Comparing Anti-VEGF Antibodies and Aptamers on Paper Microfluidic-Based Platforms

Clayton, Katherine N.

doi:10.15368/theses.2012.83

Cited by 2 publications

(2 citation statements)

References 18 publications

(90 reference statements)

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“…The absorbent pad (AP) ( Figure 2 ) is generally composed of a cellulose fiber that can decrease the FT by hindering the backflow [ 73 , 74 , 75 ], absorbing and increasing the volume of the sample through the entire assay (LFIA) and reserves waste. In addition, the right AP selection can increase the performances of the test and the criteria for selection are the same as that of the sample pad (material, thickness, etc.)…”

Section: Lateral Flow Immunoassays (Lfia)mentioning

confidence: 99%

Monoclonal Antibodies Application in Lateral Flow Immunochromatographic Assays for Drugs of Abuse Detection

et al. 2021

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Lateral flow assays (lateral flow immunoassays and nucleic acid lateral flow assays) have experienced a great boom in a wide variety of early diagnostic and screening applications. As opposed to conventional examinations (High Performance Liquid Chromatography, Polymerase Chain Reaction, Gas chromatography-Mass Spectrometry, etc.), they obtain the results of a sample’s analysis within a short period. In resource-limited areas, these tests must be simple, reliable, and inexpensive. In this review, we outline the production process of antibodies against drugs of abuse (such as heroin, amphetamine, benzodiazepines, cannabis, etc.), used in lateral flow immunoassays as revelation or detection molecules, with a focus on the components, the principles, the formats, and the mechanisms of reaction of these assays. Further, we report the monoclonal antibody advantages over the polyclonal ones used against drugs of abuse. The perspective on aptamer use for lateral flow assay development was also discussed as a possible alternative to antibodies in view of improving the limit of detection, sensitivity, and specificity of lateral flow assays.

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Section: Lateral Flow Immunoassays (Lfia)mentioning

confidence: 99%

Monoclonal Antibodies Application in Lateral Flow Immunochromatographic Assays for Drugs of Abuse Detection

et al. 2021

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“…A channel characterization study was performed in order to determine optimal channel widths for routing fluid through cellulose membranes. In previous studies, the optimal channel width ranged from 1.6 -3.81 mm for backed nitrocellulose membrane [10,20]. Because this thesis used cellulose chromatography paper instead of 25 nitrocellulose membrane for the fluid routing layers, an ideal channel width for the cellulose membrane needed to be determined.…”

Section: Channel Characterization: Channel Widthsmentioning

confidence: 99%

FUNCTIONAL 3-D CELLULOSE & NITROCELLULOSE PAPER-BASED, MULITPLEX DIAGNOSTIC PLATFORMS WITHOUT COUPLING AGENTS

Tageson¹

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The purpose of this thesis was to demonstrate device functionality of 3-D paperbased, multiplex platforms, µPADs, without the use of coupling agents between layers. Previously, these platforms were fabricated with double-sided tape and cellulose powder to try to augment proper fluid routing, but difficulties with this method occurred. An acrylic housing unit with strategically placed pressure tabs was designed to aid horizontal and vertical fluid routing through the platform, thus eliminating the inconsistencies associated with coupling agents. Channel characterization studies, a COMSOL TM simulation, and development time studies were performed to aid device design and demonstrate device functionality. The implementation of this µPAD platform as a diagnostic instrument was validated via lateral flow immunoassays utilizing both biotinylated antibodies and biotinylated aptamers as capture reagents. Successful detection of the target analyte, IgE, as well as successful fluid routing through multiple layers of membrane was demonstrated by immunoassays performed on 3-D, multiplex platforms. Another important result determined the aptamers' ability to detect IgE to be statistically the same as the antibodies' ability; thus confirming aptamers as viable capture reagent alternatives to antibodies in lateral flow assays. Overall, this research project was performed to develop and validate via experiment a prototype paper-based microfluidic diagnostic device, µPAD, with the capability to detect multiple biomarkers on one platform.

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Comparing Anti-VEGF Antibodies and Aptamers on Paper Microfluidic-Based Platforms

Abstract: COMPARING ANTI-VEGF ANTIBODIES AND APTAMERS ON PAPER MICROFLUIDIC-BASED PLATFORMS

Cited by 2 publications

References 18 publications

Monoclonal Antibodies Application in Lateral Flow Immunochromatographic Assays for Drugs of Abuse Detection

Monoclonal Antibodies Application in Lateral Flow Immunochromatographic Assays for Drugs of Abuse Detection

FUNCTIONAL 3-D CELLULOSE & NITROCELLULOSE PAPER-BASED, MULITPLEX DIAGNOSTIC PLATFORMS WITHOUT COUPLING AGENTS

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