Comparative study of tumor hypoxia by diffuse optical spectroscopy and immunohistochemistry in two tumor models

Maslennikova, Anna; Orlova, Anna; Golubiatnikov, G. Yu.; Kamensky, Vladislav A.; Shakhova, Natalia M.; Babaev, A. A.; Snopova, Ludmila B.; Иванова, И. П.; Плеханов, В. И.; Prianikova, Tatyana I.; Turchin, Ilya V.

doi:10.1002/jbio.201000060

Cited by 11 publications

(6 citation statements)

References 33 publications

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“…In our work, it was demonstrated that single irradiation of Pliss' lymph sarcoma at a dose of 10 Gy leads in 24 h after exposure to a statistically significant inhibition of tumor growth comparing to non-irradiated control (figure 2), and to a significant change of its oxygenation. Blood oxygen saturation that is consistent to tumor oxygenation [40] increased as compared to the initial level as well as to the nonirradiated control (figure 3). The results were in a good accordance with the data obtained in the study of radiation induced changes of tumor oxygen state using the EPR-oximetry [9].…”

Section: Discussionmentioning

confidence: 69%

“…It is shown that the hypoxia-induced factor HIF-1 plays the main role in the mechanism of such protective effect [45]. It is possible that the reoxygenation originated from improved tumor perfusion and increase in oxyhemoglobin concentration after irradiation, since a large number of blood vessels is a characteristic feature of Pliss' lymphosarcoma [40].…”

Section: Discussionmentioning

confidence: 99%

“…Determination of tissue components distribution from DOS measurements was performed using a custom-developed Mathcad software, as described in [40]. Spatial distributions of absorption and reduced scattering coefficients were reconstructed from the raw data acquired by the DOS setup: amplitude and phase of the photon density waves at three wavelengths.…”

Section: Diffuse Optical Spectroscopy Systemmentioning

confidence: 99%

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Diffuse optical spectroscopy assessment of rodent tumor model oxygen state after single-dose irradiation

Orlova

Maslennikova

Golubiatnikov

et al. 2019

Biomed. Phys. Eng. Express

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Modern radiation therapy of malignant tumors requires careful selection of conditions that can improve the effectiveness of the treatment. The study of the dynamics and mechanisms of tumor reoxygenation after radiation therapy makes it possible to select the regimens for optimizing the ongoing treatment. Diffuse optical spectroscopy (DOS) is among the methods used for non-invasive assessment of tissue oxygenation. In this work DOS was used for in vivo registration of changes in oxygenation level of an experimental rat tumor after single-dose irradiation at a dose of 10 Gy and investigation of their possible mechanisms. It was demonstrated that in 24 h after treatment, tumor oxygenation increases, which is mainly due to an increase in the oxygen supply to the tissues. DOS is demonstrated to be efficient for study of changes in blood flow parameters when monitoring tumor response to therapy.

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Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Diffuse Optical Spectroscopy Systemmentioning

confidence: 99%

See 1 more Smart Citation

Diffuse optical spectroscopy assessment of rodent tumor model oxygen state after single-dose irradiation

Orlova

Maslennikova

Golubiatnikov

et al. 2019

Biomed. Phys. Eng. Express

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“…In addition, abnormalities of tumor vascular bed may become the reason of venous blood outflow disorders [29]. High level of HHb in the aggressively growing tumor has been demonstrated earlier for rat model [30].…”

Section: Discussionmentioning

confidence: 87%

Diffuse optical spectroscopy monitoring of oxygen state and hemoglobin concentration during SKBR-3 tumor model growth

et al. 2016

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Tumor oxygenation and hemoglobin content are the key indicators of the tumor status which can be efficiently employed for prognosis of tumor development and choice of treatment strategy. We report on monitoring of these parameters in SKBR-3 (human breast adenocarcinoma) tumors established as subcutaneous tumor xenografts in athymic nude mice by diffuse optical spectroscopy (DOS). A simple continuous wave fiber probe DOS system is employed. Optical properties extraction approach is based on diffusion approximation. Statistically significant difference between measured values of normal tissue and tumor are demonstrated. Hemoglobin content in tumor increases from 7.0 ± 4.2 μM to 30.1 ± 16.1 μM with tumor growth from 150 ± 80 mm 3 to 1300 ± 650 mm 3 which is determined by gradual increase of deoxyhemoglobin content while measured oxyhemoglobin content does not demonstrate any statistically significant variations. Oxygenation in tumor falls quickly from 52.8 ± 24.7% to 20.2 ± 4.8% preceding acceleration of tumor growth. Statistical analysis indicated dependence of oxy-, deoxy-and total hemoglobin on tumor volume ( p < 0.01). DOS measurements of oxygen saturation are in agreement with independent measurements of oxygen partial pressure by polarography (Pearson's correlation coefficient equals 0.8).

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“…Diffuse optical imaging has been widely applied to biological tissue, for applications including the identification of skin diseases and monitoring the concentration of various chromophores . The technique of spatially resolved diffuse imaging has typically involved a single light source incident on the tissue surface, with either an array of point photodetectors or a camera‐based system to capture the resulting spatial distribution of light.…”

Section: Introductionmentioning

confidence: 99%

Spatially resolved diffuse imaging for high‐speed depth estimation of jet injection

Brennan

Ruddy

Nielsen

et al. 2019

Journal of Biophotonics

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We investigate the use of spatially resolved diffuse imaging to track a fluid jet delivered at high speed into skin tissue. A jet injector with a short needle to deliver drugs beneath the dermis, is modified to incorporate a laser beam into the jet, which is ejected into ex vivo porcine tissue. The diffuse light emitted from the side and top of the tissue sample is recorded using high‐speed videography. Similar experiments, using a depth‐controlled fiber optic source, generate a reference dataset. The side light distribution is related to source depth for the controlled‐source experiments and used to track the effective source depth of the injections. Postinjection X‐ray images show agreement between the jet penetration and ultimate light source depth. The surface light intensity profile is parameterized with a single parameter and an exponential function is used to relate this parameter to source depth for the controlled‐source data. This empirical model is then used to estimate the effective source depth from the surface profile of the injection experiments. The depth estimates for injections into fat remain close to the side depth estimates, with a root‐mean‐square error of 1.1 mm, up to a source depth of 8 mm.

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Comparative study of tumor hypoxia by diffuse optical spectroscopy and immunohistochemistry in two tumor models

Cited by 11 publications

References 33 publications

Diffuse optical spectroscopy assessment of rodent tumor model oxygen state after single-dose irradiation

Diffuse optical spectroscopy assessment of rodent tumor model oxygen state after single-dose irradiation

Diffuse optical spectroscopy monitoring of oxygen state and hemoglobin concentration during SKBR-3 tumor model growth

Spatially resolved diffuse imaging for high‐speed depth estimation of jet injection

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