Comparative study with monospecific and monoclonal antibodies gainst a 65 K human cytomegalovirus protein

Abstract

Immunofluorescence assay using monospecific and monoclonal antibodies to the 65 K major protein of human cytomegalovirus (HCMV) was carried out to monitor the expression of this protein in infected cells. Regardless of differences in the reactivity of the monoclonal antibodies, as determined by immunoblotting and immunofluorescent staining, all stained cytoplasmic inclusion bodies localized to the site of the HCMV-induced receptor for the Fc portion of IgG, suggesting that most of the 65 K major protein of HCMV colocalizes with the HCMV-induced FcR.

Human cytomegalovirus structural proteins

“…Recently, Riddell and coworkers (unpublished results), have shown that the majority of CD8 +, human leukocyte antigen (HLA) class I-restricted cytotoxic T cell clones generated by repeated stimulation of PBMC from immune donors with autologous HCMV-infected fibroblasts, reacted with ppUL83. Britt & Auger, 1985;Chardonnet et al, 1983;Gerna et al, 1992;Grefte et al, 1992;Kim et al, 1983;Lucas et al, 1988;Michelson et al, 1984Michelson et al, , 1985Pereira et al, 1982a;Plachter et al, 1990;Redmond et al, 1986;Revello et al, 1992;Rodgers et al, 1985;Shuster et al, 1985;van der Bij et al, 1988b) as well as human MAbs (Hoshi et al, 1987;Foung et al, 1989;Kitamura et al, 1990;Ohlin et al, 1991;Sutherland et al, 1987;Shimokawa et al, 1988) to this protein have been described (see Table 1). A murine MAb to ppUL82 was described by Nowak et al (1984b), but the hybridoma is no longer available.…”

Human monoclonal antibodies to human cytomegalovirus derived from peripheral blood lymphocytes of healthy adults

“…HMAbs A-2 and A-3 chiefly stained the nucleus with a sharply defined granular pattern at 48 hr after infection and stained the cytoplasm along with a weak diffuse nuclear staining at 5 days. This observation suggested that the antigens identified were processed while the virions were being assembled in the infected cells [Shimokawa et al, 1988;Weiner et al, 19851. A-1 showed a staining pattern similar to A-2 and A-3 when IFA was performed using a clinical isolate of HCMV, suggesting that there may be some differences in antigen expression between different strains of the virus. Immunoblotting experiments also divided these four HMAbs into two groups.…”