“…The reference strain collection included bacteriocin-producer strains, which are Pa strains known to produce the following pyocins: S1 and R4 (PML28), AP41 and F3 (PAF41), S2, R and F (NIH18), and S2, R2 and F2 (PAO1) (Ito et al , 1970; Kuroda & Kageyama, 1981; Nakayama et al , 2000; Sano et al , 1990; Seo & Galloway, 1990). The reference collection also included indicator strains sensitive to various combinations of pyocins: PML1516d (S1 S S2 S AP41 S ), NIH3 (S1 S S2 S AP41 S ), NIH3S1 R (S1 R S2 S AP41 S ), NIH3S2 R (S1 S S2 R AP41 S ), NIH3AP41 R (S1 S S2 S AP41 R ), 3295 (AP41 S ), 3012 (AP41 S F3 S ), 7NSK2 (S1 S S3 S ), 7NSK2- fpvA (S1 S S3 R ), PML14 (S1 S R1 S R2 S R3 S R4 S R5 S ), 13s (S1 S R1 S R2 S R3 S R4 S R5 S ) and NIH5 (ATCC 25317) (F1 S F2 S F3 S ) (de Chial et al , 2003; Kageyama et al , 1979; Kuroda & Kageyama, 1981; Sano & Kageyama, 1981; Sano et al , 1993a; Williams et al , 2008). Four cloned S pyocins (S1, S2, S3 and AP41) were employed in this bacteriocin identification scheme (Duport et al , 1995; Sano & Kageyama, 1993; Sano et al , 1993b).…”