Comparative transcript profiling of the fertile and sterile flower buds of pol CMS in B. napus

Abstract

BackgroundThe Polima (pol) system of cytoplasmic male sterility (CMS) and its fertility restoration gene Rfp have been used in hybrid breeding in Brassica napus, which has greatly improved the yield of rapeseed. However, the mechanism of the male sterility transition in pol CMS remains to be determined.ResultsTo investigate the transcriptome during the male sterility transition in pol CMS, a near-isogenic line (NIL) of pol CMS was constructed. The phenotypic features and sterility stage were confirmed by anatomical analysis. Subsequently, we compared the genomic expression profiles of fertile and sterile young flower buds by RNA-Seq. A total of 105,481,136 sequences were successfully obtained. These reads were assembled into 112,770 unigenes, which composed the transcriptome of the bud. Among these unigenes, 72,408 (64.21%) were annotated using public protein databases and classified into functional clusters. In addition, we investigated the changes in expression of the fertile and sterile buds; the RNA-seq data showed 1,148 unigenes had significantly different expression and they were mainly distributed in metabolic and protein synthesis pathways. Additionally, some unigenes controlling anther development were dramatically down-regulated in sterile buds.ConclusionsThese results suggested that an energy deficiency caused by orf224/atp6 may inhibit a series of genes that regulate pollen development through nuclear-mitochondrial interaction. This results in the sterility of pol CMS by leading to the failure of sporogenous cell differentiation. This study may provide assistance for detailed molecular analysis and a better understanding of pol CMS in B. napus.

Integrative RNA- and miRNA-Profile Analysis Reveals a Likely Role of BR and Auxin Signaling in Branch Angle Regulation of B. napus

“…After checking the RNA quality, cDNA libraries were constructed and used for Illumina sequencing following previous methods [64]. Raw data was filtered by removing adapters and low quality data and the resulting clean data was aligned to the Brassica napus reference genome (available at: ).…”

Transcriptome profiling of differentially expressed genes in cytoplasmic male-sterile line and its fertility restorer line in pigeon pea (Cajanus cajan L.)

“…Transcriptome sequencing offers ease of identification and evaluation of thousands of genes in a single analysis [53]. Transcriptome analysis of the cytoplasmic male-sterile lines has been previously reported in many crops such as radish [54], watermelon [55], soybean [56], tomato [57], Brassica napus [58], chili pepper [59],cotton [60][61][62], sweet orange [63] leading to identification of several candidate genes in these systems. In this study, comparative transcriptome analysis of an A 2 CMS system derived cytoplasmic male-sterile, and its fertility restorer line in pigeon pea was performed primarily to detect differentially expressed genes participating in pollen development which might help in understanding the CMS mechanism in this crop.…”

Comparative Transcriptome Analysis Reveals the Potential Mechanism of Abortion in Tobacco sua-Cytoplasmic Male Sterility

“…In the present study, four of these five mitochondrial encoded genes were downregulated in msZY, and there was lower ATP content in young flower buds of msZY than in ZY (Figure 4). Abnormal energy metabolism or DEGs enriched in energy metabolism were observed in pol CMS [7], SaNa-1A CMS [59], and hau CMS [15]. Decreased ATP production was considered the reason for pollen abortion in transgenic tobacco [27] and rice [60].…”

“…In stages 9−14, the microspores develop into mature pollen grains. In most types of CMS, abortion mainly occurs during microsporogenesis (stages 4−8) [6,7] and pollen maturation (stages 9−14) [8][9][10][11]. The molecular mechanism of CMS in these stages has been studied in detail [12].…”