Comparative Transcriptome Analysis Reveals the Potential Mechanism of Abortion in Tobacco sua-Cytoplasmic Male Sterility

Abstract

sua-CMS (cytoplasmic male sterility) is the only male sterile system in tobacco breeding, but the mechanism of abortion is unclear. Cytological characteristics show that abortion in the sua-CMS line msZY occurs before the differentiation of sporogenous cells. In this study, a comparative transcriptomic analysis was conducted on flower buds at the abortion stage of msZY and its male fertile control ZY. A total of 462 differentially expressed genes were identified in msZY and ZY, which were enriched via protein processing in the endoplasmic reticulum (ER), oxidative phosphorylation, photosynthesis, and circadian rhythm-plant by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. Most genes were downregulated in the ER stress pathway, heat-shock protein family, F1F0-ATPase encoding by the mitochondrial genome, and differentiation of stamens. Genes in the programmed cell death (PCD) pathway were upregulated in msZY. The transcriptome results were consistent with those of qRT-PCR. Ultrastructural and physiological analyses indicted active vacuole PCD and low ATP content in msZY young flower buds. We speculated that PCD and a deficiency in ATP synthesis are essential for the abortion of sua-CMS. This study reveals the potential mechanism of abortion of tobacco sua-CMS.

Whole-transcriptome analysis and construction of an anther development-related ceRNA network in Chinese cabbage (Brassica campestris L. ssp. pekinensis)

“…1 c). KEGG enrichment analysis revealed that the targets of DEmiRNAs were significantly enriched in several pathways that may be related to anther development, including ‘plant hormone signal transduction’, which plays an important regulatory role in plant anther development 31 , ‘endocytosis’, which is essential for the development of male reproductive organs in Arabidopsis 32 , ‘plant–pathogen interaction’, which plays a dominate role in recessive genic male sterility of cabbage 33 , ‘protein processing in the endoplasmic reticulum’, and ‘oxidative phosphorylation’, which were also significantly enriched in the comparative transcriptome analysis of tobacco sua -cytoplasmic male sterility, indicating that they may be involved in anther development 34 (Fig. 1 d).…”

Comparative Transcriptome Analysis Reveals a Potential Regulatory Network for Ogura Cytoplasmic Male Sterility in Cabbage (Brassica oleracea L.)

“…Tobacco sua -CMS is the only CMS system in tobacco breeding. A comparative transcriptomic analysis showed that genes involved in the ER stress pathway, heat shock protein family, F 1 F 0 -ATPase, and differentiation of stages are down-regulated in sua -CMS, while genes in the programmed cell death (PCD) pathway are significantly up-regulated, indicating that PCD and defects in ATP synthesis are crucial to the pollen abortion of sua -CMS [ 36 ]. A proteomic analysis of Shaan2A-CMS in B. napus showed that the differentially expressed proteins were related mainly to carbohydrate metabolism, energy metabolism, and genetic information processing pathways [ 37 ].…”