Comparative transcriptome analysis of pepper (Capsicum annuum) revealed common regulons in multiple stress conditions and hormone treatments

Abstract

Global transcriptome analysis revealed common regulons for biotic/abiotic stresses, and some of these regulons encoding signaling components in both stresses were newly identified in this study. In this study, we aimed to identify plant responses to multiple stress conditions and discover the common regulons activated under a variety of stress conditions. Global transcriptome analysis revealed that salicylic acid (SA) may affect the activation of abiotic stress-responsive genes in pepper. Our data indicate that methyl jasmonate (MeJA) and ethylene (ET)-responsive genes were primarily activated by biotic stress, while abscisic acid (ABA)-responsive genes were activated under both types of stresses. We also identified differentially expressed gene (DEG) responses to specific stress conditions. Biotic stress induces more DEGs than those induced by abiotic and hormone applications. The clustering analysis using DEGs indicates that there are common regulons for biotic or abiotic stress conditions. Although SA and MeJA have an antagonistic effect on gene expression levels, SA and MeJA show a largely common regulation as compared to the regulation at the DEG expression level induced by other hormones. We also monitored the expression profiles of DEG encoding signaling components. Twenty-two percent of these were commonly expressed in both stress conditions. The importance of this study is that several genes commonly regulated by both stress conditions may have future applications for creating broadly stress-tolerant pepper plants. This study revealed that there are complex regulons in pepper plant to both biotic and abiotic stress conditions.

Dynamics of the chili pepper transcriptome during fruit development

“…More recently, a global transcriptomic analysis of chili pepper plants treated with different biotic/abiotic stresses was carried out to investigate the participation of signaling components (regulons) in both types of stresses. This study targeted the involvement of salicylic acid in the activation of abiotic stress-responsive genes, methyl jasmonate and ethylene in regulating biotic stress-responsive genes, and abscisic acid in regulating both biotic and abiotic stress-responsive genes [20]. …”

Pepper Crop under Climate Change: Grafting as an Environmental Friendly Strategy

“…Some other abiotic stresses include: low temperature because it affects pepper vegetative development and reproduction as it disturbs how flower female organs function, and the amount of viable pollen grains per flower [43,44]; high temperature and radiation promote stunted growth, a lower photosynthetic rate, increased respiration, and poor water and ion uptake [24,26]. Therefore, using different shading screens is considered an alternative to overcome these problems [45,46].…”

Jasmonic acid causes short- and long-term alterations to the transcriptome and the expression of defense genes in sugarbeet roots

“…KEGG pathway analysis similarly indicated a preponderance of JA-altered unigenes involved in metabolism, especially secondary metabolism, as a large number of unigenes mapped to metabolic pathways, a category that includes enzymes involved in primary and secondary metabolism, and to the biosynthesis of secondary metabolites pathway. Large changes in the transcriptome due to exogenous JA or MeJA treatment have been previously reported for a number of plant species (Babst et al, 2009;Gális et al, 2006;Hao et al, 2015;Lee and Choi, 2013;Oliveira et al, 2015;Ramírez-Estrada et al, 2016;Salzman et al, 2005;Sasaki-Sekimoto et al, 2005;Sun et al, 2013b;Zang et al, 2015). As in sugarbeet root, cellular and molecular processes, and binding and catalytic functions typically dominate GO classifications, and metabolic pathways and biosynthesis of secondary metabolites generally predominate as the KEGG pathways to which JA-altered genes map (Oliveira et al, 2015;Zang et al, 2015).…”