A correlation between tocopherol content and antioxidant activity in seeds and germinating seeds of soybean cultivars

Lee, Yu Young; Park, Hyang Mi; Hwang, Tae Sook; Kim, Sun Lim; Kim, Mi Jung; Lee, Seuk Ki; Seo, Min; Kim, Kee Jong; Kwon, Young-Up; Lee, Sang Chul; Kim, Yul Ho

doi:10.1002/jsfa.6963

Cited by 18 publications

(13 citation statements)

References 34 publications

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“…It is plausible to conclude that this delay in quantifying the tocopherol levels would lead to lower and/or unpredictable levels, as past results suggested that soybean seed storage caused major tocopherol losses (Wohleser, 2007). Degradation of tocopherols during seed storage reported in another study, however, found that tocopherol levels and ratios remain consistent for up to 6 mo of storage (Lee et al, 2015). The 2010 soybean seeds were subjected to HPLC analysis immediately after harvest in November and December of 2010.…”

Section: Discussionmentioning

confidence: 98%

“…Soybean seed contains high levels of tocopherols, which in their natural form provide benefits against many human health conditions such as lung cancer, chronic inflammation, and cardiovascular disease. The benefits can be attributed to the tocopherols' strong antioxidative properties, which protect cells from reactive oxygen species and also help by impacting cellular responses to oxidative stress (Lee et al, 2015). The main purpose of tocopherols in soybean seed is to protect the lipids from lipid peroxidation (Evans et al, 2002).Agronomic practices and location and year effects on tocopherols are very important in understanding the influence that they have in causing tocopherol profile differences.…”

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confidence: 99%

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Effect of Genotype, Environment, and Genotype × Environment Interaction on Tocopherol Accumulation in Soybean Seed

2016

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Soybean seeds [Glycine max (L.) Merrill] are a major source of tocopherols that provide many human health benefits including decrease in lung cancer risk and osteoporosis. The objectives of this study were: (i) to determine the impact of genotype, environment, and genotype × environment on soybean seed tocopherols and (ii) to evaluate relationships between agronomic traits and tocopherols. Seventy‐nine recombinant inbred lines (RILs) from the cross OAC Bayfield × OAC Shire were grown in three field locations in southern Ontario, Canada, in 2009 and 2010. Agronomic and seed quality data were collected in each location. Significant differences (P < 0.001) for the α‐, γ‐, δ‐, and total tocopherol concentrations were found in the seed of RILs using combined ANOVA for 2009 and 2010. Of the two parental lines, OAC Bayfield accumulated more total tocopherol at most environments. Genotype, location, and year differences were significant as well as genotype × location, location × year, and line × year interactions. Broad‐sense heritability estimates were 0.38 for α‐, 0.47 for γ‐, and 0.35 for δ‐tocopherol. None of the agronomic traits were consistently correlated with any of the tocopherol components; however, oil and protein concentration were correlated to some tocopherols in some of the environments. The wide range in values for α‐, γ‐, δ‐, and total tocopherols among the RIL population that exceeded both parents provided evidence for transgressive segregation. Our results suggest that soybean producers should take locations and genotypes into account when growing soybean for enhanced tocopherol production.

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Section: Discussionmentioning

confidence: 98%

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confidence: 99%

Effect of Genotype, Environment, and Genotype × Environment Interaction on Tocopherol Accumulation in Soybean Seed

2016

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“…Tocopherols and carotenoids are well known for their antioxidant functions in prevention of various chronic diseases (Agarwal & Rao, 2000; Valentin & Qi, 2005). We believe that these fat‐soluble micronutrients in Cheongja#3 create a synergistic effect with the antioxidant functions of black soybeans in Cheongja#3 soybean extract than that is not found in other soybean varieties (Lee, Park, et al., 2015). …”

Section: Discussionmentioning

confidence: 99%

“…Their biological activities including antioxidative and anti-inflammatory effects help to reduce the risk of cancer and metabolic disorders (Ganesan & Xu, 2017). Cheongja#3 is a cultivar of black soybean which is well known to contain high amounts of anthocyanins as well as tocopherols (Lee et al, 2009;Lee, Park, et al, 2015). Several previous studies have shown that Cheongja#3 had antiobesity effects in cells, mice, and humans (Jeon, Lee, & Cheon, 2015;Kim et al, 2015;Lee, Sorn, Park, & Park, 2016), as well as neuroprotective effects (Bhuiyan, Kim, Ha, Kim, & Cho, 2012;Kim, Chung, et al, 2012).…”

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confidence: 99%

Black soybeans protect human keratinocytes from oxidative stress‐induced cell death

Yoon

Lee

Song

et al. 2018

Food Science & Nutrition

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Black soybeans are functional foods containing a variety of bioactives such as isoflavones, carotenoids, tocopherols, phenolic acid as well as anthocyanins. Here, we examined whether Cheongja#3 black soybean extract has a protective effect on oxidative stress‐induced cell death in human keratinocytes HaCaT. First, we identified fat‐soluble bioactives in three varieties of soybean extracts (Saedanbaek, Daechan, and Cheongja#3). In particular, black soybean Cheongja#3 had high amounts of lutein than other varieties. We demonstrated that Cheongja#3 extract reduced intracellular reactive oxygen species levels in HaCaT cells. Furthermore, Cheongja#3 protected cells from hydrogen peroxide (H2O2)‐induced oxidative stress and triggered cell death determined by cell viabilities and apoptotic caspase activities. Next, we identified the underlying mechanism is due to increased Nrf2 antioxidant system by Cheongja#3, thus increasing the expression of heme oxygenases (HO)‐1. These results indicated that Cheongja#3 soybean extract has protective role against oxidative stress by upregulating the Nrf‐2 antioxidant system in human keratinocyte HaCaT cells.

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“…Then, we identified lutein (111.8 μg/100 g), γ-tocotrienol (757.7 μg/100 g) and γ-tocopherol (4071.4 μg/100 g) in SBx. These bioactive components are well known antioxidant components, thus leading to various biological functions for health benefits [ 36 , 37 ]. Further studies are necessary with regard to determination of functional groups or their purity in these extracts to understand the mechanism of actions for their antioxidant activities.…”

Section: Resultsmentioning

confidence: 99%

Enhanced Antioxidant Activity of Bioactives in Colored Grains by Nano-Carriers in Human Lens Epithelial Cells

Lee

Yoon

et al. 2018

Molecules

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The use of phytochemicals for preventing chronic diseases associated with oxidative stress such as cataracts is hindered by their low bioavailability. The effects of nano-carriers on the antioxidant activities of extracts of black rice with giant embryo (BRGEx) and soybeans (SBx) have been determined in human lens epithelial B3 cells. Scanning (SEM) and transmission electron microscopy (TEM) demonstrated that rGO (reduced graphene oxide) has a flat surface unlike GO (graphene oxide), which has a distinctive wrinkled structure with defects. UPLC analysis revealed 41.9 μg/100 g of γ-oryzanols in water extract of BRGE, and 111.8 μg /100 g of lutein, 757.7 μg/100 g of γ-tocotrienol, 4071.4 μg/100 g of γ-tocopherol in 40% ethanol extract of soybeans, respectively. Even though a low concentration of BRGEx alone did not show any antioxidant activity in B3 cells, co-treatment of BRGEx with rGO together substantially reduced hydrogen peroxide and methylglyoxal-induced DNA damage, as determined by phosphorylated γH2AX. In addition, SBx with rGO also attenuated DNA damage. Furthermore, intracellular reactive oxygen species were significantly decreased by combining extracts of these colored grains with rGO. These results suggest a potential application of nanocarriers for enhancing the bioavailability of phytochemicals.

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A correlation between tocopherol content and antioxidant activity in seeds and germinating seeds of soybean cultivars

Abstract: Total tocopherol content was higher in soybean landraces as compared with modern cultivars developed by cross-breeding. These results suggest that soybean breeding is necessary to increase tocopherol levels.

Cited by 18 publications

References 34 publications

Effect of Genotype, Environment, and Genotype × Environment Interaction on Tocopherol Accumulation in Soybean Seed

Effect of Genotype, Environment, and Genotype × Environment Interaction on Tocopherol Accumulation in Soybean Seed

Black soybeans protect human keratinocytes from oxidative stress‐induced cell death

Enhanced Antioxidant Activity of Bioactives in Colored Grains by Nano-Carriers in Human Lens Epithelial Cells

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