Comparing different physical factors on serum TNF-α levels, chondrocyte apoptosis, caspase-3 and caspase-8 expression in osteoarthritis of the knee in rabbits

Guo, Hua; Luo, Qiong; Zhang, Jinlong; Lin, Haidan; Xia, Lu; He, Chengqi

doi:10.1016/j.jbspin.2011.01.009

Cited by 45 publications

(33 citation statements)

References 29 publications

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“…To this end, our study revealed a close relationship between the OA phenotype and high expression levels of MMP-13 and ADAMTS-5. It has been reported that apoptosis represents a principal mechanism of chondrocyte degeneration during OA [40][41][42]. Likewise, we found that LiCl-induced β-catenin signalling in adult chondrocytes led to increased apoptosis.…”

Section: Discussionsupporting

confidence: 77%

Dual function of β-catenin in articular cartilage growth and degeneration at different stages of postnatal cartilage development

Niu

Wang

Xinghong

et al. 2011

International Orthopaedics (SICOT)

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Purpose The objective of this study was to determine the role of β-catenin in normal postnatal articular cartilage growth and degeneration. Methods We investigated β-catenin gene and protein expression in hip cartilage cells of normal Wistar rats at two, four, six and eight weeks of age by using reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry. Primary articular chondrocytes from eight week old rats were cultured and treated with LiCl for activation of β-catenin. Collagen X and matrix metalloproteinase 13 (MMP-13) were detected by quantitative RT-PCR and immunofluorescence. A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-4 and 5 were detected by quantitative RT-PCR, and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) was used for detecting cell apoptosis. Results The highest levels of β-catenin expressions were detected in two week old rats, after which a steady decline was observed over the remaining period of observation (p< 0.05). When primary articular chondrocytes from eight week old rats were treated with LiCl, β-catenin mRNA and protein were induced (p<0.05). Moreover, LiCl-activated β-catenin in chondrocytes was associated with significant concomitant increases in mRNA expression of collagen X and the MMP-13 encoding collagenase 3. Significantly increased mRNA expression of ADAMTS-5 was also seen in primary chondrocytes from eight week old rats after LiCl treatment (p<0.05). The effect was specific to ADAMTS-5 since ADAMTS-4, which has similar proteolytic activity but different aggrecanase activity, was unaffected. Finally, TUNEL staining revealed that LiCl-activated β-catenin signalling led to increased cell apoptotic events in chondrocytes (p<0.05). Conclusions Our findings suggest that normal spatiotemporal patterns and degrees of Wnt/β-catenin signalling are needed to maintain postnatal articular cartilage growth and function. In the early stages of cartilage development, activation of β-catenin signalling is necessary for articular cartilage growth, while in adult cartilage it leads to degeneration and osteoarthritic-like chondrocytes.

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Section: Discussionsupporting

confidence: 77%

Dual function of β-catenin in articular cartilage growth and degeneration at different stages of postnatal cartilage development

Niu

Wang

Xinghong

et al. 2011

International Orthopaedics (SICOT)

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“…Increased expression of inflammatory markers was found in the control animals, mainly at 8 weeks, indicating the progression of the course of the disease and the development of the inflammatory process. Laser therapy was able to modulate the expression of MMP-13, which corroborates with many authors who affirm that LLLT has been found to produce anti-inflammatory effects in a variety of disorders [48]. The anti-inflammatory effects of LLLT on degenerative processes has been demonstrated by a number of several authors who observed a decrease in prostaglandin E2 and TNF expression after LLLT in OA experimental models [48].…”

Section: Discussionsupporting

confidence: 81%

Effects of low-level laser therapy on cartilage repair in an experimental model of osteoarthritis

Santos

Oliveira

Fernandes

et al. 2014

Photonics &Amp; Lasers in Medicine

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Objective: The aim of this study was to evaluate the effects of low level laser therapy (LLLT) on the degenerative process in the articular cartilage after an anterior cruciate ligament transection (ACLT) model in rats. Methods: Eighty male rats (Wistar) were divided into four groups: 1.) intact control group (CG), 2.) injured control group (ICG), 3.) injured laser-treated group at 10 J/cm 2 (L10) and 4.) injured laser-treated group at 50 J/cm 2 (L50). Animals were divided into 2 subgroups, with different periods of sacrifice (5 and 8 weeks post-surgery). The ACLT was used to induce osteoarthritis (OA) in the knees of the rats. LLLT started 2 weeks after the surgery and it was performed for 15 and 30 sessions, respectively using a 685-nm laser, at 10 and 50 J/cm 2 . Qualitative and semiquantitative histologic, morphometric and immunohistochemistry analyses were performed. Results: Initial signs of tissue degradation could be observed 5 weeks post-ACLT, evidenced by the decrease of proteoglycan concentration and increase in cartilage thickness of the ICG. After 8 weeks post-surgery, analysis showed a progression of the degenerative processes in the ICG revealed by the increased cellularity and higher TNF-α, IL1-β and MMP-13 immunoexpression. LLLT was able to modulate some of the aspects relating to the degradative process, such as biomodulation of the number of chondrocyte proliferation, prevention of proteoglycan loss, and decrease of MMP-13 immunoexpression. Conclusion: This study showed that the 685-nm laser irradiation, especially at 10 J/cm 2 , prevented features related to the articular degenerative process in the knees of rats. ZusammenfassungZiel: Das Ziel dieser Studie war es, die Effekte der LowLevel-Lasertherapie (LLLT) auf den degenerativen Prozess im Gelenkknorpel von Ratten nach vorderer Kreuzbanddurchtrennung zu evaluieren. Methoden: Achtzig männliche Wistar-Ratten wurden in vier Versuchsgruppen unterteilt: 1.) intakte Kontrollgruppe (CG), 2.) verletzte Kontrollgruppe (ICG), 3.) verletzte Laser-behandelte Gruppe bei 10 J/cm 2 (L10) und 4.) verletzte Laser-behandelte Gruppe bei 50 J/cm 2 (L50). Die Tiere wurden in zwei Untergruppen aufgeteilt und entweder 5 oder 8 Wochen nach der Operation eingeschläfert. Die vordere Kreuzbanddurchtrennung wurde verwendet, um in den Kniegelenken der Ratten Osteoarthritis (OA) zu induzieren. Die LLLT begann 2 Wochen nach der Operation und wurde für 15 bzw. 30 Sitzungen bei 10 und 50 J/cm 2 mit einem 685 nm-Laser durchgeführt. Qualitative und semi-quantitative histologische, morphometrische und immunhistochemische Analysen wurden durchgeführt. Ergebnisse: Erste Anzeichen von Gewebeabbau wurden 5 Wochen nach der vorderen Kreuzbanddurchtrennung beobachtet und durch die Abnahme der ProteoglycanKonzentration und die Erhöhung der Knorpeldicke in der verletzten Kontrollgruppe (ICG) belegt. Acht Wochen nach der Operation zeigte sich in der ICG ein Fortschreiten der degenerativen Prozesse durch eine erhöhte Zellularität und eine höhere TNF-α-, IL1-β-und MMP-13-Immunexpression....

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“…Guo and colleagues performed a study that evaluated and compared the effects of millimeter waves, pulsed electromagnetic fields, ultrasound, LLLT, and short-wave diathermy on the serum levels of TNFα, chondrocyte apoptosis, caspase-3 and caspase-8, in an experimental model of OA in rabbit knees, and they suggested that their findings may shed light on the efficacy of various physical treatments in OA management [34]. The same authors suggested that LLLT was not the ideal treatment modality for OA.…”

Section: Discussionmentioning

confidence: 99%

Effect of low-level laser therapy on the expression of inflammatory mediators and on neutrophils and macrophages in acute joint inflammation

et al. 2013

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IntroductionInflammation of the synovial membrane plays an important role in the pathophysiology of osteoarthritis (OA). The synovial tissue of patients with initial OA is characterized by infiltration of mononuclear cells and production of proinflammatory cytokines and other mediators of joint injury. The objective was to evaluate the effect of low-level laser therapy (LLLT) operating at 50 mW and 100 mW on joint inflammation in rats induced by papain, through histopathological analysis, differential counts of inflammatory cells (macrophages and neutrophils), as well as gene expression of interleukin 1-beta and 6 (IL-1β and IL-6), and protein expression of tumor necrosis factor alpha (TNFα).MethodsMale Wistar rats (n = 60) were randomly divided into four groups of 15 animals, namely: a negative control group; an inflammation injury positive control group; a 50 mW LLLT group, subjected to injury and treated with 50 mW LLLT; and a 100 mW LLLT group, subjected to injury and treated with 100 mW LLLT. The animals were subject to joint inflammation (papain solution, 4%) and then treated with LLLT (808 nm, 4 J, 142.4 J/cm2, spot size 0.028 for both groups). On the day of euthanasia, articular lavage was collected and immediately centrifuged; the supernatant was saved for analysis of expression of TNFα protein by enzyme-linked immunosorbent assay and expression of IL-1β and IL-6 mRNA by real-time polymerase chain reaction. A histologic examination of joint tissue was also performed. For the statistical analysis, analysis of variance with Tukey's post-hoc test was used for comparisons between each group. All data are expressed as mean values and standard deviation, with P < 0.05.ResultsLaser treatment with 50 mW was more efficient than 100 mW in reducing cellular inflammation, and decreased the expression of IL-1β and IL-6. However, the 100 mW treatment led to a higher reduction of TNFα compared with the 50 mW treatment.ConclusionsLLLT with 50 mW was more efficient in modulating inflammatory mediators (IL-1β, IL-6) and inflammatory cells (macrophages and neutrophils), which correlated with the histology that showed a reduction in the inflammatory process.

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Comparing different physical factors on serum TNF-α levels, chondrocyte apoptosis, caspase-3 and caspase-8 expression in osteoarthritis of the knee in rabbits

Cited by 45 publications

References 29 publications

Dual function of β-catenin in articular cartilage growth and degeneration at different stages of postnatal cartilage development

Dual function of β-catenin in articular cartilage growth and degeneration at different stages of postnatal cartilage development

Effects of low-level laser therapy on cartilage repair in an experimental model of osteoarthritis

Effect of low-level laser therapy on the expression of inflammatory mediators and on neutrophils and macrophages in acute joint inflammation

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