Human monoclonal antibodies to human cytomegalovirus derived from peripheral blood lymphocytes of healthy adults

Abstract

Immunoglobulin G class human monoclonal antibodies to human cytomegalovirus (HCMV) were produced by the fusion of Epstein-Barr virus-transformed B cells and a murine myeloma cell line. The B cells were derived from the peripheral blood lymphocytes of healthy adult volunteers. Four hybridomas producing HCMV-specific monoclonal antibodies were established and each of four antibodies immunoprecipitated an HCMV-specific protein with a molecular weight of 68 kDa. However, the antibodies differed in some of their properties as characterized by indirect immunofluorescence assay and immunoblotting studies, due to the detection of different epitopes on the reacting antigen. None of the four antibodies had any virus neutralizing activity.

Human cytomegalovirus structural proteins

“…Recently, Riddell and coworkers (unpublished results), have shown that the majority of CD8 +, human leukocyte antigen (HLA) class I-restricted cytotoxic T cell clones generated by repeated stimulation of PBMC from immune donors with autologous HCMV-infected fibroblasts, reacted with ppUL83. Britt & Auger, 1985;Chardonnet et al, 1983;Gerna et al, 1992;Grefte et al, 1992;Kim et al, 1983;Lucas et al, 1988;Michelson et al, 1984Michelson et al, , 1985Pereira et al, 1982a;Plachter et al, 1990;Redmond et al, 1986;Revello et al, 1992;Rodgers et al, 1985;Shuster et al, 1985;van der Bij et al, 1988b) as well as human MAbs (Hoshi et al, 1987;Foung et al, 1989;Kitamura et al, 1990;Ohlin et al, 1991;Sutherland et al, 1987;Shimokawa et al, 1988) to this protein have been described (see Table 1). A murine MAb to ppUL82 was described by Nowak et al (1984b), but the hybridoma is no longer available.…”

Exploring the Native Human Antibody Repertoire to Create Antiviral Therapeutics

“…Many native human monoclonal antibodies specific for CMV have been described (Emanuel et al 1984;Redmond et al 1986;Foung et al 1989;Bron et al 1990;Kitamura et al 1990;Drobyski et al 1991;Gustafsson et al 1991;Ohizumi et al 1992;Ohlin et al 1993;Rioux et al 1994). Some of these were found to be capable of neutralizing CMV in vitro (Redmond et al 1986;Foung et al 1989;Ohizumi et al 1992;Ohlin et al 1993).…”