Comparative study on laccase activity of white rot fungi under submerged fermentation with different lignocellulosic wastes

Abstract

Different Pleurotus ostreatus and Flammulina velutipes species were compared relative to their ability to produce laccase in submerged fermentation of various lignocellulosic wastes. Fungi cultivation in identical culture conditions revealed wide differences among both species and strains of the same species. The laccase secretion ability of P. ostreatus strains was superior to F. velutipes strains. Maximum laccase production on cottonseed hull, corncob, and poplar wood was secreted by P. ostreatus CY 568, P. ostreatus CCEF 89, and P. ostreatus CY 568, respectively. The nature of lignocellulosic materials played an important role in determining the expression of laccase potential of fungi. The presence of cottonseed hull improved laccase activity and accelerated the rate of enzyme production. Maximum laccase production on cottonseed hull was nearly 1.29-fold and 1.53-fold higher than that on corncob and poplar wood, respectively. Laccase activity was detected in almost all tested strains on cottonseed hull on the first day, while only a few strains on poplar wood and corncob were detected on the first day. These findings will be helpful for selecting the appropriate strain in industrial applications and for optimization of integrated industrial laccase production.

Evaluation of Laccase Activities by Three Newly Isolated Fungal Species in Submerged Fermentation With Single or Mixed Lignocellulosic Wastes

“…The optimal conditions for laccase production from T. versicolor were found at 35 • C and 5 g/L of wheat bran as substrate, reaching approximately 200 U/ml on 11 days in submerged fermentation (Atilano-Camino et al, 2020). An et al (2020b) reported that laccase production from P. ostreatus and Flammulina velutipes strains grown on cottonseed hull was better than that on corncob or poplar wood, and laccase production from P. ostreatus CCEF 89 grown on cottonseed hull, corncob, and poplar sawdust ranged from 61.38 ± 4.09 to 748.24 ± 9.53 U/L, 26.12 ± 2.28 to 699.12 ± 44.91 U/L, and 3.32 ± 0.30 to 509.75 ± 15.43 U/L, respectively. So, previous studies were mainly focused on the effect of single lignocellulosic biomass on laccase activity.…”

“…In other words, the biosynthetic potential of Basidiomycetes was highly dependent on the species of fungi (Han et al, 2021a). An et al (2020b) reported that the capacity of secreting laccase of P. ostreatus strains was superior to F. velutipes strains due to the maximum laccase production on cottonseed hull, corncob, and poplar wood.…”

“…Agricultural and forestry waste is an important kind of lignocellulosic biomass. Lignocellulosic biomass, versatile and most abundant natural renewable resources, have attracted more attention and considerable interest due to their abilities to convert into green second-generation energy fuels and valued industrial chemicals via the various bio-treatment process of lignocellulosic biomass (Haldar et al, 2016;Pinar et al, 2017;An et al, 2020b;Gaikwad and Meshram, 2020). Using various lignocellulosic wastes, such as corncob, cottonseed hull, bamboos, coffee shell, and tree leaves, to produce enzymes (especially laccase) had been widely investigated in recent years due to the low-cost and vast amounts of lignocellulosic wastes (Lizardi-Jimenez et al, 2019;Thamvithayakorn et al, 2019;Wang et al, 2019;Agrawal and Verma, 2020;Atilano-Camino et al, 2020;Pinheiro et al, 2020;Xu et al, 2020).…”

Evaluation of laccase production by two white-rot fungi using solid-state fermentation with different agricultural and forestry residues

“…Because of the difference of laccase activity on different lignocellulosic biomass by fungi, it is important to study the laccase activity among different agricultural and forestry residues. Previous studies on Pleurotus ostreatus and Ganoderma lingzhi focused on laccase production induced by metals, temperature, and grown on two or three substrates (Elissetche et al 2007;Postemsky et al 2017;An et al 2020b;Han et al 2020b). Hence, this paper presents evaluation of laccase production by P. ostreatus CY 568 and G. lucidum Han 500 in various agricultural and forestry residues.…”

“…Moreover, type of fungal species or strains is an important factor affecting laccase production. Laccase activity of different strains from the genera, Pleurotus and Flammulina on substrate of wood, tree leaves, corncob, and wheat straw have been investigated (An et al 2020b;Han et al 2017Han et al , 2020b. Laccase production of Ganoderma lucidum has been discussed in the effect of rice and sunflower agro-residues, phenolic, and metallic inducers (Kuhar and Papinutti 2014;You et al 2014;Postemsky et al 2017;Palazzolo et al 2019).…”