Comparative transcriptome analysis reveals a role for anthocyanin biosynthesis genes in the formation of purple peel in Minhou wild banana (<i>Musa itinerans Cheesman</i>)

Deng, Sufang; Cheng, Chunzhen; Liu, Zhuanxia; Chen, Yukun; Zhang, Zihao; Huang, Yuji; Lin, Yuling; Wang, Tianchi; Zhang, Lai

doi:10.1080/14620316.2018.1473055

Cited by 13 publications

(9 citation statements)

References 64 publications

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“…In preliminary studies, the variety ( Kitdamrongsont et al, 2008 ), content ( Javed et al, 2001 ; Pazmiño-Durán et al, 2001 ), and function ( Roobha et al, 2011 ) of banana anthocyanins have all been adequately determined, but the molecular mechanisms underlying anthocyanin biosynthesis and its regulation have only recently become recognized ( Deng et al, 2019 ). Additionally, the gene that is involved in anthocyanin biosynthesis in banana plants has not been previously characterized by genetic methods because of the low transgenic efficiency of bananas.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, it is difficult for us to determine whether the differentially expressed MYB is an activator or repressor solely through the relationship between gene expression and anthocyanin accumulation. Although Deng et al (2019) first identified many differentially expressed MYBs that correlate with anthocyanin biosynthesis in banana by transcriptomics, it is still difficult for us to determine whether differentially expressed MYB is an activator or a repressor.…”

Section: Introductionmentioning

confidence: 99%

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MaMYB4, an R2R3-MYB Repressor Transcription Factor, Negatively Regulates the Biosynthesis of Anthocyanin in Banana

et al. 2021

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Anthocyanins spatiotemporally accumulate in certain tissues of particular species in the banana plant, and MYB transcription factors (TFs) serve as their primary regulators. However, the precise regulatory mechanism in banana remains to be determined. Here, we report the identification and characterization of MaMYB4, an R2R3-MYB repressor TF, characterized by the presence of EAR (ethylene-responsive element binding factor–associated amphiphilic repression) and TLLLFR motifs. MaMYB4 expression was induced by the accumulation of anthocyanins. Transgenic banana plants overexpressing MaMYB4 displayed a significant reduction in anthocyanin compared to wild type. Consistent with the above results, metabolome results showed that there was a decrease in all three identified cyanidins and one delphinidin, the main anthocyanins that determine the color of banana leaves, whereas both transcriptome and reverse transcription–quantitative polymerase chain reaction analysis showed that many key anthocyanin synthesis structural genes and TF regulators were downregulated in MaMYB4 overexpressors. Furthermore, dual-luciferase assays showed that MaMYB4 was able to bind to the CHS, ANS, DFR, and bHLH promoters, leading to inhibition of their expression. Yeast two-hybrid analysis verified that MaMYB4 did not interact with bHLH, which ruled out the possibility that MaMYB4 could be incorporated into the MYB-bHLH-WD40 complex. Our results indicated that MaMYB4 acts as a repressor of anthocyanin biosynthesis in banana, likely due to a two-level repression mechanism that consists of reduced expression of anthocyanin synthesis structural genes and the parallel downregulation of bHLH to interfere with the proper assembly of the MYB-bHLH-WD40 activation complex. To the best of our knowledge, this is the first MYB TF that regulates anthocyanin synthesis that was identified by genetic methods in bananas, which will be helpful for manipulating anthocyanin coloration in banana programs in the future.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MaMYB4, an R2R3-MYB Repressor Transcription Factor, Negatively Regulates the Biosynthesis of Anthocyanin in Banana

et al. 2021

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“…Although most studies have identified lncRNAs in anthocyanins-producing plants, among which only Yuanxiu Lin et al found that lncRNAs in strawberry may regulate anthocyanins synthesis by competitively binding miRNA [50]. The others have not linked lncRNAs to target genes involved in the anthocyanins synthesis pathway [51][52][53]. We predicted three lncRNAs involved in the regulation of anthocyanins-related genes, as one of the other possible mechanisms for the production of anthocyanins in fungi, which providing a possible direction for exploring the mechanism of anthocyanins production.…”

Section: Discussionmentioning

confidence: 99%

Identification of novel anthocyanins synthesis pathway from fungus, Aspergillus sydowii H-1

Zhang

Zeng

et al. 2019

Preprint

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Background Anthocyanins are common substance in people's production and life. Usually it was used as a natural dye, more and more studies have shown that anthocyanins have potential preventive and / or therapeutic effects on human health, such as improving cardiovascular function and treating obesity. However, anthocyanins are generally thought to occur only in natural plants. The difficulty of mass production limits the application of anthocyanins in industry. Production of anthocyanins from microbial fermentation is not restricted by time, space and environmental conditions as compared with plants, which makes it more anxious to obtain anthocyanins from microorganisms. Here, we firstly reported a fungus, Aspergillus sydowii H-1which can produce anthocyanins under the specific fermentation, and analyzed its metabolome and transcriptome. Results All of the 31 gene loci related to anthocyanins synthesis pathway were identified in A. sydowii H-1, and almost all of those genes display high correlation with the data from metabolome. Among them, chalcone synthase gene (CHS) and cinnamate-4-hydroxylase gene (C4H) were only found using denovo assembly without reference, and interestingly, the best hits of these two genes all belong to plant species. Therefore, the plant-derived genes, CHS and C4H, may be one of the reasons why A. sydowii H-1 can produce anthocyanins. We also identified 530 lncRNAs in our datasets, and among them, three lncRNAs targeted to the genes related to anthocyanins biosynthesis via cis-regulation, which likewise provide clues for the underlying mechanism of anthocyanins’ production in the fungi. Conclusions In this study, we discovered a fungus, Aspergillus sydowii H-1, which can produce anthocyanins under the specific fermentation condition. It’s the first report that anthocyanins can be produced in non-plant species.

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“…In banana, the RNA sequencing of epidermal cells of the purple and green parts of peel suggested that anthocyanin gene expression and, subsequently, anthocyanin metabolism contributed to the formation of the purple peel of “Minhou”, a wild banana [ 128 ]. Carotenoids accumulated during banana ripening due to the high expression of genes such as LCYβ (Lycopene β cyclase) and GGPS (Geranylgeranyl diphosphate synthase), especially MaLCYB1.1 and MaLCYB1.2 , regulated by ripening inducible transcription factor, MaSPL16 and low expression of CCD s (Carotenoid cleavage dioxygenase 1) and NCED s (9-cis epoxy carotenoid dioxygenase) in ripe banana [ 30 , 46 ].…”

Section: Fruit Ripeningmentioning

confidence: 99%

Genomic Approaches for Improvement of Tropical Fruits: Fruit Quality, Shelf Life and Nutrient Content

Mathiazhagan

Chidambara

Laxman

et al. 2021

Genes

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The breeding of tropical fruit trees for improving fruit traits is complicated, due to the long juvenile phase, generation cycle, parthenocarpy, polyploidy, polyembryony, heterozygosity and biotic and abiotic factors, as well as a lack of good genomic resources. Many molecular techniques have recently evolved to assist and hasten conventional breeding efforts. Molecular markers linked to fruit development and fruit quality traits such as fruit shape, size, texture, aroma, peel and pulp colour were identified in tropical fruit crops, facilitating Marker-assisted breeding (MAB). An increase in the availability of genome sequences of tropical fruits further aided in the discovery of SNP variants/Indels, QTLs and genes that can ascertain the genetic determinants of fruit characters. Through multi-omics approaches such as genomics, transcriptomics, metabolomics and proteomics, the identification and quantification of transcripts, including non-coding RNAs, involved in sugar metabolism, fruit development and ripening, shelf life, and the biotic and abiotic stress that impacts fruit quality were made possible. Utilizing genomic assisted breeding methods such as genome wide association (GWAS), genomic selection (GS) and genetic modifications using CRISPR/Cas9 and transgenics has paved the way to studying gene function and developing cultivars with desirable fruit traits by overcoming long breeding cycles. Such comprehensive multi-omics approaches related to fruit characters in tropical fruits and their applications in breeding strategies and crop improvement are reviewed, discussed and presented here.

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Comparative transcriptome analysis reveals a role for anthocyanin biosynthesis genes in the formation of purple peel in Minhou wild banana (Musa itinerans Cheesman)

Cited by 13 publications

References 64 publications

MaMYB4, an R2R3-MYB Repressor Transcription Factor, Negatively Regulates the Biosynthesis of Anthocyanin in Banana

MaMYB4, an R2R3-MYB Repressor Transcription Factor, Negatively Regulates the Biosynthesis of Anthocyanin in Banana

Identification of novel anthocyanins synthesis pathway from fungus, Aspergillus sydowii H-1

Genomic Approaches for Improvement of Tropical Fruits: Fruit Quality, Shelf Life and Nutrient Content

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