Comparative Study on DNA Quantification of Three Indian Major Carps of Middle Ganga Region

Abstract

The present study was conducted on DNA extraction and determination of DNA quantity of both male and female of Indian major carps Catla catla, Cirrhinus mrigala and Labeo rohita collected from three different sites of western Uttar Pradesh, India. DNA was isolated and gel electrophoresis was carried out and extracted DNA was analyzed using Nanophotometer to determine the quantity of DNA and its purity level. A total of 90 samples were analyzed. The DNA content of Catla catla female was 62-80 ng/μl and of male was 71-84 ng/μl; of Cirrhinus mrigala female was 62-74 ng/μl and of male was 64-78 ng/μl and of Labeo rohita female was 59-68 ng/μl and of male was 66-74 ng/μl. For evaluation of interspecific variations, the DNA content variation among sex, type (species), site were analyzed using generalized linear model by the analysis of the content variation which was done using ANOVA (Univariate Analysis of Variance UNIANOVA using SPSS 20.0, Followed by Posthoc Duncan's Multiple Range Test) and showed significance at 1% level in all parameters (site, type, sex).