Comparative transcriptomic analysis of the multi-targeted effects of the herbal extracts against Escherichia coli O157:H7

Kim, Ki‐Suk; Lee, Jae-Youl; Kwon, Soo-Young; Yang, Hea Jung; Choi, Eun-Kyeong; Shin, Min Kyung; Ahn, Kwang Seok; Um, Jae Young; Lee, Jang-Hoon; Jang, Hyeung-Jin

doi:10.1007/s13206-012-6410-2

Cited by 6 publications

(8 citation statements)

References 40 publications

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“…Actually, B. falcatum is also commonly used in China and Korea [ 7 ]. In Japan, B. falcatum L is known for its therapeutic effects in the treatment of chronic hepatitis, auto-immune diseases and diabetes [ 8 – 11 ]. It is also used as an ingredient in herbal tea and traditional fermented beverages [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of chemical profiles between the root and aerial parts from three Bupleurum species based on a UHPLC-QTOF-MS metabolomics approach

Zhu

Liang

et al. 2017

BMC Complement Altern Med

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BackgroundBupleuri Radix (Chaihu) represents one of the most successful and widely used herbal medicines in Asia for the treatment of many diseases such as inflammatory disorders and infectious diseases over the past 2000 years. In the Chinese Pharmacopoeia, Chaihu is recorded as the dried roots of Bupleurum chinense DC. and B. scorzonerifolium Willd. (Umbelliferae). However, the widespread demand for the herb has tended to far outstrip the supply. Whether the aerial parts, which account for 70 ~ 85% of the dry weights of Bupleurum species, could be used as an alternative for the root has become an important scientific issue for the sustainable utilization of Bupleurum species. On the other hand, in some areas including the southeast of China as well as in Spain, the aerial parts of Bupleurum species have already been used in the folk medications. Therefore, to clarify whether the root and aerial parts of Bupleurum species are “equivalent” in the types and quantities of chemical constituents which subsequently influence their biological activities and therapeutic effects is of great importance for both the rational and sustainable use of this herb.MethodsIn the present study, the chemical profiles between the root and aerial parts of Bupleurum species from different species and collected from various locations were analyzed and compared by the ultra-high performance liquid chromatography quadrupole/time of flight-mass spectrometry (UHPLC-QTOF-MS).ResultsA total of 56 peaks were identified in the root and/or aerial parts from different batches of Bupleurum species, by comparison of references standards or with those reported in the literature. Principal Component Analysis (PCA) was conducted for displaying the differentiating clustering between these two parts.ConclusionThe results disclosed the distinct variations between them, which indicated that the aerial parts could not be used as an alternative of root from a chemodiversity perspective. The differentiating markers resulted from the PCA analysis could also be utilized for the differentiation between them. Further validation of their biological differences is anticipated in the future study.

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Section: Introductionmentioning

confidence: 99%

Comparison of chemical profiles between the root and aerial parts from three Bupleurum species based on a UHPLC-QTOF-MS metabolomics approach

Zhu

Liang

et al. 2017

BMC Complement Altern Med

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“…The proton-motive force is a result of the enhancement of the TCA cycle via an increase in the level of nicotinamide adenine dinucleotide. 52 The proton-motive force enhances antibiotic uptake. 53 Our results showing variations in metabolic pathways (including the TCA cycle) could be an antibiotic-resistance approach which might explain upregulation of the expression of some metabolic-pathway proteins in extracts having a lower effect on E. coli in contrast to extracts showing higher activity even though the expression of their proteins was downregulated.…”

Section: D-dige and Maldi Tof/tof Msmentioning

confidence: 99%

Antimicrobial Mechanism and Identification of the Proteins Mediated by Extracts from Asphaltum punjabianum and Myrtus communis

et al. 2020

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Myrtus communis (“myrtle”) and Asphaltum punjabianum (“shilajeet”) are a medicinal plant and a long-term-humified dead plant material, respectively. We studied their antibacterial and anticandidal activities against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Candida albicans. The activities of the aqueous extracts of the studied materials were measured using agar-well diffusion methods. Furthermore, proteomic analysis of treated microbial cells was conducted to identify affected proteins. The results showed both antibacterial and anticandidal activities for the myrtle extract (ME), while the shilajeet extract (SE) showed antibacterial activity only. The highest antimicrobial activity was observed against E. coli among the microbes tested; therefore, it was taken as the model for the proteomic analysis to identify the antimicrobial mechanism of ME and SE using two-dimensional electrophoresis. Upregulation of expression of 42 proteins and downregulation of expression of 6 proteins were observed in E. coli treated with ME, whereas 12 upregulated and 104 downregulated proteins were detected in E. coli treated with SE, in comparison with the control. About 85% of identified expressed proteins were from the cytoplasm and 15% from microbial cell walls, indicating the penetration of extracts inside cells. A higher percentage of expressed proteins was recorded for enzymatic activity. Our findings suggest that the major targets of the antibacterial action were proteins involved in the outer membrane, oxidative stress, and metabolism. Our data might reveal new targets for antimicrobial agents.

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“…High-valence silver nanoparticles are now considered as a possible substitute to antibiotics and appear to have a higher antimicrobial activity. It is also used to solve the problem of the emergence of bacterial multidrug resistance [16,17]. Recently, K. Yang et al synthesized the high-valence silver salt bis(hydrogen periodato)argentite(III) complex and investigated the wound healing and antibacterial efficacy against S. aureus and P. aeruginosa [18].…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization, and Antibacterial Activities of High-Valence Silver Propamidine Nanoparticles

et al. 2017

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Diabetic foot ulcer (DFU) is becoming more serious concern as it affects 95% of diabetic patients worldwide. It has been shown that the Staphylococcus aureus and other Gram-negative microorganisms are the main reasons behind this disease. Though many antibiotics are presently used to treat the DFU, due to increased bacterial resistance, new alternative therapies are always welcome. To address this alarming issue, we have designed and synthesized the high-valence silver propamidine (Ag(II)PRO) complex as well as nanoparticles and characterized both by usual spectroscopic methods. The reverse microemulsion technique has been applied to synthesize Ag(II)PRO nanoparticles and its antibacterial activity has been compared with zero-valence silver nanoparticles (AgNPs) with similar size. The antibacterial efficacies of Ag(II)PRO nanoparticles and AgNPs were tested against Gram-negative and Gram -positive organisms responsible for DFU. The newly synthesized high-valence Ag(II)PRO nanoparticles showed higher antibacterial activity compared to silver-only nanoparticles (AgNPs). This study concludes that the high-valence Ag(II)PRO nanoparticles show better antibacterial activity than AgNPs and they may serve as the next generation therapeutic agent for the diabetic wound care.

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Comparative transcriptomic analysis of the multi-targeted effects of the herbal extracts against Escherichia coli O157:H7

Cited by 6 publications

References 40 publications

Comparison of chemical profiles between the root and aerial parts from three Bupleurum species based on a UHPLC-QTOF-MS metabolomics approach

Comparison of chemical profiles between the root and aerial parts from three Bupleurum species based on a UHPLC-QTOF-MS metabolomics approach

Antimicrobial Mechanism and Identification of the Proteins Mediated by Extracts from Asphaltum punjabianum and Myrtus communis

Synthesis, Characterization, and Antibacterial Activities of High-Valence Silver Propamidine Nanoparticles

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