomus xanthus

Toxicity of microbial insecticides toward the non‐target freshwater insect Chironomus xanthus

Insecticidal activity of bio-oils and biochar as pyrolysis products and their combination with microbial agents against agrotis ipsilon (Lepidoptera: Noctuidae)

Development of solid agents of the diphenyl ether herbicide degrading bacterium Bacillus sp. Za based on a mixed organic fertilizer carrier

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