Comparative ultrastructural studies onBesnoitia besnoiti andBesnoitia caprae

Njenga, J. M.; Bwangamoi, O; Kang’ethe, Erastus K.; Mugera, G M; Mutiga, E.R.

doi:10.1007/bf01839312

Cited by 23 publications

(26 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Heydorn et al (1984) described the histological and ultrastructural appearance of Besnoitia cysts from the eyelid of goats in Kenya and showed that B. caprae bradyzoites had typical apicomplexa features, namely a three-layered pellicle, conoid, rhoptries, microtubules, micronemes, and polar ring. Similar features were reported for the related organisms, B. jellisoni (Sheffield 1966(Sheffield , 1968, B. besnoiti (Neuman 1974;Gobel et al 1985;Shkap et al 1988;Njenga et al 1995), and Toxoplasma gondii (Ogino and Yoneda 1966;Sheffield and Melton 1968;Aikawa et al 1977).…”

Section: Discussionsupporting

confidence: 75%

“…Micropore was formed by invagination of the outer membrane of the pellicle. This criterion is similar to that of B. caprae and B. besnoiti reported earlier (Njenga et al 1995). The outer cylindrical structure of the micropore was formed from thickening of the inner membrane of the pellicle and an outgrowth from the outer membrane of the pellicle at the point where invagination starts.…”

Section: Discussionsupporting

confidence: 72%

“…Although bovine besnoitiosis is reported from several parts of the world (Kharole et al 1979;Nobel et al 1981;Samish et al 1987;Lewis 1992;Njenga et al 1995), natural caprine besnoitiosis has only been reported from Iran (Cheema and Toofanian 1979;Bazargani et al 1987;Oryan and Sadeghi 1997) and Kenya (Bwangamoi et al 1989). It is stated that the species of Besnoitia found in goats in Iran and Kenya, is not infective for other animals (Bwangamoi et al 1989;Oryan and Sadeghi 1997).…”

Section: Introductionmentioning

confidence: 86%

“…The taxonomy of this genus also is poorly defined, and is based largely on the presence of characteristic bradyzoite-filled cysts in different species of intermediate hosts (Terrell and Stookey 1973;Wobeser 1976;Ayroud et al 1995;Oryan and Sadeghi 1997), and on the ultrastructural characteristics of the bradyzoites (van Heerden et al 1993;Njenga et al 1995;Dubey et al 2002;Dubey and Lindsay 2003). It is essential to have a better knowledge of the biology and life cycle of this parasite, including the possible definitive host(s), to formulate adequate treatment or control measures.…”

Section: Introductionmentioning

confidence: 98%

See 3 more Smart Citations

Ultrastructure and pathology of Besnoitia caprae in the naturally infected goats of Kerman, East of Iran

Oryan

Azizi

2008

Parasitol Res

View full text Add to dashboard Cite

A disease with clinical manifestations of thickening and alopecia of the skin over the lower limbs, around the eyes, face, and nose, thickening and shrinkage of the scrotum, and presence of white granular cysts in the sclero-conjunctiva in goats in Kerman Province, were reported to the Pathology Department of Shiraz Veterinary School. Primary histopathological studies demonstrated an outbreak of caprine besnoitiosis in this region. To study the histopathological and ultrastructural features of the disease, samples were collected from various organs of the suspected slaughtered goats for further investigations. In histopathological studies, dermis and subcutaneous fascia covering lower portion of the limbs, skin over frontal sinus, ear tips, scrotum, eye lids as well as the eye's sclera, epididymal and testicular parenchyma, and their tunics were severely infected with Besnoitia cysts. Tongue, pharynx, prepuce and penis, deeper striated muscles, subcutaneous bone matrices, abomasum, esophagus, subcutaneous tendons, and periosteal surfaces of the limb bones showed lower rates of infection. Except the vagina and vestibule, no cyst was observed in other female urogenital organs, the central nervous system, intestines, heart, liver, spleen, and different lymph nodes. The host reaction to the cysts was variable, ranging from the absence of inflammatory cells around intact normal cysts up to infiltration of macrophages, lymphocytes, plasma cells, eosinophils, fibroblasts, and connective tissues around the degenerated cysts. Ultrastructural studies showed this coccidian parasite belonged to eukaryotic protozoa, and the cystic form had the typical feature of the Besnoitia spp. of the apicomplexa. This study showed that the organism demonstrated ultrastructurally minor differences with other Besnoitia species infecting other animal species.

show abstract

Section: Discussionsupporting

confidence: 75%

Section: Discussionsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Ultrastructure and pathology of Besnoitia caprae in the naturally infected goats of Kerman, East of Iran

Oryan

Azizi

2008

Parasitol Res

View full text Add to dashboard Cite

show abstract

“…The histologic findings in the present study are consistent with those of the natural besnoitiosis. 3,6,27 There have been no previous morphometric studies on cysts and bradyzoites at histologic or ultrastructural levels, although Njenga et al 20 and Oryan and Azizi 24 described fine structural differences between Besnoitia isolated from goats and cattle.…”

Section: Discussionmentioning

confidence: 99%

Histopathologic and Ultrastructural Studies on Experimental Caprine Besnoitiosis

Oryan

Namazi

Silver³

2011

Vet Pathol

View full text Add to dashboard Cite

The distribution pattern and associated tissue reactions with progressive changes in Besnoitia caprae cysts were investigated in 6 experimentally infected 16-to 20-month-old male goats. Each goat was subcutaneously inoculated with approximately 13 Â 10 8 B caprae bradyzoites. The animals were examined daily for development of clinical besnoitiosis, and skin biopsies from distal parts of the limbs were taken at weekly intervals. At 15, 30, 60, 120, 180, and 365 days postinfection (DPI), 1 goat was euthanized. Samples were collected at autopsy from various organs for histologic and ultrastructural studies. No cysts were seen in tissue sections on 15, 30, and 365 DPI, but large numbers were present at 60, 120, and 180 DPI in the skin of the distal limbs, scrotum, and ears, with fewer in the tongue, palate, sclera, testicles, and spermatic cord. No cysts were seen in the lungs, liver, kidneys, spleen, central nervous system, or lymph nodes. Cyst numbers peaked at 60 DPI, then declined from 120 to 180 DPI. Degenerated cysts were relatively rare at 60 DPI but more numerous at 180 compared with 120. A granulomatous reaction-predominantly characterized by macrophages, lymphocytes, and plasma cells-surrounded each degenerated cyst. All goats showed testicular tubular degeneration with little or no spermatogenic activity. The sizes of cysts and their wall thickness, with the size of bradyzoites and some of their organelles, exhibited progressive chronologic changes.

show abstract

Viral and Protozoal Skin Diseases

Scott

2006

Color Atlas of Farm Animal Dermatology

View full text Add to dashboard Cite

Comparative ultrastructural studies onBesnoitia besnoiti andBesnoitia caprae

Cited by 23 publications

References 10 publications

Ultrastructure and pathology of Besnoitia caprae in the naturally infected goats of Kerman, East of Iran

Ultrastructure and pathology of Besnoitia caprae in the naturally infected goats of Kerman, East of Iran

Histopathologic and Ultrastructural Studies on Experimental Caprine Besnoitiosis

Viral and Protozoal Skin Diseases

Contact Info

Product

Resources

About