Role of bacteriocins in mediating interactions of bacterial isolates taken from cystic fibrosis patients

Bakkal, Suphan; Robinson, Sandra; Ordoñez, Claudia L.; Waltz, David A.; Riley, Margaret A.

doi:10.1099/mic.0.036848-0

Cited by 72 publications

(71 citation statements)

References 52 publications

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“…Possibly, fermenting Bcc bacteria exhibit unique mechanisms of resistance to pyocyanins. Bcc bacteria may produce their own bacteriocins under anaerobic conditions (77,78), whose identities remain unknown, enabling these bacteria to coexist with P. aeruginosa. Interestingly, Sass et al (51) recently discovered a gene cluster (designated the low-oxygen-activated [lxa] locus) that was significantly upregulated in Bcc bacteria during growth under low-oxygen conditions.…”

Section: Discussionmentioning

confidence: 99%

Localization of Burkholderia cepacia Complex Bacteria in Cystic Fibrosis Lungs and Interactions with Pseudomonas aeruginosa in Hypoxic Mucus

et al. 2014

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eThe localization of Burkholderia cepacia complex (Bcc) bacteria in cystic fibrosis (CF) lungs, alone or during coinfection with Pseudomonas aeruginosa, is poorly understood. We performed immunohistochemistry for Bcc and P. aeruginosa bacteria on 21 coinfected or singly infected CF lungs obtained at transplantation or autopsy. Parallel in vitro experiments examined the growth of two Bcc species, Burkholderia cenocepacia and Burkholderia multivorans, in environments similar to those occupied by P. aeruginosa in the CF lung. Bcc bacteria were predominantly identified in the CF lung as single cells or small clusters within phagocytes and mucus but not as "biofilm-like structures." In contrast, P. aeruginosa was identified in biofilm-like masses, but densities appeared to be reduced during coinfection with Bcc bacteria. Based on chemical analyses of CF and non-CF respiratory secretions, a test medium was defined to study Bcc growth and interactions with P. aeruginosa in an environment mimicking the CF lung. When test medium was supplemented with alternative electron acceptors under anaerobic conditions, B. cenocepacia and B. multivorans used fermentation rather than anaerobic respiration to gain energy, consistent with the identification of fermentation products by high-performance liquid chromatography (HPLC). Both Bcc species also expressed mucinases that produced carbon sources from mucins for growth. In the presence of P. aeruginosa in vitro, both Bcc species grew anaerobically but not aerobically. We propose that Bcc bacteria (i) invade a P. aeruginosa-infected CF lung when the airway lumen is anaerobic, (ii) inhibit P. aeruginosa biofilm-like growth, and (iii) expand the host bacterial niche from mucus to also include macrophages.

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Section: Discussionmentioning

confidence: 99%

Localization of Burkholderia cepacia Complex Bacteria in Cystic Fibrosis Lungs and Interactions with Pseudomonas aeruginosa in Hypoxic Mucus

et al. 2014

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“…Similarly, contact-dependent killing has been found to be responsive to the behaviour of competitors in a tit-for-tat fashion [77]. Although most of this work has been elucidated in an in vitro setting, multiple competitive phenotypes can be isolated from a host population [78] or a host individual [79]. Moreover, receptors for these molecules demonstrate high levels of diversifying selection [80].…”

Section: Key Examples Linking Within-host Competitive Interactions Anmentioning

confidence: 99%

Within-host competitive interactions as a mechanism for the maintenance of parasite diversity

Bashey

2015

Phil. Trans. R. Soc. B

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Variation among parasite strains can affect the progression of disease or the effectiveness of treatment. What maintains parasite diversity? Here I argue that competition among parasites within the host is a major cause of variation among parasites. The competitive environment within the host can vary depending on the parasite genotypes present. For example, parasite strategies that target specific competitors, such as bacteriocins, are dependent on the presence and susceptibility of those competitors for success. Accordingly, which parasite traits are favoured by within-host selection can vary from host to host. Given the fluctuating fitness landscape across hosts, genotype by genotype (G×G) interactions among parasites should be prevalent. Moreover, selection should vary in a frequency-dependent manner, as attacking genotypes select for resistance and genotypes producing public goods select for cheaters. I review competitive coexistence theory with regard to parasites and highlight a few key examples where within-host competition promotes diversity. Finally, I discuss how within-host competition affects host health and our ability to successfully treat infectious diseases.

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“…However, P. aeruginosa is unable to utilize ornibactin for iron acquisition (Weaver & Kolter, 2004). Bakkal et al (2010) have reported that bacteriocins produced by Pseudomonas and Burkholderia strains have intra-and interspecies bacteriocin-like inhibition ability within the CF lung (Bakkal et al, 2010). Some of these bacteriocins, such as the Pseudomonas pyocin, share the same receptors as siderophores (Denayer et al, 2007;Elfarash et al, 2014) and therefore interfere with iron acquisition of their target host.…”

Section: Pathogen Interactions and Competition For Iron In Cfmentioning

confidence: 99%

Iron acquisition in the cystic fibrosis lung and potential for novel therapeutic strategies

Tyrrell¹,

Callaghan²

2016

Microbiology

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Iron acquisition is vital to microbial survival and is implicated in the virulence of many of the pathogens that reside in the cystic fibrosis (CF) lung. The multifaceted nature of iron acquisition by both bacterial and fungal pathogens encompasses a range of conserved and speciesspecific mechanisms, including secretion of iron-binding siderophores, utilization of siderophores from other species, release of iron from host iron-binding proteins and haemoproteins, and ferrous iron uptake. Pathogens adapt and deploy specific systems depending on iron availability, bioavailability of the iron pool, stage of infection and presence of competing pathogens. Understanding the dynamics of pathogen iron acquisition has the potential to unveil new avenues for therapeutic intervention to treat both acute and chronic CF infections. Here, we examine the range of strategies utilized by the primary CF pathogens to acquire iron and discuss the different approaches to targeting iron acquisition systems as an antimicrobial strategy.

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Role of bacteriocins in mediating interactions of bacterial isolates taken from cystic fibrosis patients

Cited by 72 publications

References 52 publications

Localization of Burkholderia cepacia Complex Bacteria in Cystic Fibrosis Lungs and Interactions with Pseudomonas aeruginosa in Hypoxic Mucus

Localization of Burkholderia cepacia Complex Bacteria in Cystic Fibrosis Lungs and Interactions with Pseudomonas aeruginosa in Hypoxic Mucus

Within-host competitive interactions as a mechanism for the maintenance of parasite diversity

Iron acquisition in the cystic fibrosis lung and potential for novel therapeutic strategies

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