Comparative Transcriptome Analysis Revealing the Potential Mechanism of Low-Temperature Stress in Machilus microcarpa

Abstract

Machilus microcarpa is a rare national tree species in China and possesses important ornamental and ecological value. M. microcarpa can be planted in low-temperature areas, depending on whether its seedlings can withstand the harm. To face this problem, the annual seedlings of M. microcarpa were subjected to five temperature treatments, and eight physiological indicators were measured. Furthermore, comparative transcriptome analysis was performed between M. microcarpa leaves treated at 25°C and −2.8°C. A total of 9,385 differentially expressed genes (DEGs) were involved in low-temperature stress in M. microcarpa. An upregulated (cobA) and five downregulated (HEM, CHLM, CRD, CLH, and PORA) genes associated with the porphyrin and chlorophyll metabolism pathway may reduce chlorophyll synthesis under low-temperature stress. Upregulation of six DEGs (two GAPDHs, PFK, PGAM, PDC, and PK) involved in the glycolysis/gluconeogenesis pathway provided energy for M. microcarpa under adverse cold conditions. Thirteen upregulated and seven downregulated genes related to antioxidant enzymes were also observed under low-temperature stress. Candidate transcription factors (TFs) played key roles in signal transduction under low-temperature stress in M. microcarpa, and quantitative real-time PCR (qRT-PCR) analysis validated the RNA-seq data. The results provide valuable information for further studies on the cold response mechanisms for low-temperature stress in M. microcarpa.

Global crotonylatome and GWAS revealed a TaSRT1 - TaPGK model regulating wheat cold tolerance through mediating pyruvate

“…Glycolysis is intimately related with cold tress ( 28 – 30 ), and PGK as a key enzyme plays crucial roles in glycolysis ( 43 ). Previous studies indicated that glycolytic genes (e.g., GAPC , PFK , PGM , ENO , and PK ) could be induced by cold stress ( 28 – 30 ). In this study, we found that TaPGK up-regulated the expression of TaGAPC , TaPGM , TaENO , and TaPK.…”

“…PGK is a highly conserved reversible enzyme that catalyzes the conversion of 1,3-bisphosphoglycerate and adenosine diphosphate to 3-phosphoglycerate and adenosine triphosphate in glycolysis and mediates the reverse reaction in gluconeogenesis and the Calvin-Benson cycle (43). Glycolysis is intimately related with cold tress (28)(29)(30), and PGK as a key enzyme plays crucial roles in glycolysis (43). Previous studies indicated that glycolytic genes (e.g., GAPC, PFK, PGM, ENO, and PK) could be induced by cold stress (28)(29)(30).…”

“…In plants, PGK is a highly conserved reversible key enzyme in glycolysis (43). Glycolysis plays an important role in plant cold response (28)(29)(30). To explain why TaPGK affects wheat cold tolerance, we sequenced the transcriptome of TaPGK-overexpressed plants and WT before and after the cold stress.…”

“…Some crucial glycolytic genes are directly related to cold stress. In Machilus macrocarpa , the up-regulation of glycolytic genes, including glyceraldehyde 3-phosphate dehydrogenase ( GAPC ), phosphofructokinase ( PFK ), phosphoglycerate mutase ( PGM ), and pyruvate kinase ( PK ), could provide energy to enhance cold stress ( 28 ). In orange, glycolytic genes fructose-bisphosphate aldolase ( FBA ) and enolase ( ENO ) were strongly induced by cold stress ( 29 ).…”

Multi-Omics Analysis of Vicia cracca Responses to Chronic Radiation Exposure in the Chernobyl Exclusion Zone

“…Changes in the antenna complex LHCII and photosystems I and II ( Figure 10 ) were observed, which suggests that the photosynthetic machinery may undergo alterations in response to chronic radiation exposure. Downregulated DEGs included ISPE_SOLLC , PUR5_VIGUN , PGL1B_ARATH , FTSI1_ARATH , CHLM_ARATH , and PGKH_WHEAT ( Table 4 ), which are associated with chloroplast development and chlorophyll biosynthesis [ 41 , 42 , 43 , 44 , 45 ]. Decreased expression can be either associated with redox damage of the ETC or developmental changes under chronic irradiation conditions.…”