Comparative study of various growth factors and cytokines on type I collagen and hyaluronan production in human dermal fibroblasts

Kim, Mi‐Sun; Song, Hye Jin; Lee, Sang Hwa; Lee, Cheon Koo

doi:10.1111/jocd.12073

Cited by 61 publications

(47 citation statements)

References 31 publications

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“…H,I). These findings are in agreement with reports showing stimulation of collagen production in vitro by rhIL-15 (Kim et al ., 2014) and PPAR agonists (Ham et al ., 2010) and demonstrate that physiologic elevations of circulating IL-15 can mimic some of beneficial effects of exercise training on aging skin and skeletal muscle metabolism. Furthermore, as inflammation is a hallmark of aging and metabolic dysfunction (Green et al ., 2011), we measured plasma cytokines from each treatment group and found that rmIL-15 and exercise treatment reduced circulating levels of the inflammatory cytokines IL-6 and MCP-1 (Fig6J,K).…”

Section: Resultsmentioning

confidence: 99%

Exercise‐stimulated interleukin‐15 is controlled byAMPKand regulates skin metabolism and aging

et al. 2015

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Aging is commonly associated with a structural deterioration of skin that compromises its barrier function, healing, and susceptibility to disease. Several lines of evidence show that these changes are driven largely by impaired tissue mitochondrial metabolism. While exercise is associated with numerous health benefits, there is no evidence that it affects skin tissue or that endocrine muscle-to-skin signaling occurs. We demonstrate that endurance exercise attenuates age-associated changes to skin in humans and mice and identify exercise-induced IL-15 as a novel regulator of mitochondrial function in aging skin. We show that exercise controls IL-15 expression in part through skeletal muscle AMP-activated protein kinase (AMPK), a central regulator of metabolism, and that the elimination of muscle AMPK causes a deterioration of skin structure. Finally, we establish that daily IL-15 therapy mimics some of the anti-aging effects of exercise on muscle and skin in mice. Thus, we elucidate a mechanism by which exercise confers health benefits to skin and suggest that low-dose IL-15 therapy may prove to be a beneficial strategy to attenuate skin aging.

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Section: Resultsmentioning

confidence: 99%

Exercise‐stimulated interleukin‐15 is controlled byAMPKand regulates skin metabolism and aging

et al. 2015

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“…PDGF induces dermal fibroblasts to produce collagen during wound healing[110]. PDGF incorporated into skin scaffolds improve wound healing due to increased collagen deposition[111].…”

Section: Components Of a Skin Substitutementioning

confidence: 99%

Methodologies in creating skin substitutes

Nicholas

Jeschke

Amini-Nik

2016

Cell. Mol. Life Sci.

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The creation of skin substitutes has significantly decreased morbidity and mortality of skin wounds. Although there are still a number of disadvantages of currently available skin substitutes, there has been a significant decline in research advances over the past several years in improving these skin substitutes. Clinically most skin substitutes used are acellular and do not use growth factors to assist wound healing, key areas of potential in this field of research. This article discusses the five necessary attributes of an ideal skin substitute. It comprehensively discusses the three major basic components of currently available skin substitutes: scaffold materials, growth factors, and cells, comparing and contrasting what has been used so far. It then examines a variety of techniques in how to incorporate these basic components together to act as a guide for further research in the field to create cellular skin substitutes with clinically better results.

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“…Besides the participation of MCP-1 in early inflammation, additional functions during the process of wound healing are discussed, there under the influence of MCP-1 on extracellular matrix degradation by matrix metalloproteinases, which is also an important step during the process of angiogenesis within the healing sequence [24]. Not only matrix degradation, but also the synthesis of collagens and hyaluronan by fibroblasts seems to be triggered by MCP-1 during skin remodelling [24,25]. We also focused on MCP-1 in this study because there is a strong link of MCP-1 over-expression and fibrosis development in different organs [26], an aspect with high relevance for soft tissue repair [11].…”

Section: Introductionmentioning

confidence: 99%

Response of endothelial cells and pericytes to hypoxia and erythropoietin in a co-culture assay dedicated to soft tissue repair

et al. 2015

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The increasing mean life expectancy of the citizens of the western world countries leads to an increase of the age-related diseases, among them soft tissue defects exhibiting inadequate healing. In order to develop new therapeutic strategies to support disturbed soft tissue repair, there is a strong need of sophisticated in vitro assays. A new assay combining scratch wounding with co-cultures of primary human microvascular endothelial cells (HDMEC) and pericytes (HPC) focuses on basic characteristics of cell interaction against the background of soft tissue repair. The cell parameters proliferation, migration and differentiation, and the release of monocyte chemoattractant protein-1 (MCP-1) were analysed in response to hypoxia (pO2 < 5 mmHg) and to erythropoietin (EPO; 50 IU/ml), a glycoprotein hormone having shown promising effects in soft tissue repair. As basic characteristics of the assay, direct cell contact in co-culture led to a weakened proliferation of both cell types, an increase of the percentage of myofibroblast-like pericytes and to a higher release of MCP-1. Hypoxia caused a proliferation decrease of HPC in co-culture, which was slightly attenuated by EPO. Hypoxia also reduced the MCP-1 release of co-cultured cells, when EPO had been added. In addition, EPO had a rather positive effect on HPC migration under hypoxia. These in vitro results allow new insights into the interaction of pericytes with endothelial cells in the context of soft tissue repair.

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Comparative study of various growth factors and cytokines on type I collagen and hyaluronan production in human dermal fibroblasts

Cited by 61 publications

References 31 publications

Exercise‐stimulated interleukin‐15 is controlled byAMPKand regulates skin metabolism and aging

Exercise‐stimulated interleukin‐15 is controlled byAMPKand regulates skin metabolism and aging

Methodologies in creating skin substitutes

Response of endothelial cells and pericytes to hypoxia and erythropoietin in a co-culture assay dedicated to soft tissue repair

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