Comparing C60+ and (H2O)n+ clusters for mouse brain tissue analysis

Razo, Irma Berrueta; Sheraz, Sadia; Henderson, Alex; Lockyer, Nicholas P.; Vickerman, John C.

doi:10.1002/sia.5597

Cited by 4 publications

(1 citation statement)

References 17 publications

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“…8,9,[18][19][20] The results indicate that in the singlecomponent samples, the ionization of both molecules is enhanced with the (H 2 O) n þ ion beam, particularly with larger water clusters (Fig. 2).…”

Section: A Analysis Of Single-component Samplesmentioning

confidence: 90%

Matrix effects in biological SIMS using cluster ion beams of different chemical composition

2016

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The influence of the matrix effect on secondary ion yield presents a very significant challenge in quantitative secondary ion mass spectrometry (SIMS) analysis, for example, in determining the relative concentrations of metabolites that characterize normal biological activities or disease progression. Not only the sample itself but also the choice of primary ion beam may influence the extent of ionization suppression/enhancement due to the local chemical environment. In this study, an assessment of ionization matrix effects was carried out on model systems using C60+, Arn+, and (H2O)n+ cluster ion beams. The analytes are pure and binary mixtures of amino acids arginine and histidine biological standards. Ion beams of 20 keV were compared with a range of cluster sizes n = 1000–10 000. The component secondary ion yields were assessed for matrix effects using different primary ion beams and sample composition. The presence of water in the cluster beam is associated with a reduction in the observed matrix effects, suggesting that chemically reactive ion beams may provide a route to more quantitative SIMS analysis of complex biological systems.

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Section: A Analysis Of Single-component Samplesmentioning

confidence: 90%

Matrix effects in biological SIMS using cluster ion beams of different chemical composition

2016

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Mass spectrometry imaging for plant biology: a review

et al. 2015

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Mass spectrometry imaging (MSI) is a developing technique to measure the spatio-temporal distribution of many biomolecules in tissues. Over the preceding decade, MSI has been adopted by plant biologists and applied in a broad range of areas, including primary metabolism, natural products, plant defense, plant responses to abiotic and biotic stress, plant lipids and the developing field of spatial metabolomics. This review covers recent advances in plant-based MSI, general aspects of instrumentation, analytical approaches, sample preparation and the current trends in respective plant research.

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Molecular ionization probability in cluster-SIMS

Wucher

2018

Journal of Vacuum Science &Amp; Technology B, Nanotechnology and Microelectronics: Materials, Processing, Measurement, and Phen

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The ionization efficiency of molecular species sputtered under bombardment with cluster ion beams is generally assumed to be rather low, but hard experimental evidence for this notion is still lacking. In order to estimate the prospects for possible sensitivity improvements in molecular secondary ion mass spectrometry (SIMS) experiments, the author therefore reports on recent experiments utilizing laser postionization (LPI) in order to determine an absolute ionization probability value for a few organic molecules sputtered under C60 cluster ion bombardment. The results show that the molecular ionization efficiency obtained under these bombarding conditions is of the order of 10−3, leaving at least 2 orders of magnitude for possible enhancement of the physical or a chemical ionization mechanism leading to the formation of (quasi-) molecular secondary ions. Analyzing the measured molecular LPI signal, the author demonstrates that about the same headroom exists for improvement of the currently achieved postionization efficiency. Using trehalose as a model system, the author then illustrates the level of improvement achieved by different attempts to utilize projectile induced surface chemistry in order to enhance the ionization efficiency. The results may be useful to guide new developments aimed at enhancing the detection sensitivity in molecular cluster-SIMS experiments.

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Comparing C₆₀⁺ and (H₂O)_n⁺ clusters for mouse brain tissue analysis

Cited by 4 publications

References 17 publications

Matrix effects in biological SIMS using cluster ion beams of different chemical composition

Matrix effects in biological SIMS using cluster ion beams of different chemical composition

Mass spectrometry imaging for plant biology: a review

Molecular ionization probability in cluster-SIMS

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