Comparative transcriptome analysis of gene expression patterns on B16F10 melanoma cells under Photobiomodulation of different light modes

Chen, Zeqing; Qin, Haiming; Lin, Shangfei; Lu, Zhanbin; Fan, Xuewei; Liu, Xuwen; Liu, Muqing

doi:10.1016/j.jphotobiol.2021.112127

Cited by 13 publications

(6 citation statements)

References 37 publications

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“…Figure 5 shows the results under the condition of light intensity (5 mW/cm 2 ). As the light dose increased, the temperature in the medium increased slightly and remained at about 37.4 • C ± 0.4 • C, the temperature changed within 1 • C. Our previous studies have demonstrated that the effect of light on cells is not caused by changing the temperature of the culture medium [19]. This result also demonstrated that the inhibitory effect of PBM treatment on the inflammation of human gingival fibroblasts was independent of changing temperature.…”

Section: Temperature Changedmentioning

confidence: 62%

“…Periodontitis experiments in rats have also demonstrated that 660 nm PBM could reduce inflammation and accelerate wound healing [18]. Experiments on HGFs in vitro also showed that 635 nm PBM could reduce the release of PGE2 and achieve an anti-inflammatory effect [19]. However, these studies did not explore different doses of PBM on periodontitis.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of 630 nm Photobiomodulation on the Anti-Inflammatory Effect of Human Gingival Fibroblasts

et al. 2021

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Periodontal disease is the most common oral chronic inflammatory disease in humans. Recent studies have indicated that red light Photobiomodulation (PBM) could inhibit cell inflammation effectively, but the effect of different doses of PBM on the treatment of inflammation has to be improved. Thus, this study was aimed to investigate the effects of various doses of PBM (630 ± 30 nm, (1) 5 mW/cm2, 1 J/cm2, 200 s; (2) 5 mW/cm2, 3 J/cm2, 600 s; (3) 5 mW/cm2, 9 J/cm2, 1800 s; (4) 5 mW/cm2, 18 J/cm2, 3600 s; (5) 5 mW/cm2,36 J/cm2, 7200 s) on the anti-inflammatory response of human gingival fibroblasts. Our results suggested that PBM (630 ± 30 nm) with doses of 18 J/cm2 and 36 J/cm2 could significantly inhibit the production of inflammatory cytokines such as Prostaglandin E2 (PGE2) and IL-8, presumably due to the fact that a high dose of PBM treatment could reduce intracellular Reactive oxygen species (ROS) in human gingival fibroblasts, thus reducing the expression of COX-2 enzyme. In addition, it was found that treatment with different doses of PBM (630 ± 30 nm) did not result in reduced mitochondrial membrane potential and mitochondrial dysfunction in human gingival fibroblasts. Our study provides a theoretical reference for the selection of PBM parameters and the application of PBM in the clinical treatment of periodontitis.

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Section: Temperature Changedmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

The Effect of 630 nm Photobiomodulation on the Anti-Inflammatory Effect of Human Gingival Fibroblasts

et al. 2021

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“…Blue light, as a visible light with a wavelength range of 400–500 nm, has been clinically applied successfully comprising neonatal jaundice, psoriasis (Pv), atopic dermatitis (AD), eczema, acne, and other inflammatory skin conditions, although the number of available clinical studies evaluating the efficacy of blue light treatment is still limited ( 103 , 136 ). Furthermore, recent studies have demonstrated that blue light irradiation inhibits the proliferation of multiple types of cancer cells in vitro and in vivo , including colon cancer cells ( 14 , 144 , 145 ), malignant glioma cells ( 12 ), melanoma cells ( 10 , 146 , 147 ), B-cell lymphoma cells ( 13 ), fibrosarcoma cells ( 14 ), pancreatic cancer cells ( 15 ), cutaneous squamous cell carcinoma cells (CSCC), epidermoid carcinoma cells ( 148 , 149 ), leukemia cells (Kasumi-1) ( 150 ), bladder cancer cells ( 151 ), colorectal cancer cells ( 152 , 153 ), breast cancer cells ( 91 ), and human OS cells ( 95 , 98 ). Here, we have briefly summarized the relevant research of blue light PBMT in the field of cancer treatment ( Table 1 ).…”

Section: Blue Light Photobiomodulation Therapy In Cancer Treatmentmentioning

confidence: 99%

Progress of phototherapy for osteosarcoma and application prospect of blue light photobiomodulation therapy

Yang

Jiang

et al. 2022

Front. Oncol.

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Osteosarcoma (OS) is the most common primary malignant bone tumor that mainly affects the pediatric and adolescent population; limb salvage treatment has become one of the most concerned and expected outcomes of OS patients recently. Phototherapy (PT), as a novel, non-invasive, and efficient antitumor therapeutic approach including photodynamic therapy (PDT), photothermal therapy (PTT), and photobiomodulation therapy (PBMT), has been widely applied in superficial skin tumor research and clinical treatment. OS is the typical deep tumor, and its phototherapy research faces great limitations and challenges. Surprisingly, pulse mode LED light can effectively improve tissue penetration and reduce skin damage caused by high light intensity and has great application potential in deep tumor research. In this review, we discussed the research progress and related molecular mechanisms of phototherapy in the treatment of OS, mainly summarized the status quo of blue light PBMT in the scientific research and clinical applications of tumor treatment, and outlooked the application prospect of pulsed blue LED light in the treatment of OS, so as to further improve clinical survival rate and prognosis of OS treatment and explore corresponding cellular mechanisms.

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“…Most of the current studies on photobiomodulation for melanoma treatment are in vitro studies. Liu et al treated melanoma with different light modes of blue light separately and found that different light modes led to different gene expression levels in melanoma by RNA‐Seq 86 . PBM can not only regulate the signal pathway and inhibit the proliferation of melanoma by controlling the protein‐protein interaction in cells, but also treat melanoma by reacting with photosensitizers.…”

Section: Future Studiesmentioning

confidence: 99%

The review of the light parameters and mechanisms of Photobiomodulation on melanoma cells

Chen

Huang

Liu

2021

Photoderm Photoimm Photomed

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Photobiomodulation (PBM) uses low‐intensity visible or near‐infrared light to produce beneficial effects on cells or tissues, such as brain therapy, wound healing. Still there is no consistent recommendation on the parameters (dose, light mode, wavelength, irradiance) and protocols (repetition, treatment duration) for its clinical application. Herein, we summarize the current PBM parameters for the treatment of melanoma, and we also discuss the potential photoreceptors and downstream signaling mechanisms in the PBM treatment of melanoma cells. It is hypothesized that PBM may inhibit the melanoma cells by activating mitochondria, OPNs, and other receptors. Regardless of the underlying mechanisms, PBM has been shown to be beneficial in treating melanoma. Through further in‐depth studies of the underlying potential mechanisms, it can strengthen the applications of PBM for the therapy of melanoma.

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Comparative transcriptome analysis of gene expression patterns on B16F10 melanoma cells under Photobiomodulation of different light modes

Cited by 13 publications

References 37 publications

The Effect of 630 nm Photobiomodulation on the Anti-Inflammatory Effect of Human Gingival Fibroblasts

The Effect of 630 nm Photobiomodulation on the Anti-Inflammatory Effect of Human Gingival Fibroblasts

Progress of phototherapy for osteosarcoma and application prospect of blue light photobiomodulation therapy

The review of the light parameters and mechanisms of Photobiomodulation on melanoma cells

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