Comparing extraction methods for the determination of tocopherols and tocotrienols in seeds and germinating seeds of soybean transformed with OsHGGT

Lee, Yu Young; Park, Hyang Mi; Lee, Choon-Ki; Kim, Sun Lim; Hwang, Tae-Young; Choi, Man Soo; Kwon, Young-Up; Kim, Wook Han; Kim, Si Ju; Lee, Sang Chul; Kim, Yul Ho

doi:10.1016/j.jfca.2012.03.010

Cited by 19 publications

(13 citation statements)

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“…Previous studies have suggested that optimal methods should be used for the comparison of various antioxidant activities from different types of crop materials. Extraction methods, analytical conditions and the types of sample could affect the concentration of valuable minor functional components such as tocopherols, and the antioxidant activity levels . The content of total vitamin E by rapid Soxhlet extraction in seed and direct extraction in germinating soybean were approximately 37% and 16% greater than the amount obtained by the saponification method, which has been widely used .…”

Section: Resultsmentioning

confidence: 99%

“…Extraction methods, analytical conditions and the types of sample could affect the concentration of valuable minor functional components such as tocopherols, and the antioxidant activity levels . The content of total vitamin E by rapid Soxhlet extraction in seed and direct extraction in germinating soybean were approximately 37% and 16% greater than the amount obtained by the saponification method, which has been widely used . Moreover, the reversed‐phase column for analysis of tocopherols could not detect separated forms of β‐, γ‐ tocopherols and tocotrienols, whereas a normal‐phase column could detected eight different vitamin E isomers (α‐, β‐, γ‐, δ‐ tocopherols and tocotrienols); thus direct comparisons of the results of different studies may be seen to be inappropriate.…”

Section: Resultsmentioning

confidence: 99%

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A correlation between tocopherol content and antioxidant activity in seeds and germinating seeds of soybean cultivars

et al. 2014

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

A correlation between tocopherol content and antioxidant activity in seeds and germinating seeds of soybean cultivars

et al. 2014

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“…To avoid contamination, the healthy grains were disinfected in a mixture of 5% sodium hypochlorite and 70% alcohol for 5 min. The disinfected grains were rinsed with sterile distilled water several times (Lee et al., 2012). Thereafter, 30 disinfected grains were placed on a sterile filter paper in the petri dishes that were kept in a growth chamber for 7 d at 25 °C.…”

Section: Methodsmentioning

confidence: 99%

Foliar applied zinc increases yield, zinc concentration, and germination in wheat genotypes

et al. 2020

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Human malnutrition associated with zinc (Zn) deficiency is a growing problem in many areas. This study was conducted to assess wheat (Triticum aestivum L.) genotypic variation of grain Zn concentration, grain germination, and grain yield in response to foliar application of Zn. Twenty-four wheat genotypes, including several recombinant inbred lines (RILs) and commercial cultivars, were assessed in two separate experiments; one experiment sprayed with ZnSO 4 ⋅7H 2 O dissolved in water at wheat heading growth stage and one without applying Zn foliar treatment. The range for grain (25.1-63.1) and endosperm (1.2-61.6 mg kg −1 ) Zn concentrations were different between the two Zn treatments. Significant (p = .05) genotype × Zn treatment interactions showed that some genotypes were more responsive to Zn applications than others. The application of Zn increased the Zn concentration in the endosperm in 18 out of the 24 genotypes. For grain, Zn application increased the concentration in the grain in 21 out of the 24 genotypes. Grain yield and germination were increased in the Zn treated cultivars compared with non-treated. In conclusion, foliar application of Zn improved traits in wheat, and identified genetic variation lays the foundation for genotype selection with respect to higher Zn concentration.Abbreviations: CCA, canonical correlation analysis; CIMMYT, international maize and wheat improvement center; DTZ, diphenyl-thiocarbazone; GCV, genotypic coefficients of variations; GE, germination energy; GS, germination speed; GV, germination value; MTG, mean time of germination; PCA, principal component analysis; PCV, phenotypic coefficients of variations; RCBD, randomized complete block design; RIL, recombinant inbred line; TPN, total pixel number.

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“…2.2.3.6 Determination of RSO tocopherols RSO Samples were saponified according to Lee et al (2012). HPLC analysis was carried out using Agilent Technologies 1100 series liquid chromatograph equipped with an auto-sampler and a diode-array detector.…”

Section: Calculated Oxidizability Value (Cox)mentioning

confidence: 99%

Evaluation of green extraction methods on the chemical and nutritional aspects of roselle seed (Hibiscus sabdariffa L.) oil

Naeem

Zahran

Hassanein

2019

OCL

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Roselle is one of the valuable plants grown in some regions of Egypt, which is used to make juices or as natural food color additive. Roselle seeds are waste, which can be used as a source of nontraditional oil, nutritious or functional compound. The evaluation of green extraction methods including supercritical CO 2 (SC-CO 2 ), screw, hydraulic press comparing to traditional method on oil yield, total phenolics, antioxidant activity (DPPH) and oxidative stability of roselle seeds oil were investigated. Fatty acid composition and tocopherol of the oil were also determined. The results showed that the roselle seeds oil extracted by solvent gave the highest oil content and extraction rate (17.98 and 98.34%, respectively) and the lowest peroxide and acid value. SC-CO 2 gave the higher content of alpha, gamma, and delta tocopherol comparing to the other extraction methods. Fatty acid showed that linoleic acid, an essential fatty acid, was dominant followed by oleic and palmitic acids. These fatty acids were higher amounts in oil extracted by SC-CO 2 , followed by cold-press comparing to solvent extraction. The maximum stability (20 h), higher TPC (22.18 mg GAE/g) and antioxidant activity (DPPH, 65.15) were observed in oil extracted by SC-CO 2 followed by hydraulic-press, solvent extraction and finally screw-press. The results revealed that SC-CO 2 method is more efficient in determination of total tocopherol, oxidative stability, TPC and radical scavenging activity than the other extraction methods. Cold press extraction methods gave higher oil yield than SC-CO 2 and more economically than other methods.Keywords: roselle seed oil / green extraction methods / oxidative stability / non-traditional oil / antioxidant activity Résumé -Évaluation de méthodes d'extraction vertes sur les caractéristiques chimiques et nutritionnelles de l'huile de graines d'Hibiscus (Hibiscus sabdariffa L.). L'hibiscus constitue l'une des plantes d'intérêt cultivées dans certaines régions d'Égypte, utilisée pour fabriquer des jus ou comme source de colorant alimentaire. Les graines d'hibiscus étant inutilisées dans ces filières, elles peuvent être employées comme source d'huile non traditionnelle, de composés nutritionnels ou fonctionnels. Les méthodes d'extraction vertes, parmi lesquelles l'extraction au CO 2 supercritique (SC-CO 2 ), la presse à vis et la presse hydraulique, ont été comparées à la méthode traditionnelle en termes de rendement en huile, de teneurs en composés phénoliques totaux, d'activité anti-oxydante (DPPH) et de stabilité à l'oxydation de l'huile de graines d'hibiscus. Les résultats montrent que l'huile de graines d'hibiscus extraite par solvant donne les plus grands rendement et taux d'extraction (17,98 et 98,34 %, respectivement), et les plus faibles indices d'acide et de peroxyde. L'extraction au SC-CO 2 conduit aux teneurs les plus élevées en tocophérols alpha, gamma et delta par rapport aux autres méthodes d'extraction. En termes de profil en acides gras, l'acide linoléique, un acide gras essentiel, se révèle prédom...

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Comparing extraction methods for the determination of tocopherols and tocotrienols in seeds and germinating seeds of soybean transformed with OsHGGT

Cited by 19 publications

References 50 publications

A correlation between tocopherol content and antioxidant activity in seeds and germinating seeds of soybean cultivars

A correlation between tocopherol content and antioxidant activity in seeds and germinating seeds of soybean cultivars

Foliar applied zinc increases yield, zinc concentration, and germination in wheat genotypes

Evaluation of green extraction methods on the chemical and nutritional aspects of roselle seed (Hibiscus sabdariffa L.) oil

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